The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Transgenic Enrichment of Cardiomyocytes From Human Embryonic Stem Cells
Open accessAbstract
To realize the full scientific and clinical potential of human embryonic stem cell (hESC)-cardiomyocytes, strategies to overcome the high degree of heterogeneity of differentiated populations are required. Here we demonstrate the utility of two transgenic approaches in enrichment of cardiomyocytes derived from HUES-7 cells: (i) negative selection of proliferating cells with the herpes simplex virus thymidine kinase/ganciclovir (HSVtk/GCV) suicide gene system; and (ii) positive selection of cardiomyocytes expressing a bicistronic reporter [green fluorescent protein (GFP)-internal ribosome entry site (IRES)-puromycin-N-acetyltransferase (PAC)] from the human alphamyosin heavy chain promoter. Parental and transgenic HUES-7 cells were similar with regard to morphology, pluripotency marker expression, differentiation, and cardiomyocyte electrophysiology. Whereas immunostaining of dissociated cardiomyocyte preparations expressing HSVtk or PAC contained <7% cardiomyocytes, parallel cultures treated with GCV or puromycin, respectively, contained 33.4 +/- 2.1% or 91.5 +/- 4.3% cardiomyocytes corresponding to an enrichment factor of 6.7- or 14.5-fold. Drug-selected cardiomyocytes responded to chronotropic stimulation and displayed cardiac-specific action potentials, demonstrating that functionality was retained. Both transgenic strategies will be generically applicable and should readily translate to the enrichment of many other differentiated lineages derived from hESCs.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture
Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M., & Smith, A. (2003) · Nature Biotechnology · doi:10.1038/nbt780 · Cited by 1,504
He, J.-Q., Ma, Y., Lee, Y., Thomson, J. A., & Kamp, T. J. (2003) · Circulation Research · doi:10.1161/01.res.0000080317.92718.99 · Cited by 800
The human embryonic stem cell-derived cardiomyocyte as a pharmacological model
Harding, S. E., Ali, N. N., Brito-Martins, M., & Gorelik, J. (2007) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2006.08.008 · Cited by 84
Cited by
Burridge, P. W., Keller, G., Gold, J. D., & Wu, J. C. (2012) · Cell Stem Cell · doi:10.1016/j.stem.2011.12.013 · Cited by 670
Nongenetic method for purifying stem cell–derived cardiomyocytes
Hattori, F., Chen, H., Yamashita, H., Tohyama, S., Satoh, Y.-S., Yuasa, S., Li, W., Yamakawa, H., Tanaka, T., Onitsuka, T., et al. (2010) · Nature Methods · doi:10.1038/nmeth.1403 · Cited by 416
Steps Toward Safe Cell Therapy Using Induced Pluripotent Stem Cells
Okano, H., Nakamura, M., Yoshida, K., Okada, Y., Tsuji, O., Nori, S., Ikeda, E., Yamanaka, S., & Miura, K. (2013) · Circulation Research · doi:10.1161/circresaha.111.256149 · Cited by 414
Matsa, E., Rajamohan, D., Dick, E., Young, L., Mellor, I., Staniforth, A., & Denning, C. (2011) · European Heart Journal · doi:10.1093/eurheartj/ehr073 · Cited by 389
Self-citationPrediction of drug-induced cardiotoxicity using human embryonic stem cell-derived cardiomyocytes
Braam, S. R., Tertoolen, L., van de Stolpe, A., Meyer, T., Passier, R., & Mummery, C. L. (2010) · Stem Cell Research · doi:10.1016/j.scr.2009.11.004 · Cited by 368
Metadata
- Authors
- Anderson, D., Self, T., Mellor, I. R., Goh, G., Hill, S. J., & Denning, C.
- Venue
- Molecular Therapy
- Publisher
- Elsevier BV
- Published
- 2007-11
- Volume
- 15
- Issue
- 11
- Pages
- 2027-2036
- ISSN
- 1525-0016 (ISSN-L)
- OpenAlex
- W2010177021
- Cited by
- 231
- References
- 3
- FWCI
- 7.90
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Transgenic Enrichment of Cardiomyocytes From Human Embryonic Stem Cells. https://app.cochat.ai/papers/10.1038/sj.mt.6300303
- The paper
- Anderson, D., Self, T., Mellor, I. R., Goh, G., Hill, S. J., & Denning, C. (2007). Transgenic Enrichment of Cardiomyocytes From Human Embryonic Stem Cells. Molecular Therapy. https://doi.org/10.1038/sj.mt.6300303
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
