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Cited by 20RNA Research and SplicingRNA regulation and diseaseFungal and yeast genetics research

The exosome controls alternative splicing by mediating the gene expression and assembly of the spliceosome complex

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Zhang, Lin, Wan, Yufeng, Huang, Guobin, Wang, Dongni, Yu, Xinyang, Huang, Guocun, Guo, Jinhu·2015·Scientific Reports
DOI 10.1038/srep13403PMC PMC4549623
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Abstract

The exosome is a complex with exoribonuclease activity that regulates RNA surveillance and turnover. The exosome also plays a role in regulating the degradation of precursor mRNAs to maintain the expression of splicing variants. In Neurospora, the silencing of rrp44, which encodes the catalytic subunit of the exosome, changed the expression of a set of spliceosomal snRNA, snRNP genes and SR protein related genes. The knockdown of rrp44 also affected the assembly of the spliceosome. RNA-seq analysis revealed a global change in bulk splicing events. Exosome-mediated splicing may regulate alternative splicing of NCU05290, NCU07421 and the circadian clock gene frequency(frq). The knockdown of rrp44 led to an increased ratio of splicing variants without intron 6 (I-6) and shorter protein isoform small FRQ (s-FRQ) as a consequence. These findings suggest that the exosome controls splicing events by regulating the degradation of precursor mRNAs and the gene expression, assembly and function of the spliceosome.

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  • The RNA exosome has specialized functions in RNA splicing and regulation of AU-rich element (ARE)-containing transcripts.

    “In addition to RNA turnover, the RNA exosome has specialized functions in RNA splicing and the regulation of AU-rich element (ARE)-containing transcripts44–47.”

    From EXOSC3 G191 Variants Trigger System-Wide Recalibration of RNA Processing Machinery (2026) · cites this paper as [45]

    The Abstract and Discussion report that the exosome regulates splicing, including through effects on spliceosome components. The passages from our copy do not address regulation of AU-rich element (ARE)-containing transcripts; searches for that term and AU-rich mRNA regulation found no supporting passage. Page numbers were not provided. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • The RNA exosome complex ensures precise intron removal and spliceosomal RNA processing, critical steps in maturation of protein-coding transcripts.

    “The RNA exosome complex also ensures precise intron removal and spliceosomal RNA processing, critical steps in the maturation of protein-coding transcripts45.”

    From EXOSC3 G191 Variants Trigger System-Wide Recalibration of RNA Processing Machinery (2026) · cites this paper as [45]

    The Discussion (page not provided) says the exosome regulates spliceosome assembly and the processing and turnover of snRNAs. The Results report altered intron retention and splice-site selection after rrp44 knockdown in Neurospora. These findings support a role in splicing, but the claim that the exosome broadly ensures precise intron removal during protein-coding transcript maturation is stronger than the paper establishes. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

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Cited by

Sorted by citations

Authors
Zhang, L., Wan, Y., Huang, G., Wang, D., Yu, X., Huang, G., & Guo, J.
Affiliations
Sun Yat-sen University (CN)
Corresponding author
Guo, Jinhu
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2015-08-26
Volume
5
Issue
1
Pages
13403-13403
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/srep13403
PMC
PMC4549623
OpenAlex
W2414696619
Cited by
20
References
1
Citations by year
2026: 1 · 2023: 3 · 2022: 2 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 6 · 2017: 2 · 2015: 1
FWCI
1.30
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (973 Program, 2011CB711000, 31071122, 31171119)
  • National Key Research and Development Program of China (2012CB947600, 2011CB711000)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
exosomes, alternative splicing, spliceosome assembly, Neurospora crassa, RNA surveillance, splice variants, circadian clock, small nuclear RNA, small nuclear ribonucleoproteins, SR proteins, gene expression regulation
MeSH terms
Gene Expression Regulation, Neurospora, RNA Precursors, Spliceosomes, Alternative Splicing, RNA Splice Sites, MicroRNAs, Exosomes

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