Cited by 24RNA Interference and Gene DeliveryVirus-based gene therapy researchNuclear Structure and Function

Nuclear proteins as gene-transfer vectors

Haberland, Annekathrin, Böttger, Michael·2005·Biotechnology and Applied Biochemistry

Abstract

An interest in nuclear proteins as possible gene vectors arose as early as 1988. Nuclear proteins possess important features, such as DNA condensing capacity and the presence of NLSs (nuclear localization signals), which are thought to be perfect tools to combine cellular and nuclear uptake of DNA. The NLS of the proteins might support the intracellular trafficking of the complexes, whereas their condensing capacity enables DNase stability. Nuclear proteins, histones and also non-histones, such as the high-mobility-group proteins, have been successfully employed in in vitro gene transfer by a variety of workers over the last decade. In the pertinent published data, almost all nuclear proteins tested showed gene-transfer activity. The degree of activity of the individual nuclear proteins tested varied according to sequential and structural differences of the proteins, discriminating their DNA binding behaviour, but reported transfection efficiency differences are inconsistent. They do not reveal a logical pattern, such as a correlation with their DNA affinity. The differences in transfection efficiency of certain nuclear proteins reported by different authors could be based on a lot of factors, including the source or method of protein preparation, the cells used, the media or the transfection additives, such as lysosomotropic agents. The variances are quite random, which suggests an involvement of physicochemical parameters of the nuclear protein-DNA complexes in the efficiency of gene expression. Physicochemical features of the complexes are also determined by factors such as transfection additives (e.g. Ca(2+)). The present review therefore includes, in addition to an overview about the nuclear proteins used in gene-transfer experiments, some assumptions about the mechanism of the cellular uptake of nuclear protein-DNA complexes and their translocation to the nