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Cited by 9Soil Carbon and Nitrogen DynamicsMicrobial metabolism and enzyme functionMethane Hydrates and Related Phenomena

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Interkingdom interaction: the soil isopod Porcellio scaber stimulates the methane-driven bacterial and fungal interaction

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Heffner, Tanja, Brami, Semi A, Mendes, Lucas W, Kaupper, Thomas, Hannula, Emilia S, Poehlein, Anja, Horn, Marcus A, Ho, Adrian·2023·ISME Communications
DOI 10.1038/s43705-023-00271-3PMC PMC10290665
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Abstract

Porcellio scaber (woodlice) are (sub-)surface-dwelling isopods, widely recognized as “soil bioengineers”, modifying the edaphic properties of their habitat, and affecting carbon and nitrogen mineralization that leads to greenhouse gas emissions. Yet, the impact of soil isopods on methane-cycling processes remains unknown. Using P. scaber as a model macroinvertebrate in a microcosm study, we determined how the isopod influences methane uptake and the associated interaction network in an agricultural soil. Stable isotope probing (SIP) with 13C-methane was combined to a co-occurrence network analysis to directly link activity to the methane-oxidizing community (bacteria and fungus) involved in the trophic interaction. Compared to microcosms without the isopod, P. scaber significantly induced methane uptake, associated to a more complex bacteria-bacteria and bacteria-fungi interaction, and modified the soil nutritional status. Interestingly, 13C was transferred via the methanotrophs into the fungi, concomitant to significantly higher fungal abundance in the P. scaber-impacted soil, indicating that the fungal community utilized methane-derived substrates in the food web along with bacteria. Taken together, results showed the relevance of P. scaber in modulating methanotrophic activity with implications for bacteria-fungus interaction.

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Metadata

Authors
Heffner, T., Brami, S. A., Mendes, L. W., Kaupper, T., Hannula, E. S., Poehlein, A., Horn, M. A., & Ho, A.
Affiliations
  • Leibniz University Hannover (DE)
  • Universidade de São Paulo (BR)
  • Leiden University, Department of Environmental Biology, Institute of Environmental Sciences , Einsteinweg 2, 2333CC Leiden, the Netherlands
  • University of Göttingen (DE)
Corresponding author
  • Horn, Marcus A
  • Ho, Adrian
ORCID
Venue
ISME Communications
Publisher
Oxford University Press (OUP)
Published
2023-06-24
Volume
3
Issue
1
Pages
62-62
ISSN
2730-6151 (ISSN-L)
DOI
10.1038/s43705-023-00271-3
PMC
PMC10290665
OpenAlex
W4381929523
Cited by
9
References
88
Citations by year
2026: 2 · 2025: 4 · 2024: 3
FWCI
1.34
Type
journal article
Language
en
Open access
Licence
Locations
  • ISME Communications (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Leiden Repository (Leiden University) (submittedVersion, cc-by)
  • Institutional Repository of Leibniz Universität Hannover (Leibniz Universität Hannover) (submittedVersion, cc-by)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
  • Institutional Repository of Leibniz Universität Hannover (Leibniz Universität Hannover) (submittedVersion, cc-by-nc-nd)
  • Leibniz Universität Hannover (cc-by)
Funders
Deutsche Forschungsgemeinschaft (HO6234/1-2)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil Carbon and Nitrogen Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
  • Physical Sciences › Environmental Science › Environmental Chemistry › Methane Hydrates and Related Phenomena
Keywords
Porcellio scaber, terrestrial isopods, methane uptake, methanotrophic bacteria, fungal-bacterial interactions, soil microbial communities, soil bioengineering, trophic interactions, co-occurrence network analysis, agricultural soils, soil carbon cycling, greenhouse gas emissions

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