An outlook on microalgal biofuels

Wijffels, René H.; Barbosa, Maria J.

doi:10.1126/science.1189003

Cited by 1,886Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionLipid metabolism and biosynthesis

An outlook on microalgal biofuels

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Wijffels, René H., Barbosa, Maria J.·2010·Science

DOI 10.1126/science.1189003

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Abstract

Microalgae are considered one of the most promising feedstocks for biofuels. The productivity of these photosynthetic microorganisms in converting carbon dioxide into carbon-rich lipids, only a step or two away from biodiesel, greatly exceeds that of agricultural oleaginous crops, without competing for arable land. Worldwide, research and demonstration programs are being carried out to develop the technology needed to expand algal lipid production from a craft to a major industrial process. Although microalgae are not yet produced at large scale for bulk applications, recent advances-particularly in the methods of systems biology, genetic engineering, and biorefining-present opportunities to develop this process in a sustainable and economical way within the next 10 to 15 years.

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Chisti, Y. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.02.001 · Cited by 9,524 · see citing works
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745 · see citing works
The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions
Merchant, S., Prochnik, S., Vallon, O., Harris, E. H., Karpowicz, S. J., Witman, G., Terry, A., Salamov, A., Fritz-Laylin, L., Maréchal-Drouard, L., et al. (2007) · Science · doi:10.1126/science.1143609 · Cited by 2,765 · see citing works
Valuable Products from Biotechnology of Microalgae
Pulz, O., & Gross, W. (2004) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-004-1647-x · Cited by 2,132 · see citing works
The Genome of the Diatom Thalassiosira Pseudonana : Ecology, Evolution, and Metabolism
Armbrust, E. V., Berges, J. A., Bowler, C., Green, B. R., Martínez, D., Putnam, N. H., Zhou, S., Allen, A. E., Apt, K. E., Bechner, M., et al. (2004) · Science · doi:10.1126/science.1101156 · Cited by 2,029 · see citing works
Look Back at the U.S. Department of Energy's Aquatic Species Program: Biodiesel from Algae; Close-Out Report
Sheehan, J., Dunahay, T., Benemann, J., & Roessler, P. (1998) · doi:10.2172/15003040 · Cited by 1,753 · see citing works
The Phaeodactylum genome reveals the evolutionary history of diatom genomes
Bowler, C., Allen, A. E., Badger, J., Grimwood, J., Jabbari, K., Kuo, A., Maheswari, U., Martens, C., Maumus, F., Otillar, R., et al. (2008) · Nature · doi:10.1038/nature07410 · Cited by 1,656 · see citing works
Life-Cycle Assessment of Biodiesel Production from Microalgae
Lardon, L., Hélias, A., Sialve, B., Steyer, J.-P., & Bernard, O. (2009) · Environmental Science & Technology · doi:10.1021/es900705j · Cited by 1,528 · see citing works
Solar energy conversion efficiencies in photosynthesis: Minimizing the chlorophyll antennae to maximize efficiency
Melis, A. (2009) · Plant Science · doi:10.1016/j.plantsci.2009.06.005 · Cited by 796 · see citing works
Botryococcus braunii : A Renewable Source of Hydrocarbons and Other Chemicals
Banerjee, A., Sharma, R., Yusuf Chisti, B., & Banerjee, U. C. (2002) · Critical Reviews in Biotechnology · doi:10.1080/07388550290789513 · Cited by 714 · see citing works
