The DOI matches its Crossref record; 49 of the 52 references it cites were found in a registry or an index, and 1 are not checked yet.
Cell patterning with a heptagon acoustic tweezer – application in neurite guidance
Open accessAbstract
Accurate control over positioning of cells is a highly desirable feature in tissue engineering applications since it allows, for example, population of substrates in a controlled fashion, rather than relying on random seeding. Current methods to achieve a differential distribution of cells mostly use passive patterning methods to change chemical, mechanical or topographic properties of surfaces, making areas differentially permissive to the adhesion of cells. However, these methods have no ad hoc control over the actual deposition of cells. Direct patterning methods like bioprinting offer good control over cell position, but require sophisticated instrumentation and are often cost- and time-intensive. Here, we present a novel electronically controlled method of generating dynamic cell patterns by acoustic trapping of cells at a user-determined position, with a heptagonal acoustic tweezer device. We demonstrate the capability of the device to create complex patterns of cells using the device's ability to re-position acoustic traps by using a phase shift in the acoustic wave, and by switching the configuration of active piezoelectric transducers. Furthermore, we show that by arranging Schwann cells from neonatal rats in a linear pattern we are able to create Bands of Büngner-like structures on a non-structured surface and demonstrate that these features are able to guide neurite outgrowth from neonatal rat dorsal root ganglia.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Furthermore, the acoustofluidic technology provides excellent biocompatibility, and has been demonstrated to pattern and grow nerve cells such as Schwann cells.40”
From Acoustofluidic assembly of 3D neurospheroids to model Alzheimer's disease (2020) · cites this paper as [40]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
NIH Image to ImageJ: 25 years of image analysis
Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012) · Nature Methods · doi:10.1038/nmeth.2089 · Cited by 64,638
Geometric Control of Cell Life and Death
Chen, C. S., Mrksich, M., Huang, S., Whitesides, G. M., & Ingber, D. E. (1997) · Science · doi:10.1126/science.276.5317.1425 · Cited by 4,710
Cell Movement Is Guided by the Rigidity of the Substrate
Lo, C.-M., Wang, H.-B., Dembo, M., & Wang, Y.-L. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76279-5 · Cited by 3,289
Principles of Tissue Engineering
(2014) · Elsevier eBooks · doi:10.1016/c2011-0-07193-4 · Cited by 1,913
Engineering cell shape and function
Singhvi, R., Kumar, A., Lopez, G. P., Stephanopoulos, G. N., Wang, D. I. C., Whitesides, G. M., & Ingber, D. E. (1994) · Science · doi:10.1126/science.8171320 · Cited by 1,453
Rezakhaniha, R., Agianniotis, A., Schrauwen, J. T. C., Griffa, A., Sage, D., Bouten, C. V. C., van de Vosse, F. N., Unser, M. A., & Stergiopulos, N. (2011) · Biomechanics and Modeling in Mechanobiology · doi:10.1007/s10237-011-0325-z · Cited by 1,104
Inkjet printing of viable mammalian cells
Xu, T., Jin, J., Gregory, C., Hickman, J. J., & Boland, T. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.04.011 · Cited by 1,036
ELECTRICAL FORCES FOR MICROSCALE CELL MANIPULATION
Joel Voldman, B. (2006) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.8.061505.095739 · Cited by 913
(2009) · doi:10.1007/978-3-540-29678-2 · Cited by 900
Acoustic tweezers: patterning cells and microparticles using standing surface acoustic waves (SSAW)
