The DOI matches its Crossref record.
High-throughput cell cycle synchronization using inertial forces in spiral microchannels
Abstract
Efficient synchronization and selection of cells at different stages of the cell replication cycle facilitates both fundamental research and development of cell cycle-targeted therapies. Current chemical-based synchronization methods are unfavorable as these can disrupt cell physiology and metabolism. Microfluidic systems developed for physical cell separation offer a potential alternative over conventional cell synchronization approaches. Here we introduce a spiral microfluidic device for cell cycle synchronization, using the combined effects of inertial forces and Dean drag force. By exploiting the relationship between cell diameter and cell cycle (DNA content/ploidy), we have successfully fractionated several asynchronous mammalian cell lines, as well as primary cells comprising bone marrow-derived human mesenchymal stem cells (hMSCs), into enriched subpopulations of G0/G1 (>85%), S, and G2/M phases. This level of cell cycle enrichment is comparable to existing microfluidic systems, but the throughput (∼ 15 × 10(6) cells per h) and viability (∼ 95%) of cells thus synchronized are significantly greater. Further, this platform provides rapid collection of synchronized cells or of diameter-sorted cells post-separation, to enable diverse applications in the study and manipulation of cell proliferation.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Fundamentals and applications of inertial microfluidics: a review
Zhang, J., Yan, S., Yuan, D., Alici, G., Nguyen, N.-T., Ebrahimi Warkiani, M., & Li, W. (2016) · Lab on a Chip · doi:10.1039/c5lc01159k · Cited by 1,017
Amini, H., Lee, W., & Di Carlo, D. (2014) · Lab on a Chip · doi:10.1039/c4lc00128a · Cited by 722
Self-citationSlanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells
Warkiani, M. E., Guan, G., Luan, K. B., Lee, W. C., Bhagat, A. A. S., Kant Chaudhuri, P., Tan, D. S.-W., Lim, W. T., Lee, S. C., Chen, P. C. Y., et al. (2014) · Lab Chip · doi:10.1039/c3lc50617g · Cited by 573
Self-citationHydrodynamic mechanisms of cell and particle trapping in microfluidics
Karimi, A., Yazdi, S., & Ardekani, A. M. (2013) · Biomicrofluidics · doi:10.1063/1.4799787 · Cited by 384
Guan, G., Wu, L., Bhagat, A. A., Li, Z., Chen, P. C. Y., Chao, S., Ong, C. J., & Han, J. (2013) · Scientific Reports · doi:10.1038/srep01475 · Cited by 279
Self-citation
Metadata
- Authors
- Lee, W. C., Bhagat, A. A. S., Huang, S., Van Vliet, K. J., Han, J., & Lim, C. T.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2011
- Volume
- 11
- Issue
- 7
- Pages
- 1359
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2164899082
- Cited by
- 164
- References
- 0
- FWCI
- 8.57
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: High-throughput cell cycle synchronization using inertial forces in spiral microchannels. https://app.cochat.ai/papers/10.1039/c0lc00579g
- The paper
- Lee, W. C., Bhagat, A. A. S., Huang, S., Van Vliet, K. J., Han, J., & Lim, C. T. (2011). High-throughput cell cycle synchronization using inertial forces in spiral microchannels. Lab on a Chip. https://doi.org/10.1039/c0lc00579g
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