Microalgae for the production of bulk chemicals and biofuels
Wijffels, R. H., Barbosa, M. J., & Eppink, M. H. M. (2010) · Biofuels, Bioproducts and Biorefining · doi:10.1002/bbb.215 · Cited by 522 · see citing works
Self-citation
Ethanol Synthesis by Genetic Engineering in Cyanobacteria
Deng, M.-D., & Coleman, J. R. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.2.523-528.1999 · Cited by 457 · see citing works
Photobiology of microalgae mass cultures: understanding the tools for the next green revolution
Tredici, M. R. (2010) · Biofuels · doi:10.4155/bfs.09.10 · Cited by 449 · see citing works
Biofuels and implications for agricultural water use: blue impacts of green energy
de Fraiture, C., Giordano, M., & Liao, Y. (2008) · Water Policy · doi:10.2166/wp.2008.054 · Cited by 432 · see citing works
Biological Transformation of Solar Energy
Oswald, W. J., & Golueke, C. G. (1960) · Advances in applied microbiology · doi:10.1016/s0065-2164(08)70127-8 · Cited by 388 · see citing works
Hydrogen production by microalgae
Benemann, J. R. (2000) · Journal of Applied Phycology · doi:10.1023/a:1008175112704 · Cited by 263 · see citing works
Transforming Triglycerides and Fatty Acids into Biofuels
Lestari, S., Mäki‐Arvela, P., Beltramini, J. N., Lu, G. Q., & Murzin, D. Y. (2009) · ChemSusChem · doi:10.1002/cssc.200900107 · Cited by 260 · see citing works
Productivity and photosynthetic efficiency ofSpirulina platensis as affected by light intensity, algal density and rate of mixing in a flat plate photobioreactor
Qiang, H., & RICHMOND, A. E. (1996) · Journal of Applied Phycology · doi:10.1007/bf02186317 · Cited by 258 · see citing works
Photosynthetic efficiency of Chlorella sorokiniana in a turbulently mixed short light‐path photobioreactor
Kliphuis, A. M. J., de Winter, L., Vejrazka, C., Martens, D. E., Janssen, M., & Wijffels, R. H. (2010) · Biotechnology Progress · doi:10.1002/btpr.379 · Cited by 145 · see citing works
Self-citation
Maximum Photosynthetic Yield of Green Microalgae in Photobioreactors
Zijffers, J.-W. F., Schippers, K. J., Zheng, K., Janssen, M., Tramper, J., & Wijffels, R. H. (2010) · Marine Biotechnology · doi:10.1007/s10126-010-9258-2 · Cited by 137 · see citing works
Self-citation
ALGAL TRANSGENICS IN THE GENOMIC ERA1
Walker, T. L., Collet, C., & Purton, S. (2005) · Journal of Phycology · doi:10.1111/j.1529-8817.2005.00133.x · Cited by 137 · see citing works
The biological CO2 fixation and utilization project by RITE(1) — Highly-effective photobioreactor system —
Usui, N., & Ikenouchi, M. (1997) · Energy Conversion and Management · doi:10.1016/s0196-8904(96)00315-9 · Cited by 107 · see citing works
The European Nitrogen Case
van Egmond, K., Bresser, T. H. M., & Bouwman, L. (2002) · AMBIO · doi:10.1639/0044-7447(2002)031[0072:tenc]2.0.co;2 · Cited by 91 · see citing works
Do biofuels from microalgae beat biofuels from terrestrial plants?
Reijnders, L. (2008) · Trends in biotechnology · doi:10.1016/j.tibtech.2008.04.001 · Cited by 87 · see citing works
The European Nitrogen Case
van Egmond, K., Bresser, T. H. M., & Bouwman, L. (2002) · AMBIO · doi:10.1579/0044-7447-31.2.72 · Cited by 87 · see citing works
Towards increased microalgal productivity in photobioreactors
Bosma, R., Vermuë, M. H., TRAMPER, J., et al. (2010) · Socio-Environmental Systems Modeling · Cited by 10 · see citing works

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Aggregation-Induced Emission: Together We Shine, United We Soar!
