The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Efficient Genome Editing in Apple Using a CRISPR/Cas9 system
Open accessAbstract
Genome editing is a powerful technique for genome modification in molecular research and crop breeding and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the CRISPR/Cas9 system. Four guide RNAs (gRNAs) were designed and stably transformed with Cas9 separately in apple. Clear and partial albino phenotypes were observed in 31.8% of regenerated plantlets for one gRNA and bi-allelic mutations in apple PDS were confirmed by DNA sequencing. In addition, an 18-bp gRNA also induced a targeted mutation. These CRIPSR/Cas9 induced-mutations in the apple genome suggest activation of the NHEJ pathway, but with some involvement also of the HR pathway. Our results demonstrate that genome editing can be practically applied to modify the apple genome.
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Cites
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
Fu, Y., Sander, J. D., Reyon, D., Cascio, V. M., & Joung, J. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2808 · Cited by 1,993
Cited by
DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins
Malnoy, M., Viola, R., Jung, M.-H., Koo, O.-J., Kim, S., Kim, J.-S., Velasco, R., & Nagamangala Kanchiswamy, C. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01904 · Cited by 756
Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants
Osakabe, Y., Watanabe, T., Sugano, S. S., Ueta, R., Ishihara, R., Shinozaki, K., & Osakabe, K. (2016) · Scientific Reports · doi:10.1038/srep26685 · Cited by 367
Self-citationCRISPR/Cas9‐mediated efficient targeted mutagenesis in grape in the first generation
Wang, X., Tu, M., Wang, D., Liu, J., Li, Y., Li, Z., Wang, Y., & Wang, X. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12832 · Cited by 367
Gene Editing and Crop Improvement Using CRISPR-Cas9 System
Arora, L., & Narula, A. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01932 · Cited by 367
Reduced Drought Tolerance by CRISPR/Cas9-Mediated SlMAPK3 Mutagenesis in Tomato Plants
Wang, L., Chen, L., Li, R., Zhao, R., Yang, M., Sheng, J., & Shen, L. (2017) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.7b02745 · Cited by 350
Metadata
- Authors
- Nishitani, C., Hirai, N., Komori, S., Wada, M., Okada, K., Osakabe, K., Yamamoto, T., & Osakabe, Y.
- Venue
- Scientific Reports
- Published
- 2016-08-17
- Volume
- 6
- Issue
- 1
- Pages
- 31481-31481
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2510901304
- Cited by
- 350
- References
- 1
- FWCI
- 13.69
- Type
- journal article
- Language
- en
- Open access
- gold
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- Nishitani, C., Hirai, N., Komori, S., Wada, M., Okada, K., Osakabe, K., Yamamoto, T., & Osakabe, Y. (2016). Efficient Genome Editing in Apple Using a CRISPR/Cas9 system. Scientific Reports. https://doi.org/10.1038/srep31481
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