Type distribution, viral load and integration status of high-risk human papillomaviruses in pre-stages of cervical cancer (CIN)
Open accessAbstract
A series of 176 archival cervical intraepithelial neoplasia (CIN) was analysed for the presence, viral load and integration status of 'high-risk' types of human papillomavirus (HR-HPV). The samples were assayed using newly developed methods based on real-time PCR. Two methods for the extraction of DNA from the paraffin-embedded biopsies were compared: a protocol based on the MagNA pure system (Roche) and a Qiagen spin column kit (Qiagen). It was possible to amplify 94% (166) of the samples. Of these, 36, 63 and 80% of the CIN I, II and III cases contained HR-HPV. HPV 16 was the most prevalent, and was found in 20, 28 and 46% of the CIN I, II and III cases, respectively. The second most frequent HR-HPV was type 33 group, and in CIN II it was as prevalent as HPV 16. The median number of copies of HR-HPV per cell was not significantly different in the CIN I, II and III cases, but there was a wide range of viral load values over several magnitudes, regardless of the grade of CIN. All samples were found to contain integrated forms of HPV 16, frequently mixed with an episomal form.
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Other studies have not found a correlation between increased HR-HPV viral load and the severity and progression of cervical lesions.
“while others have not found such a correlation [15,16].”
From Comparative evaluation of Allplex HPV28 and Anyplex II HPV28 assays for high-risk HPV genotyping in cervical samples (2025) · cites this paper as [16]
The prognostic significance of HR-HPV viral load in cervical cancer remains controversial.
“The prognostic significance of HR-HPV viral load in cervical cancer remains controversial.”
From Comparative evaluation of Allplex HPV28 and Anyplex II HPV28 assays for high-risk HPV genotyping in cervical samples (2025) · cites this paper as [16]
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Mafi, S., Theuillon, F., Meyer, S., Woillard, J.-B., Dupont, M., Rogez, S., Alain, S., & Hantz, S. (2025) · PLOS ONE · doi:10.1371/journal.pone.0320978 · Cited by 9 · see citing works
“Previous studies have reported an association between increased HR-HPV viral load and the severity and progression of cervical lesions [13,14], while others have not found such a correlation [15,16].”
- Authors
- Andersson, S., Safari, H., Mints, M., Lewensohn- Fuchs, I., Gyllensten, U., & Johansson, B.
- Affiliations
- Karolinska University Hospital (SE)
- Uppsala University (SE)
- Corresponding author
- Andersson, S
- Venue
- British Journal of Cancer
- Published
- 2005-06
- Volume
- 92
- Issue
- 12
- Pages
- 2195-2200
- ISSN
- 0007-0920 (ISSN-L)
- 1532-1827
- PMC
- PMC2361820
- OpenAlex
- W2040572148
- Cited by
- 134
- References
- 0
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 7 · 2018: 4 · 2017: 5 · 2016: 8 · 2015: 5 · 2014: 7 · 2013: 3 · 2012: 7
- FWCI
- 9.37
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-sa
- www.springer.com/tdm (unspecified)
- Locations
- British Journal of Cancer (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Repositorio Institucional Universidad de Sucre (submittedVersion, cc-by-nc)
- Americanae (AECID Library) (submittedVersion, cc-by-nc)
- Topics
- Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
- Health Sciences › Medicine › Otorhinolaryngology › Head and Neck Cancer Studies
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- high-risk HPV, cervical intraepithelial neoplasia, HPV viral load, viral integration, HPV16, HPV 33, HPV genotyping, episomal HPV, cervical cancer precursors
- MeSH terms
- Uterine Cervical Dysplasia, Uterine Cervical Neoplasms, DNA, Viral, Female, Humans, Viral Load, Papillomaviridae, Papillomavirus Infections
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