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Cited by 5Liver physiology and pathologyOrgan Transplantation Techniques and OutcomesTissue Engineering and Regenerative Medicine

Biochemical and morphological responses to post-hepatectomy liver failure in rats

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Lund, Andrea, Andersen, Kasper Jarlhelt, Meier, Michelle, Pedersen, Marie Ingemann, Knudsen, Anders Riegels, Kirkegård, Jakob, Mortensen, Frank Viborg, Nyengaard, Jens Randel·2023·Scientific Reports
DOI 10.1038/s41598-023-40736-yPMC PMC10439910
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Abstract

The upper limit for partial hepatectomy (PH) in rats is 90%, which is associated with an increased risk of post-hepatectomy liver failure (PHLF), correlating with high mortality. Sixty-eight rats were randomized to 90% PH, sham operation, or no surgery. Further block randomization was performed to determine the time of euthanasia, whether 12, 24, or 48 h after surgery. A general distress score (GDS) was calculated to distinguish between rats with reversible (GDS < 10) and irreversible PHLF (GDS ≥ 10). At euthanasia, the liver remnant and blood were collected. Liver-specific biochemistry and regeneration ratio were measured. Hepatocyte proliferation and volume were estimated using stereological methods. All rats subjected to 90% experienced biochemical PHLF. The biochemical and morphological liver responses did not differ between the groups until 48 h after surgery. At 48 h, liver regeneration and function were significantly improved in survivors. The peak mean regeneration ratio was 15% for rats with irreversible PHLF compared to 26% for rats with reversible PHLF. The 90% PH rat model was associated with PHLF and high mortality. Irreversible PHLF was characterized by impaired liver regeneration capacity and an insufficient ability to metabolize ammonia.

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Crossref · 10.1038/s41598-023-40736-y

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  • In experimental models, recovery of liver function has been characterized using stereological and immunohistochemical methods, including assessment of hepatocyte proliferation via Ki-67 staining and β-catenin signaling.

    “In experimental models, the recovery of liver function has been further characterized using stereological and immunohistochemical methods, including assessment of hepatocyte proliferation via Ki-67 staining and β-catenin signaling[92].”

    From Liver regeneration after partial hepatectomy: Triggers and mechanisms (2025) · cites this paper as [92]

    The Materials and methods section (Stereology; page unavailable) describes immunohistochemical staining and stereological counts of Ki-67-positive hepatocytes in rats after hepatectomy. It identifies β-catenin staining as a hepatocyte cell-surface marker for volume measurements, not an assessment of β-catenin signaling. The signaling part of the claim is therefore unsupported. (LLM, conf=0.90)

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Cited by

Sorted by citations

Authors
Lund, A., Andersen, K. J., Meier, M., Pedersen, M. I., Knudsen, A. R., Kirkegård, J., Mortensen, F. V., & Nyengaard, J. R.
Affiliations
Aarhus University (DK)
Corresponding author
Lund, Andrea
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2023-08-19
Volume
13
Issue
1
Pages
13544-13544
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-023-40736-y
PMC
PMC10439910
OpenAlex
W4386002082
Cited by
5
References
1
Citations by year
2026: 1 · 2025: 3 · 2024: 1
FWCI
0.85
Type
journal article
Language
en
Update notices
correction (2024-09-26)
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Arvid Nilssons Fond
  • Købmand Sven Hansen og Hustru Ina Hansens Fond
  • Fabrikant Einar Willumsens Mindelegat
  • Familien Hede Nielsens Fond
  • P. A. Messerschmidt og Hustrus Fond
  • Fonden til Lægevidenskabens Fremme
Topics
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Health Sciences › Medicine › Surgery › Organ Transplantation Techniques and Outcomes
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
post-hepatectomy liver failure, partial hepatectomy, liver regeneration, hepatocyte proliferation, ammonia metabolism, remnant liver, hepatocyte volume, rat model
MeSH terms
Animals, Hepatectomy, Liver Regeneration, Liver Failure, Hepatic Insufficiency, Rats

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