Mei, J., Leung, N. L. C., Kwok, R. T. K., Lam, J. W. Y., & Tang, B. Z. (2015) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00263 · Cited by 8,270 · see citing works
Comparing Photosynthetic and Photovoltaic Efficiencies and Recognizing the Potential for Improvement
Blankenship, R. E., Tiede, D. M., Barber, J., Brudvig, G. W., Fleming, G., Ghirardi, M., Gunner, M. R., Junge, W., Kramer, D. M., Melis, A., et al. (2011) · Science · doi:10.1126/science.1200165 · Cited by 1,638 · see citing works
High-value products from microalgae—their development and commercialisation
Borowitzka, M. A. (2013) · Journal of Applied Phycology · doi:10.1007/s10811-013-9983-9 · Cited by 1,585 · see citing works
Carbon capture, storage and utilisation technologies: A critical analysis and comparison of their life cycle environmental impacts
Cuéllar-Franca, R. M., & Azapagic, A. (2015) · Journal of CO2 Utilization · doi:10.1016/j.jcou.2014.12.001 · Cited by 1,571 · see citing works
Algal Proteins: Extraction, Application, and Challenges Concerning Production
Bleakley, S., & Hayes, M. (2017) · Foods · doi:10.3390/foods6050033 · Cited by 1,092 · see citing works
Extraction of oil from microalgae for biodiesel production: A review
Halim, R., Danquah, M. K., & Webley, P. A. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.01.001 · Cited by 1,043 · see citing works
Micro-algae cultivation for biofuels: Cost, energy balance, environmental impacts and future prospects
Slade, R., & Bauen, A. (2013) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2012.12.019 · Cited by 978 · see citing works
Flocculation as a low-cost method for harvesting microalgae for bulk biomass production
Vandamme, D., Foubert, I., & Muylaert, K. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2012.12.005 · Cited by 928 · see citing works
Microalgae: A potential alternative to health supplementation for humans
Koyande, A. K., Chew, K. W., Rambabu, K., Tao, Y., Chu, D.-T., & Show, P.-L. (2019) · Food Science and Human Wellness · doi:10.1016/j.fshw.2019.03.001 · Cited by 908 · see citing works
Microalgae Metabolites: A Rich Source for Food and Medicine
Sathasivam, R., Radhakrishnan, R., Hashem, A., & Abd_Allah, E. F. (2019) · Saudi Journal of Biological Sciences · doi:10.1016/j.sjbs.2017.11.003 · Cited by 872 · see citing works
Production cost of a real microalgae production plant and strategies to reduce it
Acién, F. G., Fernández, J. M., Magán, J. J., & Molina, E. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.02.005 · Cited by 735 · see citing works
Microalgae-based carbohydrates for biofuel production
Chen, C.-Y., Zhao, X.-Q., Yen, H.-W., Ho, S.-H., Cheng, C.-L., Lee, D.-J., Bai, F.-W., & Chang, J.-S. (2013) · Biochemical Engineering Journal · doi:10.1016/j.bej.2013.03.006 · Cited by 724 · see citing works
Constraints to Commercialization of Algal Fuels
Chisti, Y. (2013) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2013.07.020 · Cited by 719 · see citing works
Hydrothermal liquefaction (HTL) of microalgae for biofuel production: State of the art review and future prospects
López Barreiro, D., Prins, W., Ronsse, F., & Brilman, W. (2013) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2012.12.029 · Cited by 695 · see citing works
Microalgae as versatile cellular factories for valued products
Koller, M., Muhr, A., & Braunegg, G. (2014) · Algal Research · doi:10.1016/j.algal.2014.09.002 · Cited by 639 · see citing works
Temperature effect on microalgae: A crucial factor for outdoor production
Ras, M., Steyer, J.-P., & Bernard, O. (2013) · Reviews in Environmental Science and Bio/Technology · doi:10.1007/s11157-013-9310-6 · Cited by 532 · see citing works
Microalgal Biostimulants and Biofertilisers in Crop Productions
Ronga, D., Biazzi, E., Parati, K., Carminati, D., Carminati, E., & Tava, A. (2019) · Agronomy · doi:10.3390/agronomy9040192 · Cited by 536 · see citing works
The future supply of animal-derived protein for human consumption
