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Cited by 49Reproductive tract infections researchProbiotics and Fermented FoodsReproductive Health and Technologies

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Pregnancy outcomes after vaginal probiotic supplementation before frozen embryo transfer: a randomized controlled study

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Thanaboonyawat, Isarin, Pothisan, Sootthinan, Petyim, Somsin, Laokirkkiat, Pitak·2023·Scientific Reports
DOI 10.1038/s41598-023-39078-6PMC PMC10363539
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Abstract

In women receiving assisted reproductive treatment, intrauterine lactobacilli dominance has been associated with higher rates of pregnancy achievement. This randomized controlled trial conducted in the fertility clinic of the university hospital from 7 August 2019 to May 2021, aimed to compare the clinical outcome of embryo transfer in frozen-thaw cycles with Lactobacillus supplementation prior to embryo transfer and the standard treatment. A total of 340 infertile women underwent randomization. The biochemical and clinical pregnancy rates were comparable between the groups (39.9 and 34.2% in the study group vs. 41.8 and 31.7% in the control group); however, the miscarriage rate was significantly decreased in the study group (9.5 vs. 19.1%, respectively, p = 0.02), [OR = 0.44, 95% CI (0.23, 0.86)]. Among 49 women diagnosed with bacterial vaginosis, the live birth rate in the study group was higher than the control group (42.31 vs. 26.09%, p = 0.23), [OR = 2.08, 95% CI (0.62, 6.99)]. In the blastocyst transfer group (n = 206), the live birth rate was significantly higher in the study group than in the control group (35.71 vs. 22.22%, p = 0.03) [OR = 1.9, 95% CI (1.05, 3.59)]. Therefore, intravaginal lactobacilli supplementation before embryo transfer in the frozen-thaw cycle did not improve the biochemical and clinical pregnancy rate in the general population but significantly reduced the miscarriage rate. Trial Registration: TCTR20190429001 (29/04/2019) @ www.thaiclinicaltrials.org .

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Metadata

Authors
Thanaboonyawat, I., Pothisan, S., Petyim, S., & Laokirkkiat, P.
Affiliations
Siriraj Hospital (TH)
Corresponding author
Thanaboonyawat, Isarin
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2023-07-23
Volume
13
Issue
1
Pages
11892-11892
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-023-39078-6
PMC
PMC10363539
OpenAlex
W4385117418
Cited by
49
References
4
Citations by year
2026: 17 · 2025: 19 · 2024: 11 · 2023: 2
FWCI
9.00
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Faculty of Medicine Siriraj Hospital, Mahidol University (R016233020)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Medicine › Reproductive Medicine › Reproductive Health and Technologies
Keywords
vaginal probiotics, frozen embryo transfer, miscarriage rate, bacterial vaginosis, live birth rate, blastocyst transfer, assisted reproduction, infertility, pregnancy outcomes
MeSH terms
Abortion, Spontaneous, Embryo Transfer, Female, Fertilization in Vitro, Humans, Infertility, Female, Pregnancy, Pregnancy Outcome, Retrospective Studies, Pregnancy Rate, Dietary Supplements, Live Birth

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