Regenerating infected bone defects with osteocompatible microspheres possessing antibacterial activity
Abstract
Treatment of infected bone defects still remains a formidable clinical challenge, and the design of bone implants with both anti-bacterial activity and -osteogenesis effects is nowadays regarded as a powerful strategy for infection control and bone healing. In the present study, bioresorbable porous-structured microspheres were fabricated from an amphiphilic block copolymer composed of poly(l-lactide) and poly(ethyl glycol) blocks. After being surface coated with mussel-inspired polydopamine, the microspheres were loaded with nanosilver via the reduction of silver nitrate and apatite via biomineralization in sequence. At optimized loading amounts, the nanosilver-loaded microspheres showed no unfavorable effects on the proliferation and differentiation of bone marrow mesenchymal stem cells despite preserving strong antibacterial activity in in vitro evaluations. For the critical-sized defects (φ = 8 mm) in the rat cranium that was pre-infected with Staphylococcus aureus, the filling of the dual-purpose microspheres demonstrated an effective way to kill bacteria in vivo, and in the meantime, it promoted new bone formation efficiently alongside the degradation of microspheres. Thus, the results suggested that bioresorbable microspheres with both osteoconductive and antibacterial activities were a good choice for treating infected bone defects.
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Developed dual-purpose microspheres were biodegradable and potentially injectable, with both osteoconductive and antibacterial activities for treating infected bone defects.
“These developed dual-purpose microspheres were biodegradable and potentially injectable, with both osteoconductive and antibacterial activities for treating infected bone defects.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [70]
Dual-purpose microspheres stimulated new bone formation efficiently as observed by higher expression of osteogenic genes OPN (~5%), OCN (~4%), and collagen type-1 (~8%) at day 14.
“and at the same time stimulating new bone formation efficiently as observed by the higher expression of osteogenic genes OPN (~5%), OCN (~4%), and collagen type-1 (~8%) at day 14.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [70]
When bone defects (φ = 8 mm) were initiated using S. aureus bacteria, dual-purpose microspheres proved effective for killing bacteria in the rat cranium.
“when bone defects (ϕ = 8 mm) were initiated using S. aureus bacteria, the dual-purpose microspheres proved an effective approach for killing bacteria in the rat cranium”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [70]
Synthesized microspheres showed no negative effects on the differentiation of bone marrow mesenchymal stem cells regardless of strong antibacterial activity in in-vitro studies.
“Interestingly, these synthesized microspheres showed no negative effects on the differentiation of bone marrow mesenchymal stem cells regardless of strong antibacterial activity as seen in in-vitro studies.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [70]
Cites
Mussel‐Inspired Polydopamine Coating as a Universal Route to Hydroxyapatite Crystallization
Ryu, J., Ku, S. H., Lee, H., & Park, C. B. (2010) · Advanced Functional Materials · doi:10.1002/adfm.200902347 · Cited by 778 · see citing works
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Kaushik, N., Nhat Nguyen, L., Kim, J. H., Choi, E. H., & Kumar Kaushik, N. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21186544 · Cited by 76 · see citing works
“fabricated bioresorbable porous-structured microspheres from an amphiphilic block copolymer consisting of PLLA and poly (ethyl glycol) blocks [70].”
Background
- Authors
- Wei, P.-F., Yuan, Z.-Y., Jing, W., Guan, B.-B., Liu, Z.-H., Zhang, X., Mao, J.-P., Chen, D.-F., Cai, Q., & Yang, X.-P.
- Affiliations
- State Key Laboratory of Organic-Inorganic Composites; Beijing Laboratory of Biomedical Materials; Beijing University of Chemical Technology
- Tianjin Medical University General Hospital (CN)
- Tianjin Medical University (CN)
- Beijing Jishuitan Hospital (CN)
- Laboratory of Bone Tissue Engineering
- State Key Laboratory of Organic-Inorganic Composite Materials (CN)
- Corresponding author
- Cai, Qing
- Venue
- Biomaterials Science
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2019
- Volume
- 7
- Issue
- 1
- Pages
- 272-286
- ISSN
- 2047-4830 (ISSN-L)
- 2047-4849
- OpenAlex
- W2900262171
- Cited by
- 27
- References
- 1
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 5 · 2022: 7 · 2021: 2 · 2020: 8 · 2019: 2
- FWCI
- 1.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (51473016, 51873018)
- Natural Science Foundation of Beijing Municipality (7182068, 7161001)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Surgery › Orthopedic Infections and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Keywords
- infected bone defects, antibacterial activity, silver nanoparticles, polydopamine coating, mesenchymal stem cells, Staphylococcus aureus, critical-size calvarial defect, bone regeneration, osteoconduction
- MeSH terms
- Animals, Anti-Bacterial Agents, Apatites, Bone Regeneration, Cell Line, Indoles, Microspheres, Osteogenesis, Polymers, Silver, Skull, Staphylococcal Infections, Staphylococcus aureus, Rats, Sprague-Dawley, Coated Materials, Biocompatible, Rats
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