The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Protein immobilization on carbon nanotubes via a two-step process of diimide-activated amidation
Abstract
Ferritin and bovine serum albumin (BSA) proteins are chemically bonded to nitrogen-doped multi-walled carbon nanotubes (CNx MWNTs) through a two-step process of diimide-activated amidation. First, carboxylated CNx MWNTs were activated by N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC), forming a stable active ester in the presence of N-hydroxysuccinimide (NHS). Second, the active ester was reacted with the amine groups on the proteins of ferritin or BSA, forming an amide bond between the CNx MWNTs and proteins. This two-step process avoids the intermolecular conjugation of proteins, and guarantees the uniform attachment of proteins on carbon nanotubes. TEM and AFM measurements clearly confirmed the successful attachment. This approach provides a universal and efficient method to attach biomolecules to carbon nanotubes at ambient conditions.
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Cites
Noncovalent sidewall functionalization of single-walled carbon nanotubes for protein immobilization
Chen, R. J., Zhang, Y., Wang, D., & Dai, H. (2001) · Journal of the American Chemical Society · doi:10.1021/ja010172b · Cited by 2,534
Cited by
Mohanty, N., & Berry, V. (2008) · Nano Letters · doi:10.1021/nl802412n · Cited by 1,208
The biocompatibility of carbon nanotubes
Smart, S. K., Cassady, A. I., Lu, G. Q., & Martin, D. J. (2006) · Carbon · doi:10.1016/j.carbon.2005.10.011 · Cited by 1,011
(1981) · PubMed · Cited by 235
Efficient anchorage of Pt clusters on N-doped carbon nanotubes and their catalytic activity
Lepró, X., Terrés, E., Vega-Cantú, Y., Rodríguez-Macías, F. J., Muramatsu, H., Kim, Y. A., Hayahsi, T., Endo, M., Torres R., M., & Terrones, M. (2008) · Chemical Physics Letters · doi:10.1016/j.cplett.2008.08.001 · Cited by 99
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Metadata
- Authors
- Jiang, K., Schadler, L. S., Siegel, R. W., Zhang, X., Zhang, H., & Terrones, M.
- Venue
- Journal of Materials Chemistry
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2004
- Volume
- 14
- Issue
- 1
- Pages
- 37
- ISSN
- 0959-9428 (ISSN-L)
- 1364-5501
- OpenAlex
- W2072923866
- Cited by
- 379
- References
- 1
- FWCI
- 5.72
- Type
- journal article
- Language
- en
- Open access
- closed
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- Jiang, K., Schadler, L. S., Siegel, R. W., Zhang, X., Zhang, H., & Terrones, M. (2004). Protein immobilization on carbon nanotubes via a two-step process of diimide-activated amidation. Journal of Materials Chemistry. https://doi.org/10.1039/b310359e
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