Boland, M. J., Rae, A. N., Vereijken, J. M., Meuwissen, M. P. M., Fischer, A. R. H., van Boekel, M. A. J. S., Rutherfurd, S. M., Gruppen, H., Moughan, P. J., & Hendriks, W. H. (2013) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2012.07.002 · Cited by 524 · see citing works
Methods of downstream processing for the production of biodiesel from microalgae
Kim, J., Yoo, G., Lee, H., Lim, J., Kim, K., Kim, C. W., Park, M. S., & Yang, J.-W. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2013.04.006 · Cited by 519 · see citing works
Screening Microalgae Strains for Biodiesel Production: Lipid Productivity and Estimation of Fuel Quality Based on Fatty Acids Profiles as Selective Criteria
Nascimento, I. A., Marques, S. S. I., Cabanelas, I. T. D., Pereira, S. A., Druzian, J. I., de Souza, C. O., Vich, D. V., de Carvalho, G. C., & Nascimento, M. A. (2013) · BioEnergy Research · doi:10.1007/s12155-012-9222-2 · Cited by 517 · see citing works
Changes in Transcript Abundance in Chlamydomonas reinhardtii following Nitrogen Deprivation Predict Diversion of Metabolism
Miller, R., Wu, G., Deshpande, R. R., Vieler, A., Gärtner, K., Li, X., Moellering, E. R., Zäuner, S., Cornish, A. J., Liu, B., et al. (2010) · PLANT PHYSIOLOGY · doi:10.1104/pp.110.165159 · Cited by 511 · see citing works
Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779
Vieler, A., Wu, G., Tsai, C.-H., Bullard, B., Cornish, A. J., Harvey, C., Reca, I.-B., Thornburg, C., Achawanantakun, R., Buehl, C. J., et al. (2012) · PLoS Genetics · doi:10.1371/journal.pgen.1003064 · Cited by 458 · see citing works
Dual purpose microalgae–bacteria-based systems that treat wastewater and produce biodiesel and chemical products within a Biorefinery
Olguín, E. J. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.05.001 · Cited by 473 · see citing works
Impact of Microalgae-Bacteria Interactions on the Production of Algal Biomass and Associated Compounds
Fuentes, J., Garbayo, I., Cuaresma, M., Montero, Z., González-del-Valle, M., & Vílchez, C. (2016) · Marine Drugs · doi:10.3390/md14050100 · Cited by 462 · see citing works
Biofertilizer and biostimulant properties of the microalga Acutodesmus dimorphus
Garcia-Gonzalez, J., & Sommerfeld, M. (2016) · Journal of Applied Phycology · doi:10.1007/s10811-015-0625-2 · Cited by 403 · see citing works
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Liu, Y., Cruz-Morales, P., Zargar, A., Belcher, M. S., Pang, B., Englund, E., Dan, Q., Yin, K., & Keasling, J. D. (2021) · Cell · doi:10.1016/j.cell.2021.01.052 · Cited by 399 · see citing works
The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae
Rumin, J., Bonnefond, H., Saint-Jean, B., Rouxel, C., Sciandra, A., Bernard, O., Cadoret, J.-P., & Bougaran, G. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0220-4 · Cited by 386 · see citing works
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Ma, X.-N., Chen, T.-P., Yang, B., Liu, J., & Chen, F. (2016) · Marine Drugs · doi:10.3390/md14040061 · Cited by 394 · see citing works
Hydrothermal liquefaction for algal biorefinery: A critical review
Tian, C., Li, B., Liu, Z., Zhang, Y., & Lu, H. (2014) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2014.07.030 · Cited by 389 · see citing works
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Peng, B., Yuan, X., Zhao, C., & Lercher, J. A. (2012) · Journal of the American Chemical Society · doi:10.1021/ja302436q · Cited by 378 · see citing works
Combinatorial life cycle assessment to inform process design of industrial production of algal biodiesel
Brentner, L. B., Eckelman, M. J., & Zimmerman, J. B. (2011) · Environmental Science & Technology · doi:10.1021/es2006995 · Cited by 377 · see citing works
A chloroplast pathway for the de novo biosynthesis of triacylglycerol inChlamydomonas reinhardtii
Fan, J., Andre, C., & Xu, C. (2011) · FEBS Letters · doi:10.1016/j.febslet.2011.05.018 · Cited by 347 · see citing works
