The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Fat content in individual muscle fibers of lean and obese subjects.
Abstract
Objective To examine skeletal muscle intracellular triglyceride concentration in different fiber types in relation to obesity.Design Skeletal muscle fiber type distribution and intracellular lipid content were measured in vastus lateralis samples obtained by needle biopsy from lean and obese individuals.Subjects Seven lean controls (body mass index (BMI) 23.0+/-3.3 kg/m(2); mean+/-s.d.) and 14 obese (BMI 33.7+/-2.7 kg/m(2)) individuals; both groups included comparable proportions of men and women.Measurements Samples were histochemically stained for the identification of muscle fiber types (myosin ATPase) and intracellular lipid aggregates (oil red O dye). The number and size of fat aggregates as well as their concentration within type I, IIA and IIB muscle fiber types were measured. The cellular distribution of the lipid aggregates was also examined.Results The size of fat aggregates was not affected by obesity but the number of lipid droplets within muscle fibers was twice as abundant in obese compared to lean individuals. This was seen in type I (298+/-135 vs 129+/-75; obese vs lean, P<0.05), IIA (132+/-67 vs 79+/-29; P<0.05), and IIB (103+/-63 vs 51+/-13; P<0.05) muscle fibers. A more central distribution of lipid droplets was observed in muscle fibers of obese compared to lean subjects (27.2+/-5.7 vs 19.7+/-6.4%; P<0.05).Conclusion The higher number of lipid aggregates and the disposition to a greater central distribution in all fiber types in obesity indicate important changes in lipid metabolism and/or storage that are fiber type-independent.
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“Although it remains unclear whether any of these defects play a causal role in insulin resistance, intramyocellular lipid excess strongly correlates with the severity of insulin resistance, even after correction for the degree of obesity (94), and has been observed in muscles of multiple fiber types (95).”
From The role of mitochondria in the pathogenesis of type 2 diabetes (2010) · cites this paper as [95]
Citations
Cites
Skeletal Muscle Triglyceride Levels Are Inversely Related to Insulin Action
Pan, D. A., Lillioja, S., Kriketos, A. D., Milner, M. R., Baur, L. A., Bogardus, C., Jenkins, A. B., & Storlien, L. H. (1997) · Diabetes · doi:10.2337/diab.46.6.983 · Cited by 1,179
Skeletal muscle triglyceride levels are inversely related to insulin action
Pan, D. A., Lillioja, S., Kriketos, A. D., Milner, M. R., Baur, L. A., Bogardus, C., Jenkins, A. B., & Storlien, L. H. (1997) · Diabetes · doi:10.2337/diabetes.46.6.983 · Cited by 1,179
Kelley, D. E., Goodpaster, B., Wing, R. R., & Simoneau, J.-A. (1999) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1999.277.6.e1130 · Cited by 1,096
Self-citationStorlien, L. H., Jenkins, A. B., Chisholm, D. J., Pascoe, W. S., Khouri, S., & Kraegen, E. W. (1991) · Diabetes · doi:10.2337/diab.40.2.280 · Cited by 967
Lillioja, S., Young, A. A., Culter, C. L., Ivy, J. L., Abbott, W. G., Zawadzki, J. K., Yki-Järvinen, H., Christin, L., Secomb, T. W., & Bogardus, C. (1987) · Journal of Clinical Investigation · doi:10.1172/jci113088 · Cited by 782
Metabolic Characteristics of Fibre Types in Human Skeletal Muscle
Essén, B., Jansson, E., Henriksson, J., Taylor, A. W., & Saltin, B. (1975) · Acta Physiologica Scandinavica · doi:10.1111/j.1748-1716.1975.tb10038.x · Cited by 582
Intramuscular lipid content is increased in obesity and decreased by weight loss
Goodpaster, B. H., Theriault, R., Watkins, S. C., & Kelley, D. E. (2000) · Metabolism · doi:10.1016/s0026-0495(00)80010-4 · Cited by 526
Self-citationSuction applied to a muscle biopsy maximizes sample size
Evans, W. J., Phinney, S. D., & Young, V. R. (1982) · Medicine & Science in Sports & Exercise · doi:10.1249/00005768-198201000-00018 · Cited by 525
Simoneau, J., Veerkamp, J. H., Turcotte, L. P., & Kelley, D. E. (1999) · The FASEB Journal · doi:10.1096/fasebj.13.14.2051 · Cited by 491
Self-citationHuman variation in skeletal muscle fiber-type proportion and enzyme activities
Simoneau, J. A., & Bouchard, C. (1989) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1989.257.4.e567 · Cited by 484
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Research works printed but not linked: 1
- Simoneau JA, Kelley DE . Skeletal muscle and obesity. In: Bray GA, Bouchard C, James WPT (eds). Handbook of obesity Marcel Dekker: New York 1998 539–553.
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The role of mitochondria in the pathogenesis of type 2 diabetes
Patti, M.-E., & Corvera, S. (2010) · Endocrine Reviews · doi:10.1210/er.2009-0027 · Cited by 551
Skeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance
Kiens, B. (2006) · Physiological Reviews · doi:10.1152/physrev.00023.2004 · Cited by 478
A second look into fibre typing – Relation to meat quality
Lefaucheur, L. (2009) · Meat Science · doi:10.1016/j.meatsci.2009.05.004 · Cited by 431
Lee, S., Bacha, F., Hannon, T., Kuk, J. L., Boesch, C., & Arslanian, S. (2012) · Diabetes · doi:10.2337/db12-0214 · Cited by 398
Hulver, M. W., Berggren, J. R., Carper, M. J., Miyazaki, M., Ntambi, J. M., Hoffman, E. P., Thyfault, J. P., Stevens, R., Dohm, G. L., Houmard, J. A., et al. (2005) · Cell Metabolism · doi:10.1016/j.cmet.2005.09.002 · Cited by 384
Metadata
- Authors
- Malenfant, P., Joanisse, D., Thériault, R., Goodpaster, B., Kelley, D., & Simoneau, J.-A.
- Affiliations
- Université Laval (CA)
- University of Pittsburgh (US)
- Corresponding author
- Kelley, DE
- ORCID
- Thériault, R
- Venue
- International Journal of Obesity
- Published
- 2001-09
- Volume
- 25
- Issue
- 9
- Pages
- 1316-1321
- ISSN
- 0307-0565 (ISSN-L)
- 1476-5497
- OpenAlex
- W2089177804
- Cited by
- 227
- References
- 31
- Citations by year
- 2026: 1 · 2025: 8 · 2024: 8 · 2023: 4 · 2022: 9 · 2021: 8 · 2020: 11 · 2019: 9 · 2018: 8 · 2017: 9 · 2016: 8 · 2015: 10 · 2014: 10 · 2013: 8 · 2012: 16
- FWCI
- 2.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- International Journal of Obesity (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK049200, R01 DK49200-03)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Keywords
- obesity, skeletal muscle, intracellular triglycerides, lipid droplets, muscle fiber type, vastus lateralis, lipid metabolism, fat deposition, myosin ATPase histochemistry, Oil Red O staining
- MeSH terms
- Adult, Azo Compounds, Biopsy, Needle, Coloring Agents, Female, Histocytochemistry, Humans, Male, Myosins, Obesity, Triglycerides, Muscle, Skeletal, Muscle Fibers, Skeletal
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