Mechanisms of AVP‐induced glucagon release in clonal α‐cells In‐R1‐G9: involvement of Ca2+‐dependent and ‐independent pathways
Open accessAbstract
The mechanisms underlying AVP‐induced increase in [Ca 2+]i and glucagon release in clonal α‐cells In‐R1‐G9 were investigated. AVP increased [Ca 2+]i and glucagon release in a concentration‐dependent manner. After the administration of AVP, glucagon was released within 30 s, quickly reached the maximum within 2 min, and maintained a steady‐state concentration for at least 15 min. In Ca 2+‐containing medium, AVP increased [Ca 2+]i in a biphasic pattern; a peak followed by a sustained plateau. In Ca 2+‐free medium, the Ca 2+ response to AVP became monophasic with lower amplitude and no plateau. Both the basal and AVP‐induced glucagon releases were lower in the absence than in the presence of extracellular Ca 2+. When [Ca 2+]i was stringently deprived by BAPTA, a Ca 2+ chelator, AVP still significantly increased glucagon release. Pretreatment with thapsigargin, a microsomal Ca 2+ ATPase inhibitor, abolished both the Ca 2+ peak and sustained plateau. AVP increased intracellular concentration of IP 3. U‐73122 (8 μ M), a phospholipase C inhibitor, abolished AVP‐induced increases in [Ca 2+]i, but only reduced AVP‐induced glucagon release by 39%. Pretreatment with nimodipine, an L‐type Ca 2+ channel blocker failed to alter AVP‐induced glucagon release or increase in [Ca 2+]i. The results suggest that AVP causes glucagon release through both Ca 2+‐dependent and ‐independent pathways. For the Ca 2+‐dependent pathway, the G q protein activates phospholipase C, which catalyzes the formation of IP 3. IP 3 induces Ca 2+ release from the endoplasmic reticulum, which, in turn, triggers Ca 2+ influx. Both Ca 2+ release and Ca 2+ influx may contribute to AVP‐induced glucagon release. British Journal of Pharmacology (2000) 129, 257–264; doi:10.1038/sj.bjp.0703037
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Vasopressin stimulates gluconeogenesis and glycogenolysis through hepatic V1a receptors.
“Ex vivo studies showed that VP stimulates gluconeogenesis and glycogenolysis through the activation of hepatic arginine VP receptor 1A (V1aR)”
From Vasopressin and hydration play a major role in the development of glucose intolerance and hepatic steatosis in obese rats (2015) · cites this paper as [8]
Vasopressin stimulates release of glucagon or insulin from pancreatic islets through V1b receptors, depending on extracellular glucose.
“and the release of either glucagon or insulin, depending on concomitant extracellular glucose levels, through the activation of arginine vasopressin receptor 1B (V1bR) in pancreatic islets [7][8][9].”
From Vasopressin and hydration play a major role in the development of glucose intolerance and hepatic steatosis in obese rats (2015) · cites this paper as [8]
Cites
Thastrup, M., Cullen, P. J., Drøbak, B. K., Hanley, M. R., & Dawson, A. P. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.7.2466 · Cited by 3,272 · see citing works
Bleasdale, J. E., Thakur, N. R., Gremban, R. S., Bundy, G. L., Fitzpatrick, F. A., Smith, R. J., & Bunting, S. (1990) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)22967-6 · Cited by 629 · see citing works
Thompson, A. K., Mostafapour, S. P., Denlinger, L. C., et al. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54362-3 · Cited by 449 · see citing works
Yule, D. I., & Williams, J. A. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)49643-9 · Cited by 300 · see citing works
Jard, S., Gaillard, R. C., Guillon, G., Marie, J., Schoenenberg, P., Muller, A. F., Manning, M., & Sawyer, W. H. (1986) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)10336-2 · Cited by 276 · see citing works
Calcium and T lymphocyte activation
Gardner, P. I. (1989) · Cell · doi:10.1016/0092-8674(89)90865-9 · Cited by 242 · see citing works
Rhythmic Exocytosis Stimulated by GnRH-Induced Calcium Oscillations in Rat Gonadotropes
Tse, A., Tse, F. W., Almers, W., & Hille, B. (1993) · Science · doi:10.1126/science.8385366 · Cited by 239 · see citing works
Correlation between cytosolic free Ca2+ and insulin release in an insulin-secreting cell line.