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Bernard, O., & Rémond, B. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.07.022 · Cited by 349 · see citing works
Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosaunder three nutrition stressors
Fan, J., Cui, Y., Wan, M., Wang, W., & Li, Y. (2014) · Biotechnology for Biofuels · doi:10.1186/1754-6834-7-17 · Cited by 341 · see citing works
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Vílchez, C., Forján, E., Cuaresma, M., Bédmar, F., Garbayo, I., & Vega, J. M. (2011) · Marine Drugs · doi:10.3390/md9030319 · Cited by 328 · see citing works
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Chauton, M. S., Reitan, K. I., Norsker, N. H., Tveterås, R., & Kleivdal, H. T. (2015) · Aquaculture · doi:10.1016/j.aquaculture.2014.10.038 · Cited by 323 · see citing works
Metabolism of acyl‐lipids in Chlamydomonas reinhardtii
Li‐Beisson, Y., Beisson, F., & Riekhof, W. (2015) · The Plant Journal · doi:10.1111/tpj.12787 · Cited by 310 · see citing works
Resource demand implications for us algae biofuels production scale-up
Pate, R., Klise, G., & Wu, B. (2011) · Applied Energy · doi:10.1016/j.apenergy.2011.04.023 · Cited by 315 · see citing works
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Wang, Q., Lu, Y., Xin, Y., Wei, L., Huang, S., & Xu, J. (2016) · The Plant Journal · doi:10.1111/tpj.13307 · Cited by 312 · see citing works
Dual potential of microalgae as a sustainable biofuel feedstock and animal feed
Lum, K. K., Kim, J., & Lei, X. G. (2013) · Journal of Animal Science and Biotechnology · doi:10.1186/2049-1891-4-53 · Cited by 276 · see citing works
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Breuer, G., Lamers, P. P., Martens, D. E., Draaisma, R. B., & Wijffels, R. H. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.05.105 · Cited by 270 · see citing works
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de Vree, J. H., Bosma, R., Janssen, M., Barbosa, M. J., & Wijffels, R. H. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0400-2 · Cited by 249 · see citing works
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Hoffman, J., Pate, R. C., Drennen, T., & Quinn, J. C. (2017) · Algal Research · doi:10.1016/j.algal.2017.01.005 · Cited by 241 · see citing works
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Adams, C., Godfrey, V., Wahlen, B., Seefeldt, L., & Bugbee, B. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2012.12.143 · Cited by 242 · see citing works
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Global evaluation of biofuel potential from microalgae
Moody, J. W., McGinty, C. M., & Quinn, J. C. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1321652111 · Cited by 235 · see citing works
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McGinn, P. J., Dickinson, K. E., Bhatti, S., Frigon, J.-C., Guiot, S. R., & O’Leary, S. J. B. (2011) · Photosynthesis Research · doi:10.1007/s11120-011-9638-0 · Cited by 230 · see citing works
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Garrido-Cardenas, J. A., Manzano-Agugliaro, F., Acien-Fernandez, F. G., & Molina-Grima, E. (2018) · Algal Research · doi:10.1016/j.algal.2018.08.005 · Cited by 216 · see citing works
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Choi, Y. Y., Patel, A. K., Hong, M. E., Chang, W. S., & Sim, S. J. (2019) · Bioresource Technology Reports · doi:10.1016/j.biteb.2019.100270 · Cited by 174 · see citing works
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Dong, H.-P., Williams, E., Wang, D.-Z., Xie, Z.-X., Hsia, R.-C., Jenck, A., Halden, R., Li, J., Chen, F., & Place, A. R. (2013) · Plant Physiology · doi:10.1104/pp.113.214320 · Cited by 173 · see citing works
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Fu, W., Gudmundsson, O., Feist, A. M., Herjolfsson, G., Brynjolfsson, S., & Palsson, B. Ø. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.07.004 · Cited by 170 · see citing works
