Nuclear proteins as gene-transfer vectors
Abstract
An interest in nuclear proteins as possible gene vectors arose as early as 1988. Nuclear proteins possess important features, such as DNA condensing capacity and the presence of NLSs (nuclear localization signals), which are thought to be perfect tools to combine cellular and nuclear uptake of DNA. The NLS of the proteins might support the intracellular trafficking of the complexes, whereas their condensing capacity enables DNase stability. Nuclear proteins, histones and also non-histones, such as the high-mobility-group proteins, have been successfully employed in in vitro gene transfer by a variety of workers over the last decade. In the pertinent published data, almost all nuclear proteins tested showed gene-transfer activity. The degree of activity of the individual nuclear proteins tested varied according to sequential and structural differences of the proteins, discriminating their DNA binding behaviour, but reported transfection efficiency differences are inconsistent. They do not reveal a logical pattern, such as a correlation with their DNA affinity. The differences in transfection efficiency of certain nuclear proteins reported by different authors could be based on a lot of factors, including the source or method of protein preparation, the cells used, the media or the transfection additives, such as lysosomotropic agents. The variances are quite random, which suggests an involvement of physicochemical parameters of the nuclear protein-DNA complexes in the efficiency of gene expression. Physicochemical features of the complexes are also determined by factors such as transfection additives (e.g. Ca(2+)). The present review therefore includes, in addition to an overview about the nuclear proteins used in gene-transfer experiments, some assumptions about the mechanism of the cellular uptake of nuclear protein-DNA complexes and their translocation to the nucleus.
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NLSs have been introduced into vectors to facilitate transportation to nucleus.
“Inspired by the active transportation process of proteins to nucleus, nuclear localization sequences (NLSs) have been introduced into the vectors to facilitate transportation to nucleus [195–197].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [195]
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Won, Y.-W., Lim, K. S., & Kim, Y. (2011) · Journal of Controlled Release · doi:10.1016/j.jconrel.2011.01.013 · Cited by 75 · see citing works
A comprehensive review on histone-mediated transfection for gene therapy
Han, H., Yang, J., Chen, W., Li, Q., Yang, Y., & Li, Q. (2018) · Biotechnology Advances · doi:10.1016/j.biotechadv.2018.11.009 · Cited by 25 · see citing works
Growth enhancement of shrimp (Litopenaeus schmitti) after transfer of tilapia growth hormone gene
Arenal, A., Pimentel, R., Pimentel, E., Martín, L., Santiesteban, D., Franco, R., & Aleström, P. (2008) · Biotechnology Letters · doi:10.1007/s10529-008-9636-2 · Cited by 20 · see citing works
“The NLS has been used to increase the non-viral gene transfer in high vertebrates (Haberland and Böttger 2005).”
MethodInteraction of DNA/nuclear protein/polycation and the terplexes for gene delivery
Shen, Y., Peng, H., Pan, S., Feng, M., Wen, Y., Deng, J., Luo, X., & Wu, C. (2009) · Nanotechnology · doi:10.1088/0957-4484/21/4/045102 · Cited by 19 · see citing works
High level expression of proteins using sequences from the ferritin heavy chain gene locus
Prentice, H. L., Tonkin, C. J., Caamano, L., & Sisk, W. P. (2006) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2006.09.021 · Cited by 15 · see citing works
Zarei, H., Malaekeh‐Nikouei, B., Ramezani, M., & Soltani, F. (2021) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2021.102422 · Cited by 7 · see citing works
Structural appearance of linker histone H1/siRNA complexes
Haberland, A., Zaitsev, S. V., Waldöfner, N., Erdmann, B., Böttger, M., & Henke, W. (2008) · Molecular Biology Reports · doi:10.1007/s11033-008-9282-8 · Cited by 6 · see citing works
“Because of the many positive features H1 possess (for review see [ 25 ]), it would make a useful vector, if appropriate.”
BackgroundResultInfluentialBocharova, T. N., Smirnova, E. A., & Volodin, A. A. (2011) · Biopolymers · doi:10.1002/bip.22010 · Cited by 5 · see citing works
Nucleocytoplasmic Transport of Plasmid DNA: A Perilous Journey from the Cytoplasm to the Nucleus
Lechardeur, D., & Lukács, G. L. (2006) · Human Gene Therapy · doi:10.1089/hum.2006.17.ft-238 · Cited by 4 · see citing works
A novel DNA-binding protein fromCampylobacter jejunibacteriophage NCTC12673
Arutyunov, D., & Szymanski, C. M. (2015) · FEMS Microbiology Letters · doi:10.1093/femsle/fnv160 · Cited by 2 · see citing works
Biological Applications of Polymeric Nanoparticles
Rangelov, S., & Pispas, A. (2013) · CRC Press eBooks · doi:10.1201/b15390-6 · Cited by 1 · see citing works
Bimodal character of the solubility isotherm of histone H1 complexes with short oligonucleotides
Bocharova, T. N., Kvitko, N. P., Smirnova, E. A., & Volodin, A. A. (2011) · Molecular Biology · doi:10.1134/s0026893311020026 · Cited by 1 · see citing works
Wong, S. Y. (2008) · eCommons (Cornell University)
Nanoparticles: Biological Applications
Rangelov, S., & Pispas, S. (2017) · CRC Press eBooks · doi:10.1081/e-ebppc-120052544
Nanoparticles: Biological Applications
Rangelov, S., & Pispas, S. (2015) · Taylor & Francis eBooks · doi:10.1081/e-ebpp-120052544
Соловьева, В. В., Кудряшова, Н. В., & Rizvanov, A. A. (2011) · Genes and Cells
- Authors
- Haberland, A., & Böttger, M.
- Affiliations
- Freie Universität Berlin (DE)
- Max Delbrück Center (DE)
- Venue
- Biotechnology and Applied Biochemistry
- Publisher
- Wiley
- Published
- 2005-10
- Volume
- 42
- Issue
- 2
- Pages
- 97-106
- ISSN
- 0885-4513 (ISSN-L)
- 1470-8744
- OpenAlex
- W2062014263
- Cited by
- 24
- References
- 92
- Citations by year
- 2023: 1 · 2021: 2 · 2018: 2 · 2017: 1 · 2015: 2 · 2013: 1
- FWCI
- 0.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Applied Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Portsmouth
- Imperial College London
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- nuclear proteins, gene delivery vectors, nuclear localization signal, histones, high mobility group proteins, DNA condensation, transfection efficiency, protein-DNA complex, nuclear translocation, lysosomotropic agents
- MeSH terms
- Animals, DNA, Genetic Vectors, Humans, Nuclear Proteins, Gene Transfer Techniques
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