Shi, J., Ahmed, D., Mao, X., Lin, S. S., Lawit, A., & Huang, T. J. (2009) · Lab on a Chip · doi:10.1039/b910595f · Cited by 724
Contributing factors to poor functional recovery after delayed nerve repair: prolonged denervation
Fu, S., & Gordon, T. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-05-03886.1995 · Cited by 701
Chiu, D. T., Jeon, N. L., Huang, S., Kane, R. S., Wargo, C. J., Choi, I. S., Ingber, D. E., & Whitesides, G. M. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.040562297 · Cited by 525
Repairing injured peripheral nerves: Bridging the gap
Deumens, R., Bozkurt, A., Meek, M. F., Marcus, M. A. E., Joosten, E. A. J., Weis, J., & Brook, G. A. (2010) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2010.10.002 · Cited by 508
EphB Signaling Directs Peripheral Nerve Regeneration through Sox2-Dependent Schwann Cell Sorting
Parrinello, S., Napoli, I., Ribeiro, S., Wingfield-Digby, P., Fedorova, M., Parkinson, D. B., Doddrell, R. D. S., Nakayama, M., Adams, R. H., & Lloyd, A. C. (2010) · Cell · doi:10.1016/j.cell.2010.08.039 · Cited by 486
Schwann cell processes guide regeneration of peripheral axons
Son, Y., & Thompson, W. J. (1995) · Neuron · doi:10.1016/0896-6273(95)90246-5 · Cited by 444
Biological Laser Printing: A Novel Technique for Creating Heterogeneous 3-dimensional Cell Patterns
Barron, J. A., Wu, P., Ladouceur, H. D., & Ringeisen, B. R. (2004) · Biomedical Microdevices · doi:10.1023/b:bmmd.0000031751.67267.9f · Cited by 437
Using electroactive substrates to pattern the attachment of two different cell populations
Yousaf, M. N., Houseman, B. T., & Mrksich, M. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.101112898 · Cited by 351
Laser-guided direct writing for three-dimensional tissue engineering.
Nahmias, Y., Schwartz, R., Verfaillie, C., & Odde, D. (2005) · Biotechnology and Bioengineering · doi:10.1002/bit.20585 · Cited by 274
Simple Approach to Micropattern Cells on Common Culture Substrates by Tuning Substrate Wettability
Tan, J. L., Liu, W. F., Nelson, C. M., Raghavan, S., & Chen, C. S. (2004) · Tissue Engineering · doi:10.1089/1076327041348365 · Cited by 236
Engineered neural tissue for peripheral nerve repair
Georgiou, M., Bunting, S. C. J., Davies, H. A., Loughlin, J., Golding, J. P., & Phillips, J. B. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.06.025 · Cited by 224
Strategies for inducing the formation of bands of Büngner in peripheral nerve regeneration
Ribeiro‐Resende, V. T., Koenig, B., Nichterwitz, S., Oberhoffner, S., & Schlosshauer, B. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.07.007 · Cited by 215
Pariente, J.-L., Kim, B., & Atala, A. (2001) · Journal of Biomedical Materials Research · doi:10.1002/1097-4636(200104)55:1 · Cited by 204
Binding strength and activation state of single fibrinogen-integrin pairs on living cells
Litvinov, R. I., Shuman, H., BENNETT, J. S., & Weisel, J. W. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.112194999 · Cited by 191
Bozkurt, A., Deumens, R., Beckmann, C., Damink, L. O., Schügner, F., Heschel, I., Sellhaus, B., Weis, J., Jahnen‐Dechent, W., Brook, G. A., et al. (2008) · Biomaterials · doi:10.1016/j.biomaterials.2008.09.017 · Cited by 176
Neural Tissue Engineering: A Self-Organizing Collagen Guidance Conduit
Phillips, J. B., Bunting, S. C. J., Hall, S. M., & Brown, R. A. (2005) · Tissue Engineering · doi:10.1089/ten.2005.11.1611 · Cited by 141
Electric field-induced astrocyte alignment directs neurite outgrowth
ALEXANDER, J. K., Fuss, B., & Colello, R. J. (2006) · Neuron Glia Biology · doi:10.1017/s1740925x0600010x · Cited by 136
