Cited by 208Insect Resistance and GeneticsDengue and Mosquito Control ResearchMalaria Research and Control

Detection of a new pyrethroid resistance mutation (V410L) in the sodium channel of Aedes aegypti: a potential challenge for mosquito control

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Haddi, Khalid, Tomé, Hudson V. V., Du, Yuzhe, Valbon, Wilson R., Nomura, Yoshiko, Martins, Gustavo F., Dong, Ke, Oliveira, Eugênio E.·2017·Scientific Reports

Abstract

The yellow fever mosquito,Aedes aegypti, particularly in Neotropical regions, is the principal vector of dengue, yellow fever, Zika and Chikungunya viruses. Pyrethroids remain one of the most used insecticides to control Aedes mosquitoes, despite the development of pyrethroid resistance in many mosquito populations worldwide. Here, we report a Brazilian strain of A. aegypti with high levels (approximately 100–60,000 fold) of resistance to both type I and type II pyrethroids. We detected two mutations (V410L and F1534C) in the sodium channel from this resistant strain. This study is the first report of the V410L mutation in mosquitoes. Alone or in combination with the F1534C mutation, the V410L mutation drastically reduced the sensitivity of mosquito sodium channels expressed in Xenopus oocytes to both type I and type II pyrethroids. The V410L mutation presents a serious challenge for the control of A. aegypti and will compromise the use of pyrethroids for the control of A. aegypti in Brazil; therefore, early monitoring of the frequency of the V410L mutation will be a key resistance management strategy to preserve the effectiveness of pyrethroid insecticides.

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  1. Araújo, A. P. Análise da resistência a inseticidas químicos em populações de Aedes aegypti (Diptera: Culicidae), de Municípios do Estado de Pernambuco PhD thesis, Fundação Oswaldo Cruz, Recife-PE, 120p (2013).
  2. Feyereisen R. In Comprehensive Molecular Insect Sciencevol. 4, Elsevier, Oxford, pp. 1– 77. (eds Gilbert L. I., Latrou K. & Gill S.) (2005).
  3. Ranson H. & Hemingway J. In Comprehensive Molecular Insect Science, vol. 5, Elsevier, Oxford, pp. 383– 402. (eds Gilbert L. I., Latrou K. & Gill S.) (2005).
  4. Warmke J. W.. Functional expression of Drosophila para sodium channels modulation by the membrane protein TipE and toxin pharmacology. The Journal of general physiology 110, 119– 133 (1997). 9236205 PMC2233785

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Authors
Haddi, K., Tomé, H. V. V., Du, Y., Valbon, W. R., Nomura, Y., Martins, G. F., Dong, K., & Oliveira, E. E.
Affiliations
  • Universidade Federal de Viçosa (BR)
  • Michigan State University (US)
Corresponding author
Haddi, Khalid
Venue
Scientific Reports
Published
2017-04-19
Volume
7
Issue
1
Pages
46549-46549
ISSN
2045-2322 (ISSN-L)
OpenAlex
W2606667865
Cited by
208
References
41
Citations by year
2026: 16 · 2025: 25 · 2024: 21 · 2023: 20 · 2022: 21 · 2021: 37 · 2020: 31 · 2019: 20 · 2018: 10 · 2017: 6
FWCI
7.49
Type
journal article
Language
en
Open access
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • Americanae (AECID Library) (submittedVersion, other-oa)
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Fundação de Amparo à Pesquisa do Estado de Minas Gerais
  • Fundação Arthur Bernardes
  • National Institutes of Health
  • Ciência sem Fronteiras
  • National Institute of General Medical Sciences (R01GM057440)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
  • Social Sciences › Sociology and Political Science › Dengue and Mosquito Control Research
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
Keywords
Aedes aegypti, pyrethroid resistance, F1534C mutation, voltage-gated sodium channels, insecticide resistance, mosquito control, resistance management, dengue vector, Brazil
MeSH terms
Mosquito Vectors, Aedes, Animals, Drug Resistance, Mosquito Control, Pyrethrins, Sodium Channels, Insect Proteins, Amino Acid Substitution, Mutation, Missense

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