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Use of colloidal gold surface plasmon resonance peak shift to infer affinity constants from the interactions between protein antigens and antibodies specific for single or multiple epitopes
Abstract
The surface plasmon resonance (SPR) wavelength of colloidal gold particles coated with a monoclonal antibody is red-shifted when the antibody interacts with its specific ligand. This shift results from the change in the refractive index of the particles as induced by ligand binding. This property is used to monitor in real-time the association and dissociation kinetics of the interaction in solution. The monitoring is performed in a clinical chemistry automated analyzer during a few minutes of incubation at 37 degrees C. Data treatment allows calculation of the affinity constant of the interaction. The SPR wavelength shift does not necessarily require agglutination or aggregation of the particles to occur since particles coated with one monoclonal antibody specific for a single epitope on the ligand can be used in the procedure. The affinity constants measured by this procedure correlate with those calculated from Scatchard plots or BIAcore data.
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Citations
Cited by
Haes, A. J., & Van Duyne, R. P. (2002) · Journal of the American Chemical Society · doi:10.1021/ja020393x · Cited by 2,057
Yonzon, C. R., Jeoung, E., Zou, S., Schatz, G. C., Mrksich, M., & Van Duyne, R. P. (2004) · Journal of the American Chemical Society · doi:10.1021/ja047118q · Cited by 494
Biomedical Applications of Plasmon Resonant Metal Nanoparticles
Liao, H., Nehl, C. L., & Hafner, J. H. (2006) · Nanomedicine · doi:10.2217/17435889.1.2.201 · Cited by 396
Optical properties of metallic nanoparticles: manipulating light, heat and forces at the nanoscale
Coronado, E. A., Encina, E. R., & Stefani, F. D. (2011) · Nanoscale · doi:10.1039/c1nr10788g · Cited by 284
Metadata
- Authors
- Englebienne, P.
- Venue
- The Analyst
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 1998
- Volume
- 123
- Issue
- 7
- Pages
- 1599-1603
- ISSN
- 0003-2654 (ISSN-L)
- 1364-5528
- OpenAlex
- W2000524950
- Cited by
- 294
- References
- 0
- FWCI
- 1.01
- Type
- journal article
- Language
- en
- Open access
- closed
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- Englebienne, P. (1998). Use of colloidal gold surface plasmon resonance peak shift to infer affinity constants from the interactions between protein antigens and antibodies specific for single or multiple epitopes. The Analyst. https://doi.org/10.1039/a804010i
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