The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity
Open accessAbstract
Transcription activator-like effector (TALE) nuclease (TALEN) is a site-specific nuclease, which can be freely designed and easily constructed. Numerous methods of constructing TALENs harboring different TALE scaffolds and repeat variants have recently been reported. However, the functionalities of structurally different TALENs have not yet been compared. Here, we report on the functional differences among several types of TALENs targeting the same loci. Using HEK293T cell-based single-strand annealing and Cel-I nuclease assays, we found that TALENs with periodically-patterned repeat variants harboring non-repeat-variable di-residue (non-RVD) variations (Platinum TALENs) showed higher activities than TALENs without non-RVD variations. Furthermore, the efficiencies of gene disruption mediated by Platinum TALENs in frogs and rats were significantly higher than in previous reports. This study therefore demonstrated an efficient system for the construction of these highly active Platinum TALENs (Platinum Gate system), which could establish a new standard in TALEN engineering.
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Cites
A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity
Mussolino, C., Morbitzer, R., Lütge, F., Dannemann, N., Lahaye, T., & Cathomen, T. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr597 · Cited by 714
Cited by
Li, H. L., Fujimoto, N., Sasakawa, N., Shirai, S., Ohkame, T., Sakuma, T., Tanaka, M., Amano, N., Watanabe, A., Sakurai, H., et al. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.10.013 · Cited by 471
Self-citationMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system
Sakuma, T., Nishikawa, A., Kume, S., Chayama, K., & Yamamoto, T. (2014) · Scientific Reports · doi:10.1038/srep05400 · Cited by 352
Self-citationPlant organellar genomes: much done, much more to do
Wang, J., Kan, S., Liao, X., Zhou, J., Tembrock, L. R., Daniell, H., Jin, S., & Wu, Z. (2024) · Trends in Plant Science · doi:10.1016/j.tplants.2023.12.014 · Cited by 280
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
Kaneko, T., Sakuma, T., Yamamoto, T., & Mashimo, T. (2014) · Scientific Reports · doi:10.1038/srep06382 · Cited by 204
Self-citationSato, K., Oiwa, R., Kumita, W., Henry, R., Sakuma, T., Ito, R., Nozu, R., Inoue, T., Katano, I., Sato, K., et al. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.06.003 · Cited by 159
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Metadata
- Authors
- Sakuma, T., Ochiai, H., Kaneko, T., Mashimo, T., Tokumasu, D., Sakane, Y., Suzuki, K.-I., Miyamoto, T., Sakamoto, N., Matsuura, S., & Yamamoto, T.
- Venue
- Scientific Reports
- Published
- 2013-11-29
- Volume
- 3
- Issue
- 1
- Pages
- 3379-3379
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2053745385
- Cited by
- 235
- References
- 1
- FWCI
- 11.70
- Type
- journal article
- Language
- en
- Open access
- gold
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- Sakuma, T., Ochiai, H., Kaneko, T., Mashimo, T., Tokumasu, D., Sakane, Y., Suzuki, K.-I., Miyamoto, T., Sakamoto, N., Matsuura, S., & Yamamoto, T. (2013). Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Scientific Reports. https://doi.org/10.1038/srep03379
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