Extracellular vesicles of minimalistic Mollicutes as mediators of immune modulation and horizontal gene transfer
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Abstract
Extracellular vesicles (EVs) are central components of bacterial secretomes, including the small, cell wall-less Mollicutes. Although EV release in Mollicutes has been reported, EV proteomic composition and function have not been explored yet. We developed a protocol for isolating EVs of the pathogens Mycoplasma mycoides subsp. capri (Mmc) and Mycoplasma (Mycoplasmopsis) bovis and examined their functionality. Proteomic analysis demonstrated that EVs mirror the proteome of the EV-producing bacteria. EVs exhibited nuclease activity, effectively digesting both circular and linear DNA. Notably, M. bovis EVs elicited immune responses in bovine primary blood cells, like those induced by live M. bovis. Our findings reveal that EVs can carry plasmids and enable their horizontal transfer, known as vesiduction. Specifically, the natural plasmid pKMK1, with an unknown transmission route, was detected in EVs of Mmc 152/93 and the tetM-containing pIVB08 plasmid was associated with EVs released by an Mmc GM12 strain carrying this plasmid. pIVB08 could be transferred via homo- and heterologous vesiduction to Mmc, M. capricolum subsp. capricolum and M. leachii. Vesiduction was impeded by membrane disruption but resisted DNase and Proteinase K treatment, suggesting that EVs protect their cargo. These findings enhance our understanding of Mollicutes EVs, particularly in host interactions and horizontal gene transfer.
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- Authors
- Wagner, T. M., Torres-Puig, S., Yimthin, T., Irobalieva, R. N., Heller, M., Kaessmeyer, S., Démoulins, T., & Jores, J.
- Affiliations
- University of Bern (CH)
- Corresponding author
- Wagner, Theresa Maria
- ORCID
- Venue
- Communications Biology
- Published
- 2025-04-29
- Volume
- 8
- Issue
- 1
- Pages
- 674-674
- ISSN
- 2399-3642 (ISSN-L)
- PMC
- PMC12041197
- OpenAlex
- W4409958832
- Cited by
- 14
- References
- 4
- Citations by year
- 2026: 7 · 2025: 7
- FWCI
- 4.21
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Communications Biology (publishedVersion, cc-by-nc-nd)
- Research Square (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Open Access CRIS of the University of Bern
- Funders
- Swiss National Science Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Keywords
- extracellular vesicles, Mollicutes, Mycoplasma mycoides subsp. capri, Mycoplasma bovis, horizontal gene transfer, plasmid transfer, nuclease activity, immunomodulation, tetM, vesicle proteome, host-pathogen interaction
- MeSH terms
- Extracellular Vesicles, Animals, Cattle, Tenericutes, Mycoplasma, Mycoplasma mycoides, Plasmids, Gene Transfer, Horizontal, Proteomics, Immunomodulation
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