Unambiguous identification and discovery of bacterial siderophores by direct injection 21 Tesla Fourier transform ion cyclotron resonance mass spectrometry
Abstract
Under iron-limiting conditions, bacteria produce low molecular mass Fe(iii) binding molecules known as siderophores to sequester the Fe(iii), along with other elements, increasing their bioavailability. Siderophores are thought to influence iron cycling and biogeochemistry in both marine and terrestrial ecosystems and hence the need for rapid, confident characterization of these compounds has increased. In this study, the type of siderophores produced by two marine bacterial species, Synechococcus sp. PCC 7002 and Vibrio cyclitrophicus 1F53, were characterized by use of a newly developed 21 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FTICR MS) with direct injection electrospray ionization. This technique allowed for the rapid detection of synechobactins from Synechococcus sp. PCC 7002 as well as amphibactins from Vibrio cyclitrophicus 1F53 based on high mass accuracy and resolution allowing for observation of specific Fe isotopes and isotopic fine structure enabling highly confident identification of these siderophores. When combined with molecular network analysis two new amphibactins were discovered and verified by tandem MS. These results show that high-field FTICR MS is a powerful technique that will greatly improve the ability to rapidly identify and discover metal binding species in the environment.
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Previous techniques for elucidating metal-binding relied on properties of the metal-adduct itself, including metal-specific isotopic signatures, mass defect/Kendrick analysis, or hyperfine splitting isotope patterns.
“Previous techniques for elucidating metal-binding have relied on properties of the metal-adduct itself, including the presence of metal-specific isotopic signatures,87,88 mass defect/Kendrick analysis,89 or analysis of hyperfine splitting isotope patterns.90”
From Connecting metabolome and phenotype: recent advances in functional metabolomics tools for the identification of bioactive natural products (2024) · cites this paper as [90]
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- Authors
- Walker, L. R., Tfaily, M. M., Shaw, J. B., Hess, N. J., Paša-Tolić, L., & Koppenaal, D. W.
- Affiliations
- Pacific Northwest National Laboratory (US)
- Corresponding author
- Koppenaal, David W.
- Venue
- Metallomics
- Publisher
- Oxford University Press (OUP)
- Published
- 2017
- Volume
- 9
- Issue
- 1
- Pages
- 82-92
- ISSN
- 1756-5901 (ISSN-L)
- 1756-591X
- OpenAlex
- W2558348433
- Cited by
- 31
- References
- 39
- Citations by year
- 2025: 4 · 2024: 5 · 2023: 2 · 2022: 1 · 2021: 5 · 2020: 4 · 2019: 6 · 2018: 3 · 2017: 1
- FWCI
- 1.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Metallomics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Massachusetts Institute of Technology
- Office of Science
- Pacific Northwest National Laboratory
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Keywords
- bacterial siderophores, iron acquisition, Synechococcus sp. PCC 7002, Vibrio cyclitrophicus, FT-ICR mass spectrometry, iron isotopes, molecular network analysis, marine bacteria
- MeSH terms
- Humans, Iron, Vibrio, Vibrio Infections, Siderophores, Cyclotrons, Spectroscopy, Fourier Transform Infrared, Spectrometry, Mass, Electrospray Ionization, Synechococcus
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