Wollheim, C. B., & Pozzan, T. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)43347-3 · Cited by 200 · see citing works
Gromada, J., Bokvist, K., Ding, W.-G., Barg, S., Buschard, K., Renström, E., & Rorsman, P. (1997) · The Journal of General Physiology · doi:10.1085/jgp.110.3.217 · Cited by 196 · see citing works
Russell, J. T., Brownstein, M., & Gainer, H. (1980) · Endocrinology · doi:10.1210/endo-107-6-1880 · Cited by 149 · see citing works
Hsu, W. H., Xiang, H. D., Rajan, A. S., Kunze, D. L., & BOYD, A. E. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)35249-3 · Cited by 140 · see citing works
Self-citationRichards, J. S., Jonassen, J. A., ROLFES, A. I., et al. (1979) · Endocrinology · doi:10.1210/endo-104-3-765 · Cited by 137 · see citing works
Thibonnier, M., Preston, J. A., Dulin, N. O., Wilkins, P. L., Berti‐Mattera, L. N., & Mattera, R. (1997) · Endocrinology · doi:10.1210/endo.138.10.5432 · Cited by 135 · see citing works
Signal transduction of V1-vascular vasopressin receptors
Thibonnier, M. (1992) · Regulatory Peptides · doi:10.1016/0167-0115(92)90067-5 · Cited by 132 · see citing works
Smallridge, R. C., Kiang, J. G., Gist, I. D., et al. (1992) · Endocrinology · doi:10.1210/endo.131.4.1396332 · Cited by 126 · see citing works
Isolation of glucagon-secreting cell lines by cloning insulinoma cells
Takaki, R., Ono, J., Nakamura, M., Yokogawa, Y., Kumae, S., Hiraoka, T., Yamaguchi, K., Hamaguchi, K., & Uchida, S. (1986) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/bf02623498 · Cited by 107 · see citing works
Mannose 6-phosphate-independent targeting of cathepsin D to lysosomes in HepG2 cells.
Rijnboutt, S., Kal, A. J., Geuze, H. J., et al. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54323-4 · Cited by 107 · see citing works
Kirk, C. J., Rodrigues, L. M., & HEMS, D. A. (1979) · Biochemical Journal · doi:10.1042/bj1780493 · Cited by 94 · see citing works
Dunning, B. E., Moltz, J. H., & Fawcett, C. P. (1984) · Peptides · doi:10.1016/0196-9781(84)90109-8 · Cited by 85 · see citing works
Multiple Signaling Pathways of V1-Vascular Vasopressin Receptors of A75rCells*
Thibonnier, M., Bayer, A. L., Simonson, M. S., et al. (1991) · Endocrinology · doi:10.1210/endo-129-6-2845 · Cited by 83 · see citing works
Metz, S. A., & Dunlop, M. (1990) · Biochemical Journal · doi:10.1042/bj2700427 · Cited by 76 · see citing works
Yoshida, S., & Plant, S. (1992) · The Journal of Physiology · doi:10.1113/jphysiol.1992.sp019419 · Cited by 65 · see citing works
Use of U-73122 as an Inhibitor of Phospholipase C-Dependent Processes
Bleasdale, J. E., & Fisher, S. K. (1993) · Neuroprotocols · doi:10.1006/ncmn.1993.1046 · Cited by 54 · see citing works
Smallridge, R. C. (1992) · Endocrinology · doi:10.1210/en.131.4.1883 · Cited by 54 · see citing works
Spatz, M., Stanimirovic, D. B., Bacic, F., et al. (1994) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1994.266.3.c654 · Cited by 46 · see citing works
Li, G., Pralong, W. F., Pittet, D., et al. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)42842-6 · Cited by 45 · see citing works
Regulation of glucagon release from pancreatic A-cells
Rorsman, P., Ashcrof, F. M., & Berggren, P. (1991) · Biochemical Pharmacology · doi:10.1016/0006-2952(91)90116-m · Cited by 44 · see citing works
Characterization of receptors mediating AVP- and OT-induced glucagon release from the rat pancreas
Yibchok‐anun, S., Cheng, H., Heine, P. A., & Hsu, W. H. (1999) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1999.277.1.e56 · Cited by 42 · see citing works
Self-citationSwope, S. L., & Schönbrunn, A. (1988) · Biochemical Journal · doi:10.1042/bj2530193 · Cited by 42 · see citing works
Wu, H., James-Kracke, M., & Halenda, S. P. (1992) · Biochemistry · doi:10.1021/bi00128a010 · Cited by 36 · see citing works
Cytoplasmic and nuclear signaling pathways of V1-vascular vasopressin receptors
Thibonnier, M., Bayer, A. L., & Leng, Z. (1993) · Regulatory Peptides · doi:10.1016/0167-0115(93)90186-c · Cited by 36 · see citing works
Calcium influx modulates DNA synthesis and proliferation in A7r5 vascular smooth muscle cells
Sperti, G., & Colucci, W. S. (1991) · European Journal of Pharmacology Molecular Pharmacology · doi:10.1016/0922-4106(91)90110-4 · Cited by 36 · see citing works
Vasopressin stimulation of cell proliferation in the rat pituitary gland in vitro
McNicol, A. M., Murray, J. E., & McMeekin, W. (1990) · Journal of Endocrinology · doi:10.1677/joe.0.1260255 · Cited by 33 · see citing works
Hawes, B. E., Waters, S. B., Janovick, J. A., et al. (1992) · Endocrinology · doi:10.1210/endo.130.6.1597151 · Cited by 32 · see citing works
Kimura, M., & Ogihara, M. (1997) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(24)36930-7 · Cited by 27 · see citing works
