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A novel hydrogel matrix on gold surfaces in surface plasmon resonance sensors for fast and efficient covalent immobilization of ligands
Abstract
Gold surfaces intended for surface plasmon resonance sensors have been derivatized with a flexible matrix composed of carboxymethylated dextran to provide fast and efficient coupling of proteins and other ligands at low concentrations using the principles of ionic pre-concentration; these hydrophilic and protein compatible hydrogel surfaces allow increased immobilization capacity as compared to monolayer based coatings, for applications in biospecific interaction analysis.
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Citations
Cited by
Kinetic analysis of macromolecular interactions using surface plasmon resonance biosensors
Myszka, D. G. (1997) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(97)80157-7 · Cited by 552
van der Linden, R. H. J., Frenken, L. G. J., de Geus, B., Harmsen, M. M., Ruuls, R. C., Stok, W., de Ron, L., Wilson, S., Davis, P., & Verrips, C. T. (1999) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/s0167-4838(99)00030-8 · Cited by 471
Supported membranes on soft polymer cushions: fabrication, characterization and applications
Sackmann, E., & Tanaka, M. (2000) · Trends in Biotechnology · doi:10.1016/s0167-7799(99)01412-2 · Cited by 417
Schmitt, L., Ludwig, M., Gaub, H. E., & Tampé, R. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76863-9 · Cited by 171
Glycation of monoclonal antibodies impairs their ability to bind antigen
KENNEDY, D. M., SKILLBN, A. W., & SELF, C. H. (2008) · Clinical and Experimental Immunology · doi:10.1111/j.1365-2249.1994.tb06133.x · Cited by 74
Metadata
- Authors
- Löfås, S., & Johnsson, B.
- Venue
- J. Chem. Soc., Chem. Commun.
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 1990
- Issue
- 21
- Pages
- 1526-1528
- ISSN
- 0022-4936 (ISSN-L)
- OpenAlex
- W2164929179
- Cited by
- 766
- References
- 0
- FWCI
- 5.27
- Type
- journal article
- Language
- en
- Open access
- closed
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- Löfås, S., & Johnsson, B. (1990). A novel hydrogel matrix on gold surfaces in surface plasmon resonance sensors for fast and efficient covalent immobilization of ligands. J. Chem. Soc., Chem. Commun. https://doi.org/10.1039/c39900001526
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
