The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Clinical utility of circulating tumor DNA for molecular assessment in pancreatic cancer
Open accessAbstract
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. The genomic landscape of the PDAC genome features four frequently mutated genes (KRAS, CDKN2A, TP53 and SMAD4) and dozens of candidate driver genes altered at low frequency, including potential clinical targets. Circulating cell-free DNA (cfDNA) is a promising resource to detect and monitor molecular characteristics of tumors. In the present study, we determined the mutational status of KRAS in plasma cfDNA using multiplex picoliter-droplet digital PCR in 259 patients with PDAC. We constructed a novel modified SureSelect-KAPA-Illumina platform and an original panel of 60 genes. We then performed targeted deep sequencing of cfDNA and matched germline DNA samples in 48 patients who had ≥1% mutant allele frequencies of KRAS in plasma cfDNA. Importantly, potentially targetable somatic mutations were identified in 14 of 48 patients (29.2%) examined by targeted deep sequencing of cfDNA. We also analyzed somatic copy number alterations based on the targeted sequencing data using our in-house algorithm and potentially targetable amplifications were detected. Assessment of mutations and copy number alterations in plasma cfDNA may provide a prognostic and diagnostic tool to assist decisions regarding optimal therapeutic strategies for PDAC patients.
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Whole genomes redefine the mutational landscape of pancreatic cancer
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“Sixty genes were selected including recurrent mutated genes previously identified in PDAC 2–4 and drug-targetable genes whose alterations were considered most likely to be clinically relevant (Supplementary Table S5).”
Distant metastasis occurs late during the genetic evolution of pancreatic cancer
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Self-citationPancreatic cancer genomes reveal aberrations in axon guidance pathway genes
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Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA
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The patterns and dynamics of genomic instability in metastatic pancreatic cancer
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Self-citationMapping copy number variation by population-scale genome sequencing
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“2) 21,22 .”
InfluentialTrans-ancestry mutational landscape of hepatocellular carcinoma genomes
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Cited by
Integrating liquid biopsies into the management of cancer
Siravegna, G., Marsoni, S., Siena, S., & Bardelli, A. (2017) · Nature Reviews Clinical Oncology · doi:10.1038/nrclinonc.2017.14 · Cited by 1,908
Role of oncogenic KRAS in the diagnosis, prognosis and treatment of pancreatic cancer
Buscail, L., Bournet, B., & Cordelier, P. (2020) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-019-0245-4 · Cited by 721
Gorgannezhad, L., Umer, M., Islam, M. N., Nguyen, N.-T., & Shiddiky, M. J. A. (2018) · Lab on a Chip · doi:10.1039/c8lc00100f · Cited by 312
Metadata
- Authors
- Takai, E., Totoki, Y., Nakamura, H., Morizane, C., Nara, S., Hama, N., Suzuki, M., Furukawa, E., Kato, M., Hayashi, H., Kohno, T., Ueno, H., Shimada, K., Okusaka, T., Nakagama, H., Shibata, T., & Yachida, S.
- Venue
- Scientific Reports
- Published
- 2015-12-16
- Volume
- 5
- Issue
- 1
- Pages
- 18425-18425
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2208134400
- Cited by
- 192
- References
- 14
- FWCI
- 5.99
- Type
- journal article
- Language
- en
- Open access
- gold
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- Takai, E., Totoki, Y., Nakamura, H., Morizane, C., Nara, S., Hama, N., Suzuki, M., Furukawa, E., Kato, M., Hayashi, H., Kohno, T., Ueno, H., Shimada, K., Okusaka, T., Nakagama, H., Shibata, T., & Yachida, S. (2015). Clinical utility of circulating tumor DNA for molecular assessment in pancreatic cancer. Scientific Reports. https://doi.org/10.1038/srep18425
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
