The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Immune responses limit adenovirally mediated gene expression in the adult mouse eye
Abstract
In order to investigate the immunological consequences of gene transfer to the eye using viral vectors, adenovirus carrying a lacZ reporter gene (AV.LacZ) was injected either subretinally, subconjunctivally or into the anterior chamber of three groups of adult mice: immunocompetent or transiently immunosuppressed BALB/c mice and congenic immunodeficient nude mice. Adenovirally mediated lacZ expression persisted for approximately 3 weeks following injection of the vector into the anterior chamber, retina or extra ocular tissues of the conjuctiva of BALB/c mice. It appears that T cell-mediated immune responses limit the duration of AV-mediated ocular gene expression in adult mice since lacZ gene expression was detected for at least 15 weeks in T cell-deficient BALB/c nude mice, although the level of transgene expression decreased with time. Since intra-ocular AV-mediated gene expression was not significantly longer than extra-ocular expression, it appears that the eye is not normally immune-privileged with respect to viral vectors. Inflammatory cells were detected in the vitreous after anterior chamber injection and in the retina after subretinal injection of adenovirus. The presence of both CD4+ and CD8+ T cells was established by immunophenotyping. Reinjection of BALB/c mice resulted in rapid decline in reporter gene expression, but successful readministration was possible in the case of immunodeficient nude mice. However, after transient depletion of T cells, achieved by intraperitoneal injection of both CD8- and CD4-specific antibodies, the duration of expression in BALB/c mice was longer in the eye (at least 12 weeks, again with decrease in level over time), than in extra-ocular tissues (8 weeks) provided the animal was not reinjected with virus raising the possibility of partial ocular immune-privilege after transient immunosuppression.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In order to investigate the immune consequences of Ad, three groups of adult mice were injected with Ad carrying a lacZ reporter gene into immunocompetent, transiently immunosuppressed BALB/c mice and congenital, immunodeficient nude mice.CD4 + and CD8 + T-cell infiltration was observed in retinal sections, and anti-Ad antibodies were also measured in the immunocompetent mice.The transgene”
From Immune responses to gene editing by viral and non-viral delivery vectors used in retinal gene therapy (2022) · cites this paper as [63]
Citations
Cites
Cellular immune reactivity within the CNS
Wekerle, H., Linington, C., Lassmann, H., & Meyermann, R. (1986) · Trends in Neurosciences · doi:10.1016/0166-2236(86)90077-9 · Cited by 774
Manipulation of Adenovirus Vectors
Graham, F. L., & PREVEC, L. (2003) · Humana Press eBooks · doi:10.1385/0-89603-178-0:109 · Cited by 748
A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells
Zolotukhin, S., Potter, M., Hauswirth, W. W., Guy, J., & Muzyczka, N. (1996) · Journal of Virology · doi:10.1128/jvi.70.7.4646-4654.1996 · Cited by 609
Dai, Y., Schwarz, E. M., Gu, D., Zhang, W., Sarvetnick, N., & Verma, I. M. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.5.1401 · Cited by 590
Monoclonal antibodies to promote marrow engraftment and tissue graft tolerance
Cobbold, S. P., Martín, G., QIN, S., & Waldmann, H. (1986) · Nature · doi:10.1038/323164a0 · Cited by 335
Photoreceptor cell rescue in retinal degeneration (rd) mice by in vivo gene therapy
Bennett, J., Tanabe, T., Sun, D., Zeng, Y., Kjeldbye, H., Gouras, P., & Maguire, A. M. (1996) · Nature Medicine · doi:10.1038/nm0696-649 · Cited by 316
Adenovirus gene transfer causes inflammation in the brain
Byrnes, A. P., Rusby, J. E., Wood, M. J. A., & Charlton, H. M. (1995) · Neuroscience · doi:10.1016/0306-4522(95)00068-t · Cited by 276
Immune Privilege and Immune Regulation in the Eye
Niederkorn, J. Y. (1990) · Advances in immunology · doi:10.1016/s0065-2776(08)60755-5 · Cited by 266
Gene transfer into the mouse retina mediated by an adeno-associated viral vector
Ali, R. R. (1996) · Human Molecular Genetics · doi:10.1093/hmg/5.5.591 · Cited by 258
Self-citationYang, Y., Trinchieri, G., & Wilson, J. M. (1995) · Nature Medicine · doi:10.1038/nm0995-890 · Cited by 243
Cited by
Sorted by citations
Items 101–106 of 106
AAV vectors for retinal gene therapy
Min, S.-H., Dinculescu, A., Glushakova, L. G., & Hauswirth, W. W. (2005) · CRC Press eBooks · doi:10.1201/b13393-51
Rescue of inherited retinal degeneration associated with Aipl1 defects
Ku, C. A. (2012) · The Research Repository @ WVU (West Virginia University) · doi:10.33915/etd.4880
New Experimental Therapeutic Approaches for Degenerative Diseases of the Retina
Tombran‐Tink, J. (2003) · CRC Press eBooks · doi:10.1201/9780203912072.ch18
Gene Therapy for Neovascular Retinopathies
Raisler, B. J., Berns, K. I., & Hauswirth, W. W. (2007) · Humana Press eBooks · doi:10.1007/978-1-59745-047-8_20
Pathophysiology and gene therapy of the optic neuropathy in Wolfram Syndrome
Jagodzinska, J. (2016) · theses.fr (ABES) · doi:10.70675/d5ad003czb010z456bz8738za3feee533351
Gene therapy development for retinal diseases
Reichel, F. F., Bellingrath, J.-S., Király, P., & FISCHER, M. D. (2026) · Handbook of clinical neurology · doi:10.1016/b978-0-443-22212-2.00016-1
Metadata
- Authors
- Reichel, M., Ali, R., Thrasher, A., Hunt, D., Bhattacharya, S., & Baker, D.
- Affiliations
- University College London (GB)
- ORCID
- Baker, D
- Venue
- Gene Therapy
- Published
- 1998-08-01
- Volume
- 5
- Issue
- 8
- Pages
- 1038-1046
- ISSN
- 0969-7128 (ISSN-L)
- 1476-5462
- OpenAlex
- W2047633827
- Cited by
- 106
- References
- 34
- Citations by year
- 2026: 1 · 2025: 1 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 2 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 4
- FWCI
- 4.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Gene Therapy (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Health Sciences › Medicine › Ophthalmology › Retinal Development and Disorders
- Keywords
- adenoviral vector, immune privilege, T cell immunity, subretinal injection, anterior chamber injection, subconjunctival injection, nude mice, immunodeficient mice, transient immunosuppression, CD4+ T cells, CD8+ T cells, vitreous inflammation, ocular immunology
- MeSH terms
- Adenoviridae, Animals, Anterior Chamber, Antibodies, Antibodies, Viral, beta-Galactosidase, Blood-Retinal Barrier, Conjunctiva, Eye, Genetic Vectors, Immunosuppression Therapy, Injections, Retina, T-Lymphocytes, Time Factors, Transfection, Genetic Therapy, Gene Expression, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
