DUSP22/LMW-DSP2 regulates estrogen receptor-α-mediated signaling through dephosphorylation of Ser-118
Open accessAbstract
In the previous study, we demonstrated the involvement of dual specificity phosphatase 22 (DUSP22/LMW-DSP2) in regulating the leukemia inhibitory factor/interleukin-6/signal transducer and activator of transcription 3-mediated signaling pathway. In this study, we show beta-estradiol (E2)-induced DUSP22 mRNA expression in estrogen receptor alpha (ERalpha)-positive breast cancer cells, whereas E2-induced phosphorylation and activation of ERalpha was suppressed by overexpression of DUSP22 but not catalytically inactive mutants. Furthermore, small-interfering RNA-mediated reduction of DUSP22 expression enhanced ERalpha-mediated transcription and endogenous gene expression. In fact, DUSP22 associated with ERalpha in vivo and both endogenous proteins interacted in ERalpha-positive breast cancer T47D cells. These results strongly suggest that DUSP22 acts as a negative regulator of the ERalpha-mediated signaling pathway.
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From Altered genome-wide methylation in endometriosis (2014) · cites this paper as [33]
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Dual-specificity phosphatases: critical regulators with diverse cellular targets
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- Authors
- Sekine, Y., Ikeda, O., Hayakawa, Y., Tsuji, S., Imoto, S., Aoki, N., Sugiyama, K., & Matsuda, T.
- Affiliations
- Hokkaido University (JP)
- Mie University (JP)
- Boehringer Ingelheim (Japan) (JP)
- Corresponding author
- Matsuda, T
- Venue
- Oncogene
- Published
- 2007-03-26
- Volume
- 26
- Issue
- 41
- Pages
- 6038-6049
- ISSN
- 0950-9232 (ISSN-L)
- 1476-5594
- OpenAlex
- W2019924027
- Cited by
- 74
- References
- 43
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 2 · 2022: 5 · 2021: 9 · 2019: 4 · 2018: 2 · 2017: 5 · 2016: 4 · 2015: 4 · 2014: 4 · 2013: 6 · 2012: 1
- FWCI
- 3.75
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Oncogene (publishedVersion)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion)
- Hokkaido University Collection of Scholarly and Academic Papers (Hokkaido University) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Breast Cancer Treatment Studies
- Keywords
- DUSP22, estrogen receptor alpha, beta-estradiol, ERα phosphorylation, ERα-positive breast cancer, negative transcriptional regulation, protein-protein interaction, T47D cells
- MeSH terms
- Breast Neoplasms, Estradiol, Female, HeLa Cells, Humans, Phosphoprotein Phosphatases, Phosphoserine, RNA, Messenger, Tumor Cells, Cultured, Signal Transduction, Transcriptional Activation, Polymerase Chain Reaction, Protein Tyrosine Phosphatases, Estrogen Receptor alpha, Dual-Specificity Phosphatases, Mitogen-Activated Protein Kinase Phosphatases
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