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Correa, D. F., Beyer, H. L., Possingham, H. P., Thomas-Hall, S. R., & Schenk, P. M. (2017) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2017.02.068 · Cited by 161 · see citing works
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Vasudevan, V., Stratton, R. W., Pearlson, M. N., Jersey, G. R., Beyene, A. G., Weissman, J. C., Rubino, M., & Hileman, J. I. (2012) · Environmental Science & Technology · doi:10.1021/es2026399 · Cited by 157 · see citing works
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Olivieri, G., Salatino, P., & Marzocchella, A. (2014) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.4218 · Cited by 156 · see citing works
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Wang, J., Liu, J., & Liu, T. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0240-0 · Cited by 139 · see citing works
Effective cultivation of microalgae for biofuel production: A pilot-scale evaluation of a novel oleaginous microalga Graesiella sp. WBG-1
Wen, X., Du, K., Wang, Z., Peng, X., Luo, L., Tao, H., Xu, Y., Zhang, D., Geng, Y., & Li, Y. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0541-y · Cited by 142 · see citing works
Microalgal biomass production pathways: evaluation of life cycle environmental impacts
Zaimes, G. G., & Khanna, V. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-88 · Cited by 133 · see citing works
Comparison of Thraustochytrids Aurantiochytrium sp., Schizochytrium sp., Thraustochytrium sp., and Ulkenia sp. for Production of Biodiesel, Long-Chain Omega-3 Oils, and Exopolysaccharide
Lee Chang, K. J., Nichols, C. M., Blackburn, S. I., Dunstan, G. A., Koutoulis, A., & Nichols, P. D. (2014) · Marine Biotechnology · doi:10.1007/s10126-014-9560-5 · Cited by 133 · see citing works
Photobioreactor cultivation strategies for microalgae and cyanobacteria
Johnson, T. J., Katuwal, S., Anderson, G. A., Gu, L., Zhou, R., & Gibbons, W. R. (2018) · Biotechnology Progress · doi:10.1002/btpr.2628 · Cited by 128 · see citing works
Phytohormone addition coupled with nitrogen depletion almost tripled the lipid productivities in two algae
Yu, Z., Pei, H., Jiang, L., Hou, Q., Nie, C., & Zhang, L. (2018) · Bioresource Technology · doi:10.1016/j.biortech.2017.09.192 · Cited by 127 · see citing works
Photobioreactors for Mass Production of Microalgae
Zittelli, G. C., Biondi, N., Rodolfi, L., & Tredici, M. R. (2013) · Handbook of Microalgal Culture · doi:10.1002/9781118567166.ch13 · Cited by 155 · see citing works
Microalgal Cultivation for Value-Added Products: A Critical Enviro-Economical Assessment
Kothari, R., Pandey, A., Ahmad, S., Kumar, A., Pathak, V. V., & Tyagi, V. V. (2017) · 3 Biotech · doi:10.1007/s13205-017-0812-8 · Cited by 116 · see citing works
Attached cultivation for improving the biomass productivity of spirulina platensis
Zhang, L., Chen, L., Wang, J., Chen, Y., Gao, X., Zhang, Z., & Liu, T. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.01.025 · Cited by 115 · see citing works
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Yan, Q., & Pfleger, B. F. (2020) · Metabolic Engineering · doi:10.1016/j.ymben.2019.04.009 · Cited by 105 · see citing works
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Mao, X., Zhang, Y., Wang, X., & Liu, J. (2020) · Biotechnology for Biofuels · doi:10.1186/s13068-020-01714-y · Cited by 104 · see citing works
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Wang, H., Laughinghouse, H. D., Anderson, M. A., Chen, F., Willliams, E., Place, A. R., Zmora, O., Zohar, Y., Zheng, T., & Hill, R. T. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.06474-11 · Cited by 102 · see citing works
Assessment of Environmental Stresses for Enhanced Microalgal Biofuel Production â€“ An Overview
Cheng, D., & He, Q. (2014) · Frontiers in Energy Research · doi:10.3389/fenrg.2014.00026 · Cited by 106 · see citing works
