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Cited by 323

The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.

Dielectric spectroscopy in a micromachined flow cytometer: theoretical and practical considerations

Open access
Gawad, Shady, Cheung, Karen, Seger, Urban, Bertsch, Arnaud, Renaud, Philippe·2004·Lab on a Chip

Abstract

We propose a model to determine the influence of different cell properties, such as size, membrane capacitance and cytoplasm conductivity, on the impedance spectrum as measured in a microfabricated cytometer. A dielectric sphere of equivalent complex permittivity is used as a simplified model to describe a biological cell. The measurement takes place between a pair of facing microelectrodes in a microchannel filled with a saline solution. The model incorporates various cell parameters, such as dielectric properties, size and position in the channel. A 3D finite element model is used to evaluate the magnitude of the electric field in the channel and the resultant changes in charge densities at the measurement electrode boundaries as a cell flows past. The charge density is integrated on the electrode surface to determine the displacement current and the channel impedance for the computed frequency range. The complete impedance model combines the finite element model, the electrode-electrolyte interface impedance and stray impedance, which are measured from a real device. The modeled dielectric complex spectra for various cell parameters are discussed and a measurement strategy for cell discrimination with such a system is proposed. We finally discuss the amount of noise and measurement fluctuations of the sensor.

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Crossref · 10.1039/b313761a

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Metadata

Authors
Gawad, S., Cheung, K., Seger, U., Bertsch, A., & Renaud, P.
Venue
Lab on a Chip
Published
2004
Volume
4
Issue
3
Pages
241
ISSN
  • 1473-0189 (ISSN-L)
  • 1473-0197
OpenAlex
W2168420480
Cited by
323
References
15
FWCI
10.60
Type
journal article
Language
en
Open access
green

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