The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
DNA Nanostructure-based Interfacial engineering for PCR-free ultrasensitive electrochemical analysis of microRNA
Open accessAbstract
MicroRNAs (miRNAs) have been identified as promising cancer biomarkers due to their stable presence in serum. As an alternative to PCR-based homogenous assays, surface-based electrochemical biosensors offer great opportunities for low-cost, point-of-care tests (POCTs) of disease-associated miRNAs. Nevertheless, the sensitivity of miRNA sensors is often limited by mass transport and crowding effects at the water-electrode interface. To address such challenges, we herein report a DNA nanostructure-based interfacial engineering approach to enhance binding recognition at the gold electrode surface and drastically improve the detection sensitivity. By employing this novel strategy, we can directly detect as few as attomolar (<1, 000 copies) miRNAs with high single-base discrimination ability. Given that this ultrasensitive electrochemical miRNA sensor (EMRS) is highly reproducible and essentially free of prior target labeling and PCR amplification, we also demonstrate its application by analyzing miRNA expression levels in clinical samples from esophageal squamous cell carcinoma (ESCC) patients.
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Cites
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
Lee, R. C., Feinbaum, R. L., & Ambros, V. (1993) · Cell · doi:10.1016/0092-8674(93)90529-y · Cited by 12,948
Cited by
Integrated Magneto–Electrochemical Sensor for Exosome Analysis
Jeong, S., Park, J., Pathania, D., Castro, C. M., Weissleder, R., & Lee, H. (2016) · ACS Nano · doi:10.1021/acsnano.5b07584 · Cited by 469
Functional DNA Nanostructures for Theranostic Applications
Pei, H., Zuo, X., Zhu, D., Huang, Q., & Fan, C. (2014) · Accounts of Chemical Research · doi:10.1021/ar400195t · Cited by 409
Self-citationKelley, S. O., Mirkin, C. A., Walt, D. R., Ismagilov, R. F., Toner, M., & Sargent, E. H. (2014) · Nature Nanotechnology · doi:10.1038/nnano.2014.261 · Cited by 398
Azimzadeh, M., Rahaie, M., Nasirizadeh, N., Ashtari, K., & Naderi-Manesh, H. (2016) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2015.09.020 · Cited by 342
Kilic, T., Erdem, A., Ozsoz, M., & Carrara, S. (2018) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2017.08.007 · Cited by 299
Metadata
- Authors
- Wen, Y., Pei, H., Shen, Y., Xi, J., Lin, M., Lu, N., Shen, X., Li, J., & Fan, C.
- Venue
- Scientific Reports
- Published
- 2012-11-16
- Volume
- 2
- Issue
- 1
- Pages
- 867-867
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2164421479
- Cited by
- 222
- References
- 1
- FWCI
- 6.85
- Type
- journal article
- Language
- en
- Open access
- gold
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- Wen, Y., Pei, H., Shen, Y., Xi, J., Lin, M., Lu, N., Shen, X., Li, J., & Fan, C. (2012). DNA Nanostructure-based Interfacial engineering for PCR-free ultrasensitive electrochemical analysis of microRNA. Scientific Reports. https://doi.org/10.1038/srep00867
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