Attachment between heterotrophic bacteria and microalgae influences symbiotic microscale interactions
Samo, T. J., Kimbrel, J. A., Nilson, D. J., Pett‐Ridge, J., Weber, P. K., & Mayali, X. (2018) · Environmental Microbiology · doi:10.1111/1462-2920.14357 · Cited by 87 · see citing works
A novel flat plate algal bioreactor with horizontal baffles: Structural optimization and cultivation performance
Wang, L.-L., Tao, Y., & Mao, X.-Z. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.04.100 · Cited by 86 · see citing works
Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo
Dahlin, L. R., Gerritsen, A. T., Henard, C. A., Van Wychen, S., Linger, J. G., Kunde, Y., Hovde, B. T., Starkenburg, S. R., Posewitz, M. C., & Guarnieri, M. T. (2019) · Communications Biology · doi:10.1038/s42003-019-0620-2 · Cited by 86 · see citing works
Efficient CO2 capture from the air for high microalgal biomass production by a bicarbonate Pool
Zhu, C., Zhai, X., Xi, Y., Wang, J., Kong, F., Zhao, Y., & Chi, Z. (2020) · Journal of CO2 Utilization · doi:10.1016/j.jcou.2019.12.023 · Cited by 84 · see citing works
DRUM: A new framework for metabolic modeling under non-balanced growth. Application to the carbon metabolism of unicellular microalgae
Baroukh, C., Muñoz-Tamayo, R., Steyer, J.-P., & Bernard, O. (2014) · PLoS ONE · doi:10.1371/journal.pone.0104499 · Cited by 80 · see citing works
Tubular Photobioreactors
Torzillo, G., & Chini Zittelli, G. (2015) · Algal Biorefineries · doi:10.1007/978-3-319-20200-6_5 · Cited by 79 · see citing works
Pretreatment optimization of the biomass of Microcystis aeruginosa for efficient bioethanol production
Khan, M. I., Lee, M. G., Shin, J. H., & Kim, J. D. (2017) · AMB Express · doi:10.1186/s13568-016-0320-y · Cited by 78 · see citing works
The synchronized cell cycle of Neochloris oleoabundans and its influence on biomass composition under constant light conditions
de Winter, L., Klok, A. J., Cuaresma Franco, M., Barbosa, M. J., & Wijffels, R. H. (2013) · Algal Research · doi:10.1016/j.algal.2013.09.001 · Cited by 76 · see citing works
Self-citation
Production of chemicals from microalgae lipids – status and perspectives
Hess, S. K., Lepetit, B., Kroth, P. G., & Mecking, S. (2018) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201700152 · Cited by 74 · see citing works
Meta-analysis and harmonization of life cycle assessment studies for algae biofuels
Tu, Q., Eckelman, M., & Zimmerman, J. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.7b01049 · Cited by 75 · see citing works
Modeling method for combining fluid dynamics and algal growth in a bubble column photobioreactor
Nauha, E. K., & Alopaeus, V. (2013) · Chemical Engineering Journal · doi:10.1016/j.cej.2013.06.065 · Cited by 71 · see citing works
Biotechnological production of value-added carotenoids from microalgae: Emerging technology and prospects
Wichuk, K., Brynjólfsson, S., & Fu, W. (2014) · Bioengineered · doi:10.4161/bioe.28720 · Cited by 70 · see citing works
Exploring a blue-light-sensing transcription factor to double the peak productivity of oil in Nannochloropsis oceanica
Zhang, P., Xin, Y., He, Y., Tang, X., Shen, C., Wang, Q., Lv, N., Li, Y., Hu, Q., & Xu, J. (2022) · Nature Communications · doi:10.1038/s41467-022-29337-x · Cited by 67 · see citing works
Production of carbohydrates, lipids and polyunsaturated fatty acids (PUFA) by the polar marine microalga Chlamydomonas malina RCC2488
Morales-Sánchez, D., Schulze, P. S. C., Kiron, V., & Wijffels, R. H. (2020) · Algal Research · doi:10.1016/j.algal.2020.102016 · Cited by 63 · see citing works
Self-citation
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Quinn, J. C., Catton, K. B., Johnson, S., & Bradley, T. H. (2013) · BioEnergy Research · doi:10.1007/s12155-012-9277-0 · Cited by 67 · see citing works
Autotrophic Microalgae Biomass Production: From Niche Markets to Commodities
Benemann, J. R., Woertz, I., & Lundquist, T. (2018) · Industrial Biotechnology · doi:10.1089/ind.2018.29118.jrb · Cited by 65 · see citing works
