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Cited by 66Plant Virus Research StudiesPlant and Fungal Interactions ResearchRNA Research and Splicing

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A complex network of RNA–RNA interactions controls subgenomic mRNA transcription in a tombusvirus

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Lin, Han‐Xin, White, K Andrew·2004·The EMBO Journal
DOI 10.1038/sj.emboj.7600336PMC PMC514510
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Abstract

Eukaryotic (+)-strand RNA viruses utilize a wide variety of gene expression strategies to achieve regulated production of their viral proteins. A common mechanism used by many is to transcribe viral subgenomic (sg) mRNAs. Transcription of sg mRNA2 in tombusviruses allows for expression of the p19 suppressor of gene silencing and p22 movement proteins. We have investigated the mechanism of transcription of this sg mRNA in Tomato bushy stunt virus and have determined that this process is facilitated by no less than three different RNA modules that are located throughout the viral genome. These RNA units perform distinct tasks and function via long-distance RNA-RNA interactions. Systematic deconstruction of the RNA network and analysis of related RNA promoter elements allowed us to identify fundamental properties necessary for productive sg mRNA2 transcription. Collectively, our results (i) establish specific roles for the different RNA components of a multipartite RNA-based control system, (ii) support a premature termination mechanism for tombusvirus sg mRNA transcription and (iii) reveal a close mechanistic relationship between sg mRNA transcription, viral RNA replication and RNA recombination.

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Metadata

Authors
Lin, H., & White, K. A.
Affiliations
York University (CA)
Corresponding author
White, K Andrew
ORCID
White, K Andrew
Venue
The EMBO Journal
Publisher
Springer Science and Business Media LLC
Published
2004-07-29
Volume
23
Issue
16
Pages
3365-3374
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
DOI
10.1038/sj.emboj.7600336
PMC
PMC514510
OpenAlex
W2136046332
Cited by
66
References
0
Citations by year
2026: 1 · 2024: 3 · 2023: 2 · 2021: 3 · 2020: 2 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 4 · 2013: 5 · 2012: 3
FWCI
6.93
Type
journal article
Language
en
Open access
Licence
Locations
  • The EMBO Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Natural Sciences and Engineering Research Council of Canada
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Virus Research Studies
  • Life Sciences › Immunology and Microbiology › Virology › Plant and Fungal Interactions Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords
tombusvirus, tomato bushy stunt virus, RNA-RNA interactions, early termination, viral RNA replication, RNA recombination, positive-strand RNA viruses
MeSH terms
Base Sequence, Genetic Engineering, Molecular Sequence Data, Promoter Regions, Genetic, RNA, Messenger, RNA, Viral, Transcription, Genetic, Virus Replication, Down-Regulation, Gene Expression Regulation, Viral, Genome, Viral, Tombusvirus, Base Pairing, RNA Stability

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