Rapid and Serial Quantification of Adhesion Forces of Yeast and Mammalian Cells
Potthoff, E., Guillaume-Gentil, O. J., Ossola, D., Polesel‐Maris, J., LeibundGut‐Landmann, S., Zambelli, T., & Vorholt, J. A. (2012) · PLoS ONE · doi:10.1371/journal.pone.0052712 · Cited by 132
Miller, C., Jeftinija, S., & Mallapragada, S. K. (2001) · Tissue Engineering · doi:10.1089/107632701753337663 · Cited by 130
Fernández‐Valle, C., Gorman, D., Gomez, A. M., & Bunge, M. B. (1997) · Journal of Neuroscience · doi:10.1523/jneurosci.17-01-00241.1997 · Cited by 122
Schmalenberg, K. E., & Uhrich, K. E. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.04.046 · Cited by 107
Negative dielectrophoretic patterning with different cell types
Suzuki, M., Yasukawa, T., Shiku, H., & Matsue, T. (2008) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2008.06.051 · Cited by 93
Schwann Cell Response to Micropatterned Laminin Surfaces
Thompson, D. M., & Buettner, H. M. (2001) · Tissue Engineering · doi:10.1089/10763270152044125 · Cited by 76
Bernassau, A. L., Glynne‐Jones, P., Gesellchen, F., Riehle, M. O., Hill, M., & Cumming, D. R. S. (2013) · Ultrasonics · doi:10.1016/j.ultras.2013.04.019 · Cited by 72
Self-citationThe Biology of Chronically Denervated Schwann Cells
HALL, S. M. (1999) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1999.tb08584.x · Cited by 71
Oriented Schwann Cell Monolayers for Directed Neurite Outgrowth
Thompson, D. M., & Buettner, H. M. (2004) · Annals of Biomedical Engineering · doi:10.1114/b:abme.0000036648.68804.e7 · Cited by 66
Garvin, K. A., Hocking, D. C., & Dalecki, D. (2010) · Ultrasound in Medicine & Biology · doi:10.1016/j.ultrasmedbio.2010.08.007 · Cited by 66
Mechanical Gradient Cues for Guided Cell Motility and Control of Cell Behavior on Uniform Substrates
Cortese, B., Gigli, G., & Riehle, M. O. (2009) · Advanced Functional Materials · doi:10.1002/adfm.200900918 · Cited by 65
Self-citationDynamic cell adhesion and migration on nanoscale grooved substrates
Lamers, E., Riet, J. T., Domanski, M., Lüttge, R., Figdor, C. G., Gardeniers, J., Walboomers, X. F., & Jansen, J. (2012) · European Cells and Materials · doi:10.22203/ecm.v023a14 · Cited by 64
Proprioception: Effect of Neurological Disease
Cole, J. (2008) · Springer eBooks · doi:10.1007/978-3-540-29678-2_4824 · Cited by 64
A Biodegradable and Biocompatible Regular Nanopattern for Large‐Scale Selective Cell Growth
Csáderová, L., Martines, E., Seunarine, K., Gadegaard, N., Wilkinson, C. D. W., & Riehle, M. O. (2010) · Small · doi:10.1002/smll.201000193 · Cited by 50
Self-citationKhuong, H. T., & Midha, R. (2012) · Current Neurology and Neuroscience Reports · doi:10.1007/s11910-012-0322-3 · Cited by 39
Yong-Guang, Z., Sheng, Q., Qi, F., Hu, X., Zhao, W. G., Wang, Y., Lan, L.-F., Huang, J., & Luo, Z.-J. (2013) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-013-4917-2 · Cited by 39
Use of Force Spectroscopy to Investigate the Adhesion of Living Adherent Cells
Weder, G., Blondiaux, N., Giazzon, M., Matthey, N., Klein, M. J. K., Pugin, R., Heinzelmann, H., & Liley, M. (2010) · Langmuir · doi:10.1021/la904526u · Cited by 34
In Situ Tissue Engineering Using Magnetically Guided Three-Dimensional Cell Patterning
Grogan, S. P., Pauli, C., Chen, P., Du, J., Chung, C. B., Kong, S. D., Colwell, C. W., Lotz, M., Jin, S., & DʼLima, D. D. (2012) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2011.0525 · Cited by 34
Laser-guided cell micropatterning system
Pirlo, R. K., Ma, Z., Sweeney, A. J., Liu, H., Yun, J. X., Peng, X., Yuan, X., Guo, G. X., & Gao, B. Z. (2011) · Review of Scientific Instruments · doi:10.1063/1.3529919 · Cited by 28
