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Cited by 367

The DOI matches its Crossref record; 135 of the 135 references it cites were found in a registry or an index.

Gene Editing and Crop Improvement Using CRISPR-Cas9 System

Open access
Arora, Leena, Narula, Alka·2017·Frontiers in Plant Science

Abstract

Advancements in Genome editing technologies have revolutionized the fields of functional genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat)-Cas9 is a multipurpose technology for genetic engineering that relies on the complementarity of the guideRNA (gRNA) to a specific sequence and the Cas9 endonuclease activity. It has broadened the agricultural research area, bringing in new opportunities to develop novel plant varieties with deletion of detrimental traits or addition of significant characters. This RNA guided genome editing technology is turning out to be a groundbreaking innovation in distinct branches of plant biology. CRISPR technology is constantly advancing including options for various genetic manipulations like generating knockouts; making precise modifications, multiplex genome engineering, and activation and repression of target genes. The review highlights the progression throughout the CRISPR legacy. We have studied the rapid evolution of CRISPR/Cas9 tools with myriad functionalities, capabilities, and specialized applications. Among varied diligences, plant nutritional improvement, enhancement of plant disease resistance and production of drought tolerant plants are reviewed. The review also includes some information on traditional delivery methods of Cas9-gRNA complexes into plant cells and incorporates the advent of CRISPR ribonucleoproteins (RNPs) that came up as a solution to various limitations that prevailed with plasmid-based CRISPR system.

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Metadata

Authors
Arora, L., & Narula, A.
Venue
Frontiers in Plant Science
Published
2017-11-08
Volume
8
Pages
1932-1932
ISSN
1664-462X (ISSN-L)
OpenAlex
W2768052018
Cited by
367
References
135
FWCI
9.61
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: Gene Editing and Crop Improvement Using CRISPR-Cas9 System. https://app.cochat.ai/papers/10.3389/fpls.2017.01932
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Arora, L., & Narula, A. (2017). Gene Editing and Crop Improvement Using CRISPR-Cas9 System. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2017.01932

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