The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
The autofluorescence of plastic materials and chips measured under laser irradiation
Abstract
Plastic materials have the potential to substitute for glass substrates used in microfluidic and microTAS systems adding flexibility in materials' choices. Optical quality plastic materials with a low autofluorescence are crucial for optimal detection by fluorescence and laser induced fluorescence techniques. This paper summarizes a series of optical investigations on commercially available plastic chip materials (PMMA, COC, PC, PDMS) and chips made from those materials. Intrinsic optical constants of plastic materials-refractive index for bulk materials-determined by spectroscopic ellipsometry and transmission spectroscopy in the visible range are presented. The laser-induced autofluorescence of materials and chips was assessed at four laser wavelengths, namely, 403, 488, 532 and 633 nm. Considerable bleaching of the autofluorescence was observed under continuous laser illumination. Overall, the longer wavelength laser excitation sources yielded less autofluorescence. PDMS exhibited the least autofluorescence and was comparable to BoroFloat glass. In all cases, chips exhibited slightly higher autofluorescence than the raw plastic materials from which they had been made.
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Becker, H. (2002) · Talanta · doi:10.1016/s0039-9140(01)00594-x · Cited by 1,187
Disposable Microfluidic Devices: Fabrication, Function, and Application
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Nunes, P. S., Ohlsson, P. D., Ordeig, O., & Kutter, J. P. (2010) · Microfluidics and Nanofluidics · doi:10.1007/s10404-010-0605-4 · Cited by 434
Separation, characterization and identification of microplastics and nanoplastics in the environment
Fu, W., Min, J., Jiang, W., Li, Y., & Zhang, W. (2020) · Science of The Total Environment · doi:10.1016/j.scitotenv.2020.137561 · Cited by 361
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Metadata
- Authors
- Piruska, A., Nikcevic, I., Lee, S. H., Ahn, C., Heineman, W. R., Limbach, P. A., & Seliskar, C. J.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2005
- Volume
- 5
- Issue
- 12
- Pages
- 1348
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2157350971
- Cited by
- 431
- References
- 3
- FWCI
- 9.88
- Type
- journal article
- Language
- en
- Open access
- closed
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- Piruska, A., Nikcevic, I., Lee, S. H., Ahn, C., Heineman, W. R., Limbach, P. A., & Seliskar, C. J. (2005). The autofluorescence of plastic materials and chips measured under laser irradiation. Lab on a Chip. https://doi.org/10.1039/b508288a
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