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Cited by 109Multiple Sclerosis Research StudiesMesenchymal stem cell researchExtracellular vesicles in disease

The secretome of periodontal ligament stem cells from MS patients protects against EAE

Open access
Rajan, Thangavelu Soundara, Giacoppo, Sabrina, Diomede, Francesca, Ballerini, Patrizia, Paolantonio, Michele, Marchisio, Marco, Piattelli, Adriano, Bramanti, Placido +2 more·2016·Scientific Reports
DOI 10.1038/srep38743PMC PMC5141419
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Abstract

Manipulation of stem cells or stem cells-derived secretome has emerged as a novel alternative therapeutic option for multiple sclerosis (MS). Here we show that human periodontal ligament stem cells (hPDLSCs)-derived conditioned medium (hPDLSCs-CM) and purified exosomes/microvesicles (hPDLSCs-EMVs) obtained from Relapsing Remitting (RR)-MS patients and healthy donors block experimental autoimmune encephalomyelitis (EAE), a mouse model of MS, by inducing anti-inflammatory and immunosuppressive effects in spinal cord and spleen, and reverse disease progression by restoring tissue integrity via remyelination in the spinal cord. We show that hPDLSCs-CM and hPDLSCs-EMVs reduce pro-inflammatory cytokines IL-17, IFN-γ, IL-1β, IL-6, TNF-α, and induce anti-inflammatory IL-10. In addition, apoptosis related STAT1, p53, Caspase 3, and Bax expressions were attenuated. Our findings unravel the immunosuppressive effects of hPDLSCs-CM and hPDLSCs-EMVs in EAE mice, and suggest simple alternative autologous source for patient-customized cell-free targeting treatment in MS patients.

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Cited by

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Authors
Rajan, T. S., Giacoppo, S., Diomede, F., Ballerini, P., Paolantonio, M., Marchisio, M., Piattelli, A., Bramanti, P., Mazzon, E., & Trubiani, O.
Affiliations
  • Centro Neurolesi Bonino Pulejo (IT)
  • University of Chieti-Pescara (IT)
Corresponding author
Mazzon, Emanuela
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2016-12-07
Volume
6
Issue
1
Pages
38743-38743
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/srep38743
PMC
PMC5141419
OpenAlex
W2560630866
Cited by
109
References
2
Citations by year
2026: 6 · 2025: 5 · 2024: 9 · 2023: 12 · 2022: 14 · 2021: 13 · 2020: 13 · 2019: 15 · 2018: 13 · 2017: 9
FWCI
11.21
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
multiple sclerosis, periodontal ligament stem cells, secretome, exosomes, microvesicles, conditioned medium, experimental autoimmune encephalomyelitis, immunosuppression, remyelination, inflammatory cytokines, anti-inflammatory cytokines, apoptosis, cell-free therapy, autologous stem cell therapy
MeSH terms
Animals, Encephalomyelitis, Autoimmune, Experimental, Female, Humans, Male, Multiple Sclerosis, Periodontal Ligament, Stem Cells, Cytokines, Apoptosis Regulatory Proteins, Mice, Cell-Derived Microparticles, Exosomes

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