The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production
Open accessAbstract
Genetically engineered Escherichia coli KO11 is capable of efficiently producing ethanol from all sugar constituents of lignocellulose but lacks the high ethanol tolerance of yeasts currently used for commercial starch-based ethanol processes. Using an enrichment method which selects alternatively for ethanol tolerance during growth in broth and for ethanol production on solid medium, mutants of KO11 with increased ethanol tolerance were isolated which can produce more than 60 g ethanol L-1 from xylose in 72 h. Ethanol concentrations and yields achieved by the LY01 mutant with xylose exceed those reported for recombinant strains of Saccharomyces and Zymomonas mobilis, both of which have a high native ethanol tolerance.
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Cites
Mechanisms of membrane toxicity of hydrocarbons
Sikkema, J., de Bont, J. A., & Poolman, B. (1995) · Microbiological Reviews · doi:10.1128/mr.59.2.201-222.1995 · Cited by 2,685 · cited 2×
“Single step attempts failed to obtain clones capable of growth in 50 g ethanol L -1 . Previous studies have concluded that ethanol damages the permeability barrier provided by the cell membrane [9,12,34, 35 ].”
Background
Cited by
Adaptive laboratory evolution — harnessing the power of biology for metabolic engineering
Portnoy, V. A., Bezdan, D., & Zengler, K. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.03.007 · Cited by 309
Impact of ‘ome’ analyses on inverse metabolic engineering
Bro, C., & Nielsen, J. (2004) · Metabolic Engineering · doi:10.1016/j.ymben.2003.11.005 · Cited by 116
Metadata
- Authors
- Yomano, L. P., York, S. W., & Ingram, L. O.
- Venue
- Journal of Industrial Microbiology and Biotechnology
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-02-01
- Volume
- 20
- Issue
- 2
- Pages
- 132-138
- ISSN
- 1367-5435 (ISSN-L)
- 1476-5535
- OpenAlex
- W2082140226
- Cited by
- 258
- References
- 1
- FWCI
- 5.07
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production. https://app.cochat.ai/papers/10.1038/sj.jim.2900496
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- Yomano, L. P., York, S. W., & Ingram, L. O. (1998). Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production. Journal of Industrial Microbiology and Biotechnology. https://doi.org/10.1038/sj.jim.2900496
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
