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Cited by 17Algal biology and biofuel productionLipid metabolism and biosynthesisMicrobial Metabolic Engineering and Bioproduction

Auxin supplementation under nitrogen limitation enhanced oleic acid and MUFA content in Eustigmatos calaminaris biomass with potential for biodiesel production

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Krzemińska, Izabela, Szymańska, Marlena, Ciempiel, Wioleta, Piasecka, Agata·2023·Scientific Reports
DOI 10.1038/s41598-023-27778-yPMC PMC9834312
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Abstract

Due to their lipid accumulation potential, microalgae are widely studied in terms of their use in the production of biodiesel. The present study was focused on determination of changes in the biomass production, biochemical composition, accumulation and distribution of fatty acids in neutral lipids, glycolipids, phospholipids and biodiesel properties of soil microalga Eustigmatos calaminaris in response to various levels of nitrogen stress and indole-3-acetic acid supplementation. The highest growth rate, the highest lipid content and daily lipid productivity were noted at the nitrogen limitation up to 25% with IAA supplementation. The increase in NL was associated with nutrient stress. An increase in the level of GL and PL were recorded upon the reduction of the nitrogen content (25% N) and the addition of IAA. The gas chromatography/mass spectrometry analysis demonstrated that C16:0, C16:1, and C18:1 were the main fatty acids in E. calaminaris lipids. As shown by the lipidomic analysis, the IAA supplementation in the nitrogen limitation variants enhanced the content of TAGs in C18:1 and monounsaturated fatty acids. The current findings indicated a potential strategy to improve the fatty acid profile in neutral lipids and high potential of E. calaminaris for biodiesel applications.

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Cited by

Sorted by citations

Authors
Krzemińska, I., Szymańska, M., Ciempiel, W., & Piasecka, A.
Affiliations
Institute of Agrophysics, Polish Academy of Sciences (PL)
Corresponding author
Krzemińska, Izabela
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2023-01-11
Volume
13
Issue
1
Pages
594-594
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-023-27778-y
PMC
PMC9834312
OpenAlex
W4315641632
Cited by
17
References
1
Citations by year
2026: 5 · 2025: 5 · 2024: 6 · 2023: 1
FWCI
0.81
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Narodowym Centrum Nauki
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
indole-3-acetic acid, nitrogen limitation, oleic acid, monounsaturated fatty acids, microalgal lipid accumulation, biodiesel feedstock, triglycerides, fatty acid composition, neutral lipids, glycolipids, phospholipids, soil microalgae, lipid productivity
MeSH terms
Fatty Acids, Indoleacetic Acids, Nitrogen, Biomass, Oleic Acid, Dietary Supplements, Biofuels, Microalgae

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