Yibchok‐anun, S., & Hsu, W. H. (1998) · Life Sciences · doi:10.1016/s0024-3205(98)00463-9 · Cited by 25 · see citing works
Self-citationPhorbol ester and vasopressin activate phospholipase D in leydig cells
Vinggaard, A. M., & Hansen, H. S. (1991) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(91)90106-3 · Cited by 21 · see citing works
Phospholipase A2 expression in human and rodent insulin-secreting cells
Loweth, A. C., Scarpello, J. H. B., & Morgan, N. G. (1995) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(95)03595-x · Cited by 21 · see citing works
Chen, T., Lee, B., & Hsu, W. H. (1994) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)22558-7 · Cited by 20 · see citing works
Self-citationMechanisms of Bradykinin-Induced Insulin Secretion in Clonal beta Cell Line RINm5F
Yang, C., Lee, B., Chen, T.-H., et al. (1997) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(24)36948-4 · Cited by 19 · see citing works
Self-citationFunctional characterization of somatostatin receptors expressed on hamster glucagonoma cells
Fehmann, H. C., Strowski, M. Z., & Göke, B. (1995) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1995.268.1.e40 · Cited by 19 · see citing works
Spontaneous Calcium Oscillations in Clonal Endocrine Pancreatic Glucagon-Secreting Cells
Bode, H., Yule, D. I., Fehmann, H. C., et al. (1994) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1994.2684 · Cited by 17 · see citing works
Lindmar, R., & Löffelholz, K. (1998) · Biochemical Pharmacology · doi:10.1016/s0006-2952(97)00636-9 · Cited by 17 · see citing works
Vasopressin receptor mediated contraction and [3H]inositol metabolism in rat tail artery
Fox, A. W., Friedman, P. A., & Abel, P. W. (1987) · European Journal of Pharmacology · doi:10.1016/0014-2999(87)90751-5 · Cited by 15 · see citing works
Mechanism of vasopressin-induced increase in intracellular Ca2+ in LLC-PK1 porcine kidney cells
Dibas, A. I., Rezazadeh, S. M., Vassan, R., et al. (1997) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1997.272.3.c810 · Cited by 14 · see citing works
Tang, S. H., Yaney, G. C., & Sharp, G. W. G. (1995) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)08636-5 · Cited by 14 · see citing works
Hawes, B. E. (1992) · Endocrinology · doi:10.1210/en.130.6.3475 · Cited by 11 · see citing works
Activation of voltage-sensitive Ca2+ currents by vasopressin in an insulin-secreting cell line
Thorn, P., & Petersen, O. H. (1991) · The Journal of Membrane Biology · doi:10.1007/bf01871365 · Cited by 9 · see citing works
Mediators of Ca2(+)-dependent secretion.
Chaudhry, A., & Rubin, R. N. (1990) · Environmental Health Perspectives · doi:10.1289/ehp.908435 · Cited by 8 · see citing works
Kusaka, I., Ishikawa, S., Higashiyama, M., Saito, T., Nagasaka, S., & Saito, T. (1996) · Endocrinology · doi:10.1210/endo.137.12.8940366 · Cited by 8 · see citing works
Mediators of Ca 2+ -Dependent Secretion
Chaudhry, A., & Rubin, R. P. (1990) · Environmental Health Perspectives · doi:10.2307/3430703 · Cited by 4 · see citing works
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Abu-Basha, E. A., Yibchok-anun, S., & Hsu, W. H. (2002) · Metabolism · doi:10.1053/meta.2002.34052 · Cited by 80 · see citing works
Self-citationFolny, V., Raufaste, D., Lukovic, L., Pouzet, B., Rochard, P., Pascal, M., & Serradeil-Le Gal, C. (2003) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00148.2003 · Cited by 61 · see citing works
- Authors
- Yibchok‐anun, S., Cheng, H., Chen, T., & Hsu, W. H.
- Affiliations
- Iowa State University (US)
- Venue
- British Journal of Pharmacology
- Publisher
- Wiley
- Published
- 2000-01
- Volume
- 129
- Issue
- 2
- Pages
- 257-264
- ISSN
- 0007-1188 (ISSN-L)
- 1476-5381
- PMC
- PMC1571828
- OpenAlex
- W1994602202
- Cited by
- 13
- References
- 51
- Citations by year
- 2020: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2013: 1 · 2012: 1
- FWCI
- 2.16
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- British Journal of Pharmacology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Eli Lilly and Company
- Wayne State University
- Iowa State University
- National Science Council
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- vasopressin, glucagon secretion, alpha cells, intracellular calcium, phospholipase C, IP3, Gq protein, thapsigargin, BAPTA, L-type calcium channels, endoplasmic reticulum calcium release, calcium influx
- MeSH terms
- Cyclic AMP, Amino Acid Chloromethyl Ketones, Animals, Arginine Vasopressin, Calcium, Calcium Channel Blockers, Cell Line, Clone Cells, Enzyme Inhibitors, Estrenes, Glucagon, Cricetinae, Nimodipine, Phosphodiesterase Inhibitors, Pyrrolidinones, Renal Agents, Inositol 1,4,5-Trisphosphate, Serine Proteinase Inhibitors, Thapsigargin, Calcium Channels, L-Type
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