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Cited by 68Stress Responses and CortisolNeurotransmitter Receptor Influence on BehaviorNeuroscience and Neuropharmacology Research

Stress-Induced c-Fos Expression is Differentially Modulated by Dexamethasone, Diazepam and Imipramine

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de Medeiros, Magda Alves, Carlos Reis, Luis, Eugênio Mello, Luiz·2005·Neuropsychopharmacology
DOI 10.1038/sj.npp.1300694
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Abstract

Immobilization stress upregulates c-Fos expression in several CNS areas. Repeated stress or the use of drugs can modulate stress-induced c-Fos expression. Here, we investigated in 40 different areas of the rat brain the effects of dexamethasone (SDX, a synthetic glucocorticoid), diazepam (SBDZ, a benzodiazepine), and imipramine (IMI, an antidepressant) on the c-Fos expression induced by restraint stress. Wistar rats were divided into four groups and submitted to 20 days of daily injection of saline (three first groups) or imipramine, 15 mg/kg, i.p. On day 21, animals were submitted to injections of saline (somatosensory, SS), SDX (1 mg/kg, i.p.), SBDZ (5 mg/kg, i.p.), or IMI (15 mg/kg, i.p.) before being submitted to restraint. Immediately after stress, the animals were perfused and their brains processed with immunohistochemistry for c-Fos (Ab-5 Oncogene Science). Dexamethasone reduced stress-induced c-Fos expression in SS cortex, hippocampus, paraventricular nucleus of the hypothalamus (PVH), and locus coeruleus (LC), whereas diazepam reduced c-Fos staining in the SS cortex, hippocampus, bed nucleus of stria terminalis, septal area, and hypothalamus (preoptic area and supramammillary nucleus). Chronic administration of imipramine decreased staining in the hippocampus, PVH, and LC, while increasing it in the nucleus raphe pallidus. We conclude that dexamethasone, diazepam and imipramine differentially modulate stress-induced Fos expression. The present study provides an important comparative background that may help in the further understanding of the effects of these compounds and on the brain activation as well as on the behavioral, neuroendocrine, and autonomic responses to stress.

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  • Several brain regions that innervate bone marrow have been linked to stress responses, reward processing and the pathogenesis of depression and anxiety disorders.

    “Notably, several of these bone marrow-innervating brain regions have been implicated in stress responses, reward processing and the pathogenesis of depression and anxiety disorders.”

    From Central regulation of stress-evoked peripheral immune responses (2023) · cites this paper as [48]

    The Introduction links several brain regions to stress responses, and “Modulation of inputs to PVH” links stress circuitry to depressive symptoms and identifies the lateral septum as a possible mediator of anxiety states (page numbers unavailable). Our copy does not establish that these regions innervate bone marrow or discuss their role in reward processing. (LLM, conf=0.86)

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  • Chronic stress activates hypothalamic regions including PVH, LH and ARH in rodents.

    “Similarly, regions of the hypothalamus, including the PVH, LH and ARH, are activated by chronic stress in rodents47–51”

    From Central regulation of stress-evoked peripheral immune responses (2023) · cites this paper as [48]

    The Results section (page unavailable) reports restraint-induced c-Fos expression in the rat hypothalamus, particularly the PVH. The Experimental Design section (page unavailable) shows that the rats underwent one hour of restraint, not chronic stress; the 20-day regimen was drug or saline treatment. The cited passages do not establish stress-induced activation of LH or ARH. (LLM, conf=0.88)

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  1. [12]12. Heninger GR, Charney DS (1987). Mechanism of action of antidepressant treatments: implications for the etiology and treatment of depressive disorders. In: Meltzer H (ed). Psycho- pharmacology. Raven Press: New York. pp 535-544.

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Authors
de Medeiros, M. A., Carlos Reis, L., & Eugênio Mello, L.
Venue
Neuropsychopharmacology
Publisher
Springer Science and Business Media LLC
Published
2005-02-16
Volume
30
Issue
7
Pages
1246-1256
ISSN
  • 0893-133X (ISSN-L)
  • 1740-634X
DOI
10.1038/sj.npp.1300694
OpenAlex
W1968549813
Cited by
68
References
38
Citations by year
2026: 1 · 2025: 5 · 2024: 5 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 6 · 2019: 2 · 2018: 1 · 2017: 7 · 2016: 3 · 2015: 4 · 2013: 3 · 2012: 3
FWCI
1.95
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Neuropsychopharmacology (publishedVersion)
  • PubMed (publishedVersion)
  • Americanae (AECID Library) (submittedVersion, other-oa)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
Funders
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (99/06114-9)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
Topics
  • Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
Fos expression, restraint stress, dexamethasone, diazepam, imipramine, glucocorticoids, benzodiazepines, antidepressants, hippocampus, paraventricular nucleus, locus coeruleus, bed nucleus of the stria terminalis, nucleus raphe pallidus, somatosensory cortex, hypothalamus, preoptic area, supramammillary nucleus, septum
MeSH terms
Analysis of Variance, Animals, Antidepressive Agents, Tricyclic, Brain, Cell Count, Dexamethasone, Diazepam, Gene Expression Regulation, Glucocorticoids, Imipramine, Immunohistochemistry, Male, Restraint, Physical, Stress, Psychological, Anti-Anxiety Agents, Proto-Oncogene Proteins c-fos, Rats, Wistar, Rats

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