The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Ferroxidase activity of ferritin: effects of pH, buffer and Fe(II) and Fe(III) concentrations on Fe(II) autoxidation and ferroxidation
Open accessAbstract
It is widely accepted that iron deposition in the iron storage protein ferritin in vitro involves Fe(II) oxidation, and that ferritin facilitates this oxidation at a ferroxidase site on the protein. However, these views have recently been questioned, with the protein ferroxidase activity instead being attributed to autoxidation from the buffer alone. Ligand exchange between another protein with ferroxidase activity and ferritin has been proposed as an alternative mechanism for iron incorporation into ferritin. In the present work, a pH stat apparatus is used to eliminate the influence of buffers on iron(II) oxidation. Here we show that the recent experiments questioning the ferroxidase activity of ferritin were flawed by inadequate pH control, that buffers actually retard rather than facilitate iron(II) oxidation, and that horse spleen ferritin has ferroxidase activity when measured under proper experimental conditions. Furthermore, high pH (7.0), a high Fe(II) concentration and the presence of Fe(III) all favour Fe(II) autoxidation in the presence or absence of ferritin.
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“The iron in the central cavity is maintained as small crystalline particles in its ferric Fe3+ form due to the ferroxidase activity of FTH1 (Hentze et al., 1986; Yang and Chasteen, 1999).”
From The Role of Iron Regulation in Immunometabolism and Immune-Related Disease (2019) · cites this paper as Yang and Chasteen, 1999
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Citations
Cites
Hydrogen Ion Buffers for Biological Research*
Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S., & Singh, R. M. M. (1966) · Biochemistry · doi:10.1021/bi00866a011 · Cited by 2,762
The ferritins: molecular properties, iron storage function and cellular regulation
Harrison, P. M., & Arosio, P. (1996) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(96)00022-9 · Cited by 2,667
Identification of the ferroxidase centre in ferritin
Lawson, D. M., Treffry, A., Artymiuk, P. J., Harrison, P. M., Yewdall, S. J., Luzzago, A., Cesareni, G., Levi, S., & Arosio, P. (1989) · FEBS Letters · doi:10.1016/0014-5793(89)81040-3 · Cited by 312
Analysis of the effects of changes in rate and rhythm upon electrical activity in the heart
Boyet, M. R., & Jewell, B. R. (1981) · Progress in Biophysics and Molecular Biology · doi:10.1016/0079-6107(81)90003-1 · Cited by 279
(1995) · General Pharmacology The Vascular System · doi:10.1016/s0306-3623(99)80009-3 · Cited by 12
Cited by
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The Role of Iron Regulation in Immunometabolism and Immune-Related Disease
Cronin, S. J. F., Woolf, C. J., Weiss, G., & Penninger, J. M. (2019) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2019.00116 · Cited by 299
Overexpression of Wild Type and Mutated Human Ferritin H-chain in HeLa Cells
Cozzi, A., Corsi, B., Levi, S., Santambrogio, P., Albertini, A., & Arosio, P. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m003797200 · Cited by 242
The Ferroxidase Activity of Yeast Frataxin
Park, S., Gakh, O., Mooney, S. M., & Isaya, G. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m206711200 · Cited by 88
Unveiling ferroptosis as a promising therapeutic avenue for colorectal cancer and colitis treatment
Kao, A. T., Cabanlong, C. V., Padilla, K., & Xue, X. (2024) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2024.05.025 · Cited by 30
Metadata
- Authors
- YANG, X., & CHASTEEN, N. D.
- Affiliations
- University of New Hampshire (US)
- ORCID
- YANG, Xiaoke
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1999-03-08
- Volume
- 338
- Issue
- 3
- Pages
- 615-618
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1220094
- OpenAlex
- W2043595508
- Cited by
- 52
- References
- 5
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 4 · 2023: 2 · 2022: 1 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 4
- FWCI
- 1.61
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (R37 GM20194, R37GM020194)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Hemoglobin structure and function
- Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
- Keywords
- ferritin, ferroxidase activity, iron(II) oxidation, iron storage, pH effect, buffering effect, horse spleen ferritin, iron incorporation, ligand exchange
- MeSH terms
- Animals, Buffers, Ceruloplasmin, Ferritins, Horses, Hydrogen-Ion Concentration, Iron, Oxidation-Reduction
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