A tuneable minimal cell membrane reveals that two lipid species suffice for life

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Ye, T., Sun, Y., Chai, Y., Duma, L., & Rossez, Y. (2025) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2025.159630 · Cited by 4 · see citing works

How receptor conformation depends on lipid nanodisc size: Adenosine A2A receptor and implications for class-A GPCR proteins

Hägg, V., Levental, I., Kaptan, S., & Vattulainen, I. (2026) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2026.184535 · Cited by 2 · see citing works

Contribution to the special BBA issue dedicated to Joachim Seelig – From lipid bilayers to the innate immune system

Seelig, A. (2025) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2025.184483 · Cited by 1 · see citing works

Autonomous Synthesis and Scrambling of Phospholipids, Linked to Recycling of Cofactors in Synthetic Cells

Coenradij, J., Bailoni, E., Lupacchini, M., Groenhof, W. F., Venekamp, M., Slotboom, D. J., Driessen, A. J. M., Exterkate, M., & Poolman, B. (2026) · ACS Synthetic Biology · doi:10.1021/acssynbio.5c00973 · Cited by 1 · see citing works

A novel expression system for imaging single-molecule fluorescence in Haloferax volcanii WR806 enables visualization of altered Cas1 dynamics during UV-induced DNA damage response

Schrage, P. R., Afonina, U., Wörtz, J., Marchfelder, A., Martens, K. J. A., Sáenz, J., & Endesfelder, U. (2026) · microLife · doi:10.1093/femsml/uqag014 · Cited by 1 · see citing works

High-yield microbial lipid synthesis through organic waste co-fermentation guided by reverse carbon and nitrogen regulation: Optical photothermal infrared and transcriptomic insights

Sun, S., Cao, H., Chandran, K., Cheng, S., Wang, X., & Li, Z. (2026) · Bioresource Technology · doi:10.1016/j.biortech.2026.135182 · Cited by 1 · see citing works

Chemically defined lipid diets reveal the versatility of lipidome remodeling in genomically minimal cellsPreprint

Safronova, N., Junghans, L., Oertel, J., Fahmy, K., & Sáenz, J. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.10.04.616688 · Cited by 1 · see citing works

Unraveling the Transcriptional Landscape within a Minimized Bacterium via Comparative AnalysisPreprint

Brier, T. A., Cournoyer, J. E., Gilbert, B. R., Glass, S. A., Gao, Y.-L., Yu, Y., Thornburg, Z. R., Goglin, K. C., John, G., Mamaghani, T., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.10.23.681674 · Cited by 1 · see citing works

From hot to cold: dissecting lipidome adaptation in Mycoplasma mycoides and the Minimal Cell JCVI-Syn3BPreprint

Safronova, N., Junghans, L., & Sáenz, J. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.11.10.566608

Sterol-lipids enable large-scale, liquid-liquid phase separation in bilayer membranes of only 2 componentsPreprint

Wilson, K. J., Nguyen, H. Q., Gervay‐Hague, J., & Keller, S. L. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.02.02.578692

A novel expression system for imaging single-molecule fluorescence in Haloferax volcanii WR806 enables visualization of Cas1 involvement in UV-light associated DNA repairPreprint

Schrage, P. R., Afonina, U., Wörtz, J., Marchfelder, A., Martens, K. J. A., Sáenz, J., & Endesfelder, U. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.02.24.639823

Do model membranes still matter?

Gbadamosi, O., & Marquardt, D. (2025) · Advances in biomembranes and lipid self-assembly · doi:10.1016/bs.abl.2025.04.001

Nothing can replace polyunsaturated lipids

Rodriguez-Gallardo, S., & 原山, 武. (2025) · eLife · doi:10.7554/elife.108249

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

Gan, S., Scarpelli, V., & Exterkate, M. (2025) · FEBS Letters · doi:10.1002/1873-3468.70131 · Cited by 1 · see citing works

Evo-Inspired Engineering of Radical Phenotypes and Emergent Traits

Babonis, L. S., Hahn, D. A., Liu, A. P., & Widhalm, J. R. (2025) · Integrative and Comparative Biology · doi:10.1093/icb/icaf107

A Robust Method for On-Chip Production and Manipulation of Lipid Vesicles by Inverted EmulsionPreprint

Yandrapalli, N., Gonzalez, D., Leng, W., Alsayyah, C., Ernst, R., & Tang, T. ‐. D. (2025) · SSRN Electronic Journal · doi:10.2139/ssrn.5392350