Direct production of aviation fuels from microalgae lipids in water
Fu, J., Yang, C., Wu, J., Zhuang, J., Hou, Z., & Lu, X. (2015) · Fuel · doi:10.1016/j.fuel.2014.09.025 · Cited by 63 · see citing works
Coherent anti-Stokes Raman scattering and spontaneous Raman spectroscopy and microscopy of microalgae with nitrogen depletion
He, X. N., Allen, J., Black, P. N., Baldacchini, T., Huang, X., Huang, H., Jiang, L., & Lu, Y. F. (2012) · Biomedical Optics Express · doi:10.1364/boe.3.002896 · Cited by 62 · see citing works
Innovative harvesting processes for microalgae biomass production: A perspective from patent literature
Deconinck, N., Muylaert, K., Ivens, W., & Vandamme, D. (2018) · Algal Research · doi:10.1016/j.algal.2018.01.016 · Cited by 60 · see citing works
Microalgae production as a biofuel feedstock: Risks and challenges
Zhu, L., & Ketola, T. (2012) · International Journal of Sustainable Development & World Ecology · doi:10.1080/13504509.2011.636083 · Cited by 59 · see citing works
Accelerated triacylglycerol production and altered fatty acid composition in oleaginous microalga Neochloris oleoabundans by overexpression of diacylglycerol acyltransferase 2
Klaitong, P., Fa-aroonsawat, S., & Chungjatupornchai, W. (2017) · Microbial Cell Factories · doi:10.1186/s12934-017-0677-x · Cited by 58 · see citing works
Advancements and Prospects in Algal Biofuel Production: A Comprehensive Review
Falfushynska, H. (2024) · Phycology · doi:10.3390/phycology4040030 · Cited by 55 · see citing works
Comprehensive computational model for combining fluid hydrodynamics, light transport and biomass growth in a Taylor vortex algal photobioreactor: Eulerian approach
Gao, X., Kong, B., & Dennis Vigil, R. (2017) · Algal Research · doi:10.1016/j.algal.2017.03.009 · Cited by 53 · see citing works
Tropical Marine Chlorella sp. PP1 as a Source of Photosynthetic Pigments for Dye-Sensitized Solar Cells
Nurachman, Z., H, H., Rahmaniyah, W. R., Kurnia, D., Hidayat, R., Prijamboedi, B., Suendo, V., Ratnaningsih, E., Panggabean, L. M. G., & Nurbaiti, S. (2015) · Algal Research · doi:10.1016/j.algal.2015.04.009 · Cited by 55 · see citing works
Proteomic and biophysical analyses reveal a metabolic shift in nitrogen deprived Nannochloropsis oculata
Tran, N.-A. T., Padula, M. P., Evenhuis, C. R., Commault, A. S., Ralph, P. J., & Tamburic, B. (2016) · Algal Research · doi:10.1016/j.algal.2016.07.009 · Cited by 53 · see citing works

Authors: Wijffels, R. H., & Barbosa, M. J.
Affiliations: Wageningen University & Research (NL)
Wageningen University and Research Center, Food and Biobased Research, Post Office Box 17, 6700 AA Wageningen, Netherlands.
ORCID: Wijffels, René H.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2010-08-13
Volume: 329
Issue: 5993
Pages: 796-799
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1189003
OpenAlex: W2002547950
Cited by: 1,886
References: 26
Citations by year: 2026: 59 · 2025: 63 · 2024: 65 · 2023: 69 · 2022: 82 · 2021: 96 · 2020: 146 · 2019: 138 · 2018: 136 · 2017: 153 · 2016: 135 · 2015: 168 · 2014: 162 · 2013: 175 · 2012: 139
FWCI: 61.61
Type: journal article
Language: en
Open access: green
research.wur.nl
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Socio-Environmental Systems Modeling (acceptedVersion)
Socio-Environmental Systems Modeling (submittedVersion)
Data Archiving and Networked Services (DANS) (submittedVersion)
Topics: Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords: microalgae, algal biofuels, biodiesel, algal lipid production, CO2 fixation, photosynthetic microorganisms, oleaginous crops, systems biology, genetic engineering, biorefining, industrial-scale production
MeSH terms: Biotechnology, Carbon Dioxide, Genetic Engineering, Lipids, Sunlight, Technology, Water, Biomass, Bioreactors, Lipid Metabolism, Biofuels, Eukaryota

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