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Cited by 379

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Protein immobilization on carbon nanotubes via a two-step process of diimide-activated amidation

Jiang, Kuiyang, Schadler, Linda S., Siegel, Richard W., Zhang, Xinjie, Zhang, Haifeng, Terrones, Mauricio·2004·Journal of Materials Chemistry

Abstract

Ferritin and bovine serum albumin (BSA) proteins are chemically bonded to nitrogen-doped multi-walled carbon nanotubes (CNx MWNTs) through a two-step process of diimide-activated amidation. First, carboxylated CNx MWNTs were activated by N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC), forming a stable active ester in the presence of N-hydroxysuccinimide (NHS). Second, the active ester was reacted with the amine groups on the proteins of ferritin or BSA, forming an amide bond between the CNx MWNTs and proteins. This two-step process avoids the intermolecular conjugation of proteins, and guarantees the uniform attachment of proteins on carbon nanotubes. TEM and AFM measurements clearly confirmed the successful attachment. This approach provides a universal and efficient method to attach biomolecules to carbon nanotubes at ambient conditions.

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Metadata

Authors
Jiang, K., Schadler, L. S., Siegel, R. W., Zhang, X., Zhang, H., & Terrones, M.
Venue
Journal of Materials Chemistry
Published
2004
Volume
14
Issue
1
Pages
37
ISSN
  • 0959-9428 (ISSN-L)
  • 1364-5501
OpenAlex
W2072923866
Cited by
379
References
1
FWCI
5.72
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Protein immobilization on carbon nanotubes via a two-step process of diimide-activated amidation. https://app.cochat.ai/papers/10.1039/b310359e
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Jiang, K., Schadler, L. S., Siegel, R. W., Zhang, X., Zhang, H., & Terrones, M. (2004). Protein immobilization on carbon nanotubes via a two-step process of diimide-activated amidation. Journal of Materials Chemistry. https://doi.org/10.1039/b310359e

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