Direct patterning of mammalian cells in an ultrasonic heptagon stencil
Bernassau, A. L., Gesellchen, F., MacPherson, P., Riehle, M. O., & Cumming, D. R. S. (2012) · Biomedical Microdevices · doi:10.1007/s10544-012-9633-z · Cited by 27
Self-citationGherardini, L. M., Radel, S., Sielemann, S., Doblhoff‐Dier, O., Gröschl, M., BENES, E., & McLoughlin, A. J. (2001) · Bioseparation · doi:10.1023/a:1016311410219 · Cited by 27
Photocontrol of Cell Adhesion and Proliferation by a Photoinduced Cationic Polymer Surface ¶
Nakayama, Y., Furumoto, A., Kidoaki, S., & Matsuda, T. (2003) · Photochemistry and Photobiology · doi:10.1562/0031-8655(2003)0770480pocaap2.0.co2 · Cited by 24
Two-dimensional manipulation of micro particles by acoustic radiation pressure in a heptagon cell
Bernassau, A. L., Ong, C.-K., Ma, Y., MacPherson, P., Courtney, C. R. P., Riehle, M. O., Drinkwater, B. W., & Cumming, D. R. S. (2011) · IEEE Transactions on Ultrasonics Ferroelectrics and Frequency Control · doi:10.1109/tuffc.2011.2062 · Cited by 24
Self-citationThe Antiapoptotic Effect of Low-dose UVB Irradiation in NIH3T3 Cells Involves Caspase Inhibition¶
Ibuki, Y., & Gotô, R. (2003) · Pure Amsterdam UMC · doi:10.1562/0031-8655(2003)077 · Cited by 1
Research works printed but not linked: 1
- Zhang, 2013, J. Mater. Sci.: Mater. Med., 24, 1767
Cited by
Sorted by citations
Three-dimensional manipulation of single cells using surface acoustic waves
Guo, F., Mao, Z., Chen, Y., Xie, Z., Lata, J. P., Li, P., Ren, L., Liu, J., Yang, J. H., Dao, M., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1524813113 · Cited by 612
Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning
Armstrong, J. P. K., Puetzer, J. L., Serio, A., Guex, A. G., Kapnisi, M., Breant, A., Zong, Y., Assal, V., Skaalure, S. C., King, O., et al. (2018) · Advanced Materials · doi:10.1002/adma.201802649 · Cited by 230
Meng, L., Cai, F., Li, F., Zhou, W., Niu, L., & Zheng, H. (2019) · Journal of Physics D Applied Physics · doi:10.1088/1361-6463/ab16b5 · Cited by 195
Tian, L., Martin, N., Bassindale, P. G., Patil, A. J., Li, M., Barnes, A. C., Drinkwater, B. W., & Mann, S. (2016) · Nature Communications · doi:10.1038/ncomms13068 · Cited by 172
Enriching Nanoparticles via Acoustofluidics
Mao, Z., Li, P., Wu, M., Bachman, H., Mesyngier, N., Guo, X., Liu, S., Costanzo, F., & Huang, T. J. (2017) · ACS Nano · doi:10.1021/acsnano.6b06784 · Cited by 169
Metadata
- Authors
- Gesellchen, F., Bernassau, A. L., Déjardin, T., Cumming, D. R. S., & Riehle, M. O.
- Affiliations
- University of Glasgow (GB)
- Corresponding author
- Riehle, M. O.
- Venue
- Lab Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2014
- Volume
- 14
- Issue
- 13
- Pages
- 2266-2275
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2104860111
- Cited by
- 98
- References
- 52
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 6 · 2023: 9 · 2022: 9 · 2021: 8 · 2020: 14 · 2019: 11 · 2018: 9 · 2017: 11 · 2016: 9 · 2015: 5 · 2014: 2
- FWCI
- 4.67
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- Lab on a Chip (publishedVersion, cc-by)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (acceptedVersion)
- Funders
- Engineering and Physical Sciences Research Council (EP/G011494/1)
- University Of Glasgow (Lord Kelvin Adam Smith Fellowship in Sensor System)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- cell patterning, neurite guidance, Schwann cells, dorsal root ganglia, piezoelectric transducers, cell positioning, tissue engineering
- MeSH terms
- Animals, Dendrites, Ganglia, Spinal, Schwann Cells, Sound, Rats, Sprague-Dawley, Coculture Techniques, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
