The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
In vitro 3D collective sprouting angiogenesis under orchestrated ANG-1 and VEGF gradients
Abstract
Sprouting angiogenesis requires a coordinated guidance from a variety of angiogenic factors. Here, we have developed a unique hydrogel incorporating microfluidic platform which mimics the physiological microenvironment in 3D under a precisely orchestrated gradient of soluble angiogenic factors, VEGF and ANG-1. The system enables the quantified investigation in chemotactic response of endothelial cells during the collective angiogenic sprouting process. While the presence of a VEGF gradient alone was sufficient in inducing a greater number of tip cells, addition of ANG-1 to the VEGF gradient enhanced the number of tip cells that are attached to collectively migrated stalk cells. The chemotactic response of tip cells attracted by the VEGF gradient and the stabilizing role of ANG-1 were morphologically investigated, elucidating the 3D co-operative migration of tip and stalk cells as well as their structures. We found that ANG-1 enhanced the connection of the stalk cells with the tip cells, and then the direct connection regulated the morphogenesis and/or life cycle of stalk cells.
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Cites
Angiogenesis in cancer and other diseases
Carmeliet, P., & Jain, R. K. (2000) · Nature · doi:10.1038/35025220 · Cited by 9,070
Vascular-specific growth factors and blood vessel formation
Yancopoulos, G. D., Davis, S., Gale, N. W., Rudge, J. S., Wiegand, S. J., & Holash, J. (2000) · Nature · doi:10.1038/35025215 · Cited by 3,699
Vernon, R. B., & Sage, E. H. (1999) · Microvascular Research · doi:10.1006/mvre.1998.2122 · Cited by 185
Cited by
Vascularization and Angiogenesis in Tissue Engineering: Beyond Creating Static Networks
Rouwkema, J., & Khademhosseini, A. (2016) · Trends in Biotechnology · doi:10.1016/j.tibtech.2016.03.002 · Cited by 651
Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels
Shin, Y., Han, S., Jeon, J. S., Yamamoto, K., Zervantonakis, I. K., Sudo, R., Kamm, R. D., & Chung, S. (2012) · Nature Protocols · doi:10.1038/nprot.2012.051 · Cited by 617
Self-citationLee, V. K., Lanzi, A. M., Ngo, H., Yoo, S.-S., Vincent, P. A., & Dai, G. (2014) · Cellular and Molecular Bioengineering · doi:10.1007/s12195-014-0340-0 · Cited by 342
Organ-on-a-chip platforms: a convergence of advanced materials, cells, and microscale technologies
Ahadian, S., Civitarese, R., Bannerman, D., Mohammadi, M. H., Lu, R., Wang, E., Davenport‐Huyer, L., Lai, B., Zhang, B., Zhao, Y., et al. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201700506 · Cited by 315
Micro Total Analysis Systems for Cell Biology and Biochemical Assays
Kovarik, M. L., Gach, P. C., Ornoff, D. M., Wang, Y., Balowski, J., Farrag, L., & Allbritton, N. L. (2012) · Analytical Chemistry · doi:10.1021/ac202611x · Cited by 270
Metadata
- Authors
- Shin, Y., Jeon, J. S., Han, S., Jung, G.-S., Shin, S., Lee, S.-H., Sudo, R., Kamm, R. D., & Chung, S.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2011
- Volume
- 11
- Issue
- 13
- Pages
- 2175
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W1997590060
- Cited by
- 166
- References
- 3
- FWCI
- 5.05
- Type
- journal article
- Language
- en
- Open access
- closed
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- Shin, Y., Jeon, J. S., Han, S., Jung, G.-S., Shin, S., Lee, S.-H., Sudo, R., Kamm, R. D., & Chung, S. (2011). In vitro 3D collective sprouting angiogenesis under orchestrated ANG-1 and VEGF gradients. Lab on a Chip. https://doi.org/10.1039/c1lc20039a
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