CoChat
Sign Up
Cited by 121Barrier Structure and Function StudiesHIV Research and TreatmentCell Adhesion Molecules Research

The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.

Signaling Mechanisms of HIV-1 Tat-Induced Alterations of Claudin-5 Expression in Brain Endothelial Cells

András, Ibolya E, Pu, Hong, Tian, Jing, Deli, Mária A, Nath, Avindra, Hennig, Bernhard, Toborek, Michal·2005·Journal of Cerebral Blood Flow & Metabolism
DOI 10.1038/sj.jcbfm.9600115
Open this paper in CoChat — free account

Abstract

Exposure of brain microvascular endothelial cells (BMEC) to human immunodeficiency virus-1 (HIV-1) Tat protein can decrease expression and change distribution of tight junction proteins, including claudin-5. Owing to the importance of claudin-5 in maintaining the blood–brain barrier (BBB) integrity, the present study focused on the regulatory mechanisms of Tat-induced alterations of claudin-5 mRNA and protein levels. Real-time reverse-transcription-polymerase chain reaction revealed that claudin-5 mRNA was markedly diminished in BMEC exposed to Tat. However, U0126 (an inhibitor of mitogen-activated protein kinase kinase1/2, MEK1/2) protected against this effect. In addition, inhibition of the vascular endothelial growth factor receptor type 2 (VEGFR-2) by SU1498, phosphatidylinositol-3 kinase (PI-3 K) by LY294002, nuclear factor-κB (NF-κB) by peptide SN50, and intracellular calcium by BAPTA/AM partially prevented Tat-mediated alterations in claudin-5 protein levels and immunoreactivity patterns. In contrast, inhibition of protein kinase C did not affect claudin-5 expression in Tat-treated cells. The present findings indicate that activation of VEGFR-2 and multiple redox-regulated signal transduction pathways are involved in Tat-induced alterations of claudin-5 expression. Because claudins constitute the major backbone of tight junctions, the present data are relevant to the disturbances of the BBB in the course of HIV-1 infection.

Overview

Integrity signals

Registry

Crossref · 10.1038/sj.jcbfm.9600115

Cites

59 of 59 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 5

  1. Barillari G, 1999, Blood, 94, 663
  2. Deli MA, 1997, Drug transport across the blood–brain barrier: in vivo and in vitro techniques, 23
  3. Rhodes RH, 1991, Pathol Annu, 26, 247
  4. Singer EJ, 1994, J Acquir Immune Defic Syndr, 7, 265
  5. Wu DT, 2000, J Neurovirol, 6, S82

Cited by

Sorted by citations

See all 121

Related

See all

Metadata

Authors
András, I. E., Pu, H., Tian, J., Deli, M. A., Nath, A., Hennig, B., & Toborek, M.
Affiliations
  • Department of Surgery, University of Kentucky, Lexington, Kentucky, USA
  • University of Kentucky (US)
  • HUN-REN Szegedi Biológiai Kutatóközpont (HU)
  • Johns Hopkins University (US)
ORCID
Venue
Journal of Cerebral Blood Flow & Metabolism
Publisher
SAGE Publications
Published
2005-09
Volume
25
Issue
9
Pages
1159-1170
ISSN
  • 0271-678X (ISSN-L)
  • 1559-7016
DOI
10.1038/sj.jcbfm.9600115
OpenAlex
W1973010584
Cited by
121
References
59
Citations by year
2025: 3 · 2024: 4 · 2023: 5 · 2022: 6 · 2021: 5 · 2020: 8 · 2019: 5 · 2018: 3 · 2017: 6 · 2016: 8 · 2015: 4 · 2014: 7 · 2013: 7 · 2012: 9
FWCI
4.03
Type
journal article
Language
en
Open access
closed
Licence
journals.sagepub.com/page/policies/text-and-data-mining-license (tdm)
Locations
  • Journal of Cerebral Blood Flow & Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r21aa013843-03)
  • National Institute on Alcohol Abuse and Alcoholism (AA 013843)
  • National Institute of Mental Health (MH 63022, R01MH063022)
  • National Institute of Neurological Disorders and Stroke (R01NS039254, NS-39254)
  • National Institute of Environmental Health Sciences (P42ES007380)
Topics
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Keywords
HIV-1 Tat protein, claudin-5, brain microvascular endothelial cells, blood-brain barrier, tight junction proteins, VEGFR2 signaling, PI3K signaling, NF-κB signaling, MEK1/2, redox signaling, intracellular calcium, protein kinase C, HIV-associated neurocognitive disorders
MeSH terms
Animals, Brain Chemistry, Calcium, Cells, Cultured, Chelating Agents, Membrane Proteins, Microscopy, Fluorescence, Genes, ras, RNA, Messenger, Blotting, Western, Signal Transduction, HIV-1, Down-Regulation, Gene Products, tat, NF-kappa B, Rats, Wistar, Reverse Transcriptase Polymerase Chain Reaction, Vascular Endothelial Growth Factor Receptor-2, Endothelial Cells, Rats, tat Gene Products, Human Immunodeficiency Virus, Claudin-5

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review