The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Oxygen and mammalian cell culture: are we repeating the experiment of Dr. Ox?
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In the citing paper
“In such extreme cases, variations in culture conditions may resemble levels consistent with hypercapnia and hypoxia rather than conditions typical of in vivo extracellular fluids, although in vivo environments vary considerably among selected tissues (Ast and Mootha, 2019).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Ast and Mootha, 2019
In the citing paper
“For instance, O2 levels vary across human tissues and range from 13% O2 in the lung-pulmonary vein to 1–3% O2 in the uterus (Ast and Mootha, 2019).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Ast and Mootha, 2019
In the citing paper
“By considering how niche in vivo environments affect experimental outcomes, researchers could further increase the robustness of their experiments and increase the likelihood that findings have relevance to focal in vivo compartments (Ast and Mootha, 2019).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Ast and Mootha, 2019
In the citing paper
“although reliable reports of environmental parameters within selected human tissues are presently limited (Al-Ani et al., 2018; Ast and Mootha, 2019)”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Ast and Mootha, 2019
Citations
Cites
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Self-citationHistone demethylase KDM6A directly senses oxygen to control chromatin and cell fate
Chakraborty, A. A., Laukka, T., Myllykoski, M., Ringel, A. E., Booker, M. A., Tolstorukov, M. Y., Meng, Y. J., Meier, S. R., Jennings, R. B., Creech, A. L., et al. (2019) · Science · doi:10.1126/science.aaw1026 · Cited by 432
Low oxygen concentration extends the lifespan of cultured human diploid cells
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Hypoxia induces rapid changes to histone methylation and reprograms chromatin
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Nox4: A Hydrogen Peroxide-Generating Oxygen Sensor
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Cited by
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Tan, J., Virtue, S., Norris, D. M., Conway, O. J., Yang, M. H., Gribben, C., Lugtu, F., Kamzolas, I., Krycer, J. R., Mills, R. J., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.11.29.516437 · Cited by 2
On the Intensity of the Microvascular Magnetic Field in Normal State and Septic Shock
Chalkias, A. F. (2025) · Journal of Clinical Medicine · doi:10.3390/jcm14072496 · Cited by 1
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Hydrogen sulfide increases intracellular oxygen and inhibits the HIF response
Brake, J., Hanna, D. A., Kumar, R., Peng, Q., Landry, A. P., Singhal, R., Weerapana, E., Shah, Y. M., & Banerjee, R. V. (2026) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2026.111151 · Cited by 3
Oxygen-sensitive TERT Promoter Methylation Regulates Telomerase ActivityPreprint
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Hydrogen sulfide increases intracellular oxygen and regulates the HIF responsePreprint
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An archaeological survey of Ufe settlements: a preliminary report
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Oxygen matters: Unraveling the role of oxygen in the neuronal response to cisplatin
Crugeiras, J., Calls, A., Contreras, E., Alemany, M., Navarro, X., Yuste, V. J., Casanovas, O., Udina, E., & Bruna, J. (2024) · Journal of the Peripheral Nervous System · doi:10.1111/jns.12659
Repeated hyperbaric oxygen exposure accelerates fatigue and impairs SR-calcium release in mice
Gasier, H. G., Kovach, J., & Porter, K. (2024) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00723.2024
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Multi-species genomic study of oxidative stress response and adaptation
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Severe Hypoxia Reduces Adenylyl Cyclase 6 Expression and Function in Bronchial Epithelial Cells
Cunnington, R. H., Singh, N., Shafizadeh, M., Hanrahan, J. W., Dakshinamurti, S., & Chelikani, P. (2026) · FASEB BioAdvances · doi:10.1096/fba.2025-00309
Microfabrication of cell culture microenvironments with spatially controlled oxygen levels
Becconi, M., Malferrari, M., Lesch, A. S., Salvatore, D., Zironi, I., Conte, M., Simonetti, G., Pelicci, P. G., Girault, H. H., Salvioli, S., et al. (2026) · Device · doi:10.1016/j.device.2026.101146
IncuSense: IoT-Enabled Wireless Long-Term Multiparametric Cell Culture Incubator Monitoring System
Kumar, A., Patel, R. D., Johnson, D., & Jain, A. (2026) · Annals of Biomedical Engineering · doi:10.1007/s10439-026-04284-6
The Role of Oxygen Tension in Modulating Wound Healing Phases
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Metadata
- Authors
- Ast, T., & Mootha, V. K.
- Affiliations
- Broad Institute (US)
- Corresponding author
- Mootha, Vamsi K.
- Venue
- Nature Metabolism
- Published
- 2019-08-05
- Volume
- 1
- Issue
- 9
- Pages
- 858-860
- ISSN
- 2522-5812 (ISSN-L)
- OpenAlex
- W2966730488
- Cited by
- 125
- References
- 20
- Citations by year
- 2026: 7 · 2025: 20 · 2024: 23 · 2023: 13 · 2022: 27 · 2021: 20 · 2020: 13 · 2019: 1
- FWCI
- 4.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Nature Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Health & Human Services | National Institutes of Health (R35GM122455)
- Howard Hughes Medical Institute
- Marriot Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- oxygen, mammalian cell culture, reproducibility, hypoxia, experimental interpretation
- MeSH terms
- Animals, Culture Media, Mammals, Oxygen, Research
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