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Cited by 4Helicobacter pylori-related gastroenterology studiesDNA Repair MechanismsGenetic factors in colorectal cancer

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Negative feedback loop in the activation of non-homologous end joining DNA repair pathway in Helicobacter pylori infected patients with gastritis

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Amirnorouzi, Mohsen, Karimi, Ahmadmoeen, Daryani, Naser Ebrahimi, Rajaei, Amiratabak, Hashemi, Mehrdad, Alebouyeh, Masoud·2024·Scientific Reports
DOI 10.1038/s41598-024-74138-5PMC PMC11458588

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Abstract

This study aimed to investigate the activation of error-prone DNA repair pathway in response to Helicobacter pylori infection. Relative changes in the expression levels of genes involved in the non-homologous end-joining pathway (NHEJ) in H. pylori-infected (Cases) and non-infected patients (Controls) with chronic gastritis were measured. A significant increase in the relative expression level of TP53, and significant decrease in the relative transcription of lncRNA LINP1 and XRCC5 were detected in the case group. The transcription of Lig4 and XRCC6 was increased in the case group, which was not statistically significant. The Spearman's Correlation Coefficient analysis showed a significant positive-correlation between the transcriptional levels of LINP1 and XRCC4/XRCC5/Lig4, and between XRCC5 and TP53/Lig4 both in the case and control groups. Moreover, a significant positive correlation between LinP1 and XRCC6 in the case, and a significant positive correlation between XRCC4 and Lig4, and a negative correlation between TP53 and LinP1/XRCC4/XRCC5 in the control group was detected. Although a relative difference was detected in transcriptional levels of the NHEJ gene mediators, downregulation of LinP1 in H. pylori-infected patients proposed the activation of a negative feedback loop, which may interfere with the NHEJ activity at the early stages of gastritis.

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Fig. 1 Gene expression ratios of genes linked to the nonhomologous end joining (NHEJ) DNA repair pathway and related long non-coding RNA, LINP1, in the Helicobacter pyori-infected and non-infected patients. The expression ratios were calculated using the Pfaffl Method. GraphPad Prism, version 10.2.3 was used to do unpaired t test and nonparametric test (Mann–Whitney test) to detect differentially expressed genes between the two groups (ns, > 0.05, *p-value ≤ 0.05, ** p-value ≤ 0.01 and **** p-value ≤ 0.0001). Area under the ROC curve is presented for each gene for H. pyori infected patients.
Fig. 1 Gene expression ratios of genes linked to the nonhomologous end joining (NHEJ) DNA repair pathway and related long non-coding RNA, LINP1, in the Helicobacter pyori-infected and non-infected patients. The expression ratios were calculated using the Pfaffl Method. GraphPad Prism, version 10.2.3 was used to do unpaired t test and nonparametric test (Mann–Whitney test) to detect differentially expressed genes between the two groups (ns, > 0.05, *p-value ≤ 0.05, ** p-value ≤ 0.01 and **** p-value ≤ 0.0001). Area under the ROC curve is presented for each gene for H. pyori infected patients.CC BY-NC-ND · PubMed Central
Fig. 2 The Spearman’s correlation coefficient analysis between the H. pylori infected and non-infected patients with chronic gastritis for study of diversity in the transcription of gene mediators involving in non-homologous end joining DNA repair system.a
Fig. 2 The Spearman’s correlation coefficient analysis between the H. pylori infected and non-infected patients with chronic gastritis for study of diversity in the transcription of gene mediators involving in non-homologous end joining DNA repair system.aCC BY-NC-ND · PubMed Central

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Metadata

Authors
Amirnorouzi, M., Karimi, A., Daryani, N. E., Rajaei, A., Hashemi, M., & Alebouyeh, M.
Affiliations
  • Islamic Azad University, Tehran (IR)
  • Shahid Beheshti University (IR)
  • Imam Khomeini Hospital (IR)
  • University of South Florida (US)
Corresponding author
Alebouyeh, Masoud
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2024-10-07
Volume
14
Issue
1
Pages
23363-23363
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-024-74138-5
PMC
PMC11458588
OpenAlex
W4403177453
Cited by
4
References
1
Citations by year
2026: 2 · 2025: 2
FWCI
1.68
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by-nc-nd)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran (32897)
Topics
  • Health Sciences › Medicine › Gastroenterology › Helicobacter pylori-related gastroenterology studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
Keywords
Helicobacter pylori infection, chronic gastritis, non-homologous end joining, DNA repair, negative feedback loop, TP53, long non-coding RNA, Ku80, XRCC4, Ku70, LIG4, gene expression
MeSH terms
Ku Autoantigen, DNA Ligase ATP, Adult, DNA-Binding Proteins, Female, Gastritis, Humans, Male, Middle Aged, Tumor Suppressor Protein p53, Helicobacter pylori, Helicobacter Infections, Feedback, Physiological, DNA End-Joining Repair, RNA, Long Noncoding

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