Metabolic heat flow from the minimal cell JCVI-Syn3B reveals the lipidome-dependence of growth and metabolismPreprint

Oertel, J., Safronova, N., Sáenz, J., & Fahmy, K. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.09.12.675810

Breaking the acid barrier: Unveiling the metabolic response mechanisms of oleaginous yeasts to volatile fatty acids using novel optical photothermal infrared technology

Sun, S., Yu, Q., Wang, X., Lu, D., Cao, H., Cheng, S., & Li, Z. (2025) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2025.108587

De novo chemoenzymatic construction of lipid membranes

Khanal, S., Harjung, A., & Fracassi, A. (2026) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2026.01.013

A robust method for on-chip production and manipulation of lipid vesicles by inverted emulsion

Yandrapalli, N., Gonzales, D. T., Leng, W., Alsayyah, C., Abdukarimov, N., Ernst, R., & Tang, T. ‐. D. (2026) · Cell Reports Methods · doi:10.1016/j.crmeth.2026.101326

A cationic single-chain bolalipid forms stable vesicles with distinct interfacial behavior

Gahtori, P., Varghaei, H., Hazra, B., Jankovic, C. L., Strobl, J., Kaur, H., Khamis, S., West, F. G., Gibbs, J. M., & Mansy, S. S. (2026) · Biophysical Journal · doi:10.1016/j.bpj.2026.03.044

Collective lipid dynamics in biomembranes

Brown, M. F. (2026) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2026.184553

Beyond traditional PFAS: Potent neurotoxicity of 6:2 Cl-PFESA through lipidomic dysregulation

Lin, R.-X., Ding, Y.-Y., Cao, S.-X., Qing-yuan, D., Luo, X., Shi, Y.-L., Duan, W.-J., Li, Y.-F., Sun, W., He, R.-R., et al. (2026) · Emerging contaminants · doi:10.1016/j.emcon.2026.100712

Functional Roles of Individual Membrane Phospholipids in Escherichia coli and Saccharomyces cerevisiae

Bogdanov, M. V., Dowhan, W., Mileykovskaya, E., Vitrac, H., & Sawasato, K. (2026) · Springer eBooks · doi:10.1007/978-3-319-43676-0_36-2

Hadal trench microbiomes as models of extreme adaptation and ecosystem function

Barcan, A. S., Barcan, R. A., He, L., & Wang, Y. (2026) · Communications Earth & Environment · doi:10.1038/s43247-026-03922-0

Minimal organism JCVI-syn3.0, an engineered derivative of Mycoplasma mycoides , encodes an assortment of unrecognized membrane transporters and immunity factors

Mushegian, A. R., Yamamoto, P. K., Torikai, N., & Arakawa, K. (2026) · mSphere · doi:10.1128/msphere.00077-26

Transcriptomic and Metabolomic Analyses of the Mechanisms Underlying Zearalenone Removal by Lactobacillus paracasei

Gan, M., Chen, P., Zeng, R., Wang, F., & Zhao, Y. (2026) · Toxins · doi:10.3390/toxins18090382

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

Presutti, C., & Poolman, B. (2026) · FEBS Letters · doi:10.1002/1873-3468.70476

Visualizing Mycoplasma with Nanometer Resolution without Compromising Viability

Kumar, A., Perry, N., Paul, A., Özdoğan, M., Joseph, J., Ringnalda, J., Murkute, P., & Timp, G. L. (2026) · ACS Nano · doi:10.1021/acsnano.6c08047

Why cells use many lipids when few will do

Sáenz, J. (2026) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-026-01041-4

Understanding the Design Principles of Living Membranes

Justice, I. (2024) · Qucosa (Saxon State and University Library Dresden)

Chemically defined lipid diets reveal the versatility of lipidome remodeling in genomically minimal cellsPreprint

Safronova, N., Junghans, L., Oertel, J., Fahmy, K., & Sáenz, J. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.10.04.616688 · Cited by 1 · see citing works

Fundamental behaviors emerge from simulations of a living minimal cell

Thornburg, Z. R., Bianchi, D. M., Brier, T. A., Gilbert, B. R., Earnest, E. E., Melo, M. C. R., Safronova, N., Sáenz, J. P., Cook, A. T., Wise, K. S., et al. (2022) · Cell · doi:10.1016/j.cell.2021.12.025 · Cited by 165 · see citing works

BPS2026 – Toward decoding and engineering membrane functions

Sáenz, J. (2026) · Biophysical Journal · doi:10.1016/j.bpj.2025.11.1561

Why cells use many lipids when few will do

Sáenz, J. (2026) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-026-01041-4