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Cited by 50Spaceflight effects on biologyBone Metabolism and DiseasesBone health and osteoporosis research

Acute transcriptional up-regulation specific to osteoblasts/osteoclasts in medaka fish immediately after exposure to microgravity

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Chatani, Masahiro, Morimoto, Hiroya, Takeyama, Kazuhiro, Mantoku, Akiko, Tanigawa, Naoki, Kubota, Koji, Suzuki, Hiromi, Uchida, Satoko +7 more·2016·Scientific Reports
DOI 10.1038/srep39545PMC PMC5177882
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Abstract

Bone loss is a serious problem in spaceflight; however, the initial action of microgravity has not been identified. To examine this action, we performed live-imaging of animals during a space mission followed by transcriptome analysis using medaka transgenic lines expressing osteoblast and osteoclast-specific promoter-driven GFP and DsRed. In live-imaging for osteoblasts, the intensity of osterix- or osteocalcin-DsRed fluorescence in pharyngeal bones was significantly enhanced 1 day after launch; and this enhancement continued for 8 or 5 days. In osteoclasts, the signals of TRAP-GFP and MMP9-DsRed were highly increased at days 4 and 6 after launch in flight. HiSeq from pharyngeal bones of juvenile fish at day 2 after launch showed up-regulation of 2 osteoblast- and 3 osteoclast- related genes. Gene ontology analysis for the whole-body showed that transcription of genes in the category “nucleus” was significantly enhanced; particularly, transcription-regulators were more up-regulated at day 2 than at day 6. Lastly, we identified 5 genes, c-fos, jun-B-like, pai-1, ddit4 and tsc22d3, which were up-regulated commonly in the whole-body at days 2 and 6, and in the pharyngeal bone at day 2. Our results suggested that exposure to microgravity immediately induced dynamic alteration of gene expression levels in osteoblasts and osteoclasts.

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  • Conditions of microgravity and simulated microgravity decrease the mineral content and cortical and trabecular microstructures of bone.

    “conditions of microgravity and simulated microgravity decrease the mineral content and cortical and trabecular microstructures of bone”

    From Osteoclasts and Microgravity (2020) · cites this paper as [61]

    The Discussion (page unavailable) says bone mineral density decreases under microgravity, and the Abstract reports bone and calcium loss during spaceflight. The copy does not say that simulated microgravity decreases bone mineral content or that either condition degrades cortical and trabecular microstructures. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • Conditions of microgravity and simulated microgravity increase osteoclast secretion of sclerostin.

    “increase osteoclast secretion of sclerostin”

    From Osteoclasts and Microgravity (2020) · cites this paper as [61]

    The Discussion reports increased osteoclast signals and expression of osteoclast-related genes in medaka exposed to actual microgravity. It does not report osteoclast secretion of sclerostin or establish that simulated microgravity has that effect; the Discussion distinguishes this study from earlier work using artificial microgravity. No page numbers are available in our copy. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Conditions of microgravity and simulated microgravity increase osteoclast differentiation, fusion, and expression of regulatory and osteoclast-specific genes.

    “increase osteoclast differentiation, fusion, and expression of regulatory and osteoclast-specific genes”

    From Osteoclasts and Microgravity (2020) · cites this paper as [61]

    The Discussion and Results (page numbers unavailable) report increased osteoclast-related gene expression in medaka exposed to actual microgravity, along with up-regulation of transcription-regulatory genes. The paper does not show increased osteoclast differentiation or fusion after exposure, and its experiment did not test simulated microgravity. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • In medaka fish during spaceflight, in vivo signals of osteoclast activity were upregulated at days 4 and 6 after launch, and expression of osteoclast-specific genes TRAP, cathepsin K, and MMP9 were upregulated on day two of the flight.

    “They found that in vivo signals of osteoclast activity were upregulated at days 4 and 6 after launch, and that expression of osteoclast-specific genes TRAP, cathepsin K, and MMP9 were upregulated on day two of the flight [61].”

    From Osteoclasts and Microgravity (2020) · cites this paper as [61]

  • Conditions of microgravity and simulated microgravity decrease osteoblastogenesis and osteoblast secretion of OPG.

    “decrease osteoblastogenesis and osteoblast secretion of OPG”

    From Osteoclasts and Microgravity (2020) · cites this paper as [61]

    The Results section, “Enhancement of osteoblast signals under microgravity” (page unavailable), reports increased osteoblast-marker fluorescence in medaka, but does not measure osteoblastogenesis. The Discussion (page unavailable) describes altered osteoblast gene expression; neither passage addresses OPG secretion or establishes what simulated microgravity does to either outcome. Searches for OPG/osteoprotegerin and decreased osteoblastogenesis found no decisive passage. (LLM, conf=0.72)

    Experimental — accuracy not yet measured

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Cited by

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Authors
Chatani, M., Morimoto, H., Takeyama, K., Mantoku, A., Tanigawa, N., Kubota, K., Suzuki, H., Uchida, S., Tanigaki, F., Shirakawa, M., Gusev, O., Sychev, V., Takano, Y., Itoh, T., & Kudo, A.
Affiliations
  • Tokyo Institute of Technology (JP)
  • Chiyoda Corporation (Japan) (JP)
  • Japan Space Forum (JP)
  • Japan Aerospace Exploration Agency (JP)
  • Kazan Federal University (RU)
  • Institute of Biomedical Problems (RU)
  • Tokyo Medical and Dental University (JP)
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2016-12-22
Volume
6
Issue
1
Pages
39545-39545
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/srep39545
PMC
PMC5177882
OpenAlex
W2564130120
Cited by
50
References
2
Citations by year
2026: 4 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 8 · 2021: 9 · 2020: 6 · 2019: 6 · 2018: 9 · 2017: 1 · 2015: 1
FWCI
4.29
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Russian Science Foundation
  • Japan Society for the Promotion of Science (16K15778, 16H01635, 15H05935, 25514003)
  • Japan Aerospace Exploration Agency
Topics
  • Health Sciences › Medicine › Physiology › Spaceflight effects on biology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
Keywords
microgravity, spaceflight, osteoblasts, osteoclasts, bone loss, medaka, pharyngeal bones, transcriptional upregulation, Osterix, osteocalcin, traps, MMP-9, live imaging, transcription regulators, c-fos, plasminogen activator inhibitor-1, DDIT4, TSC22D3, gene expression
MeSH terms
Animals, Bone and Bones, Fishes, Gene Expression Regulation, Luminescent Proteins, Oryzias, Osteoblasts, Osteoclasts, Space Flight, Transcription, Genetic, Weightlessness, Osteocalcin, Up-Regulation, Gene Expression, Animals, Genetically Modified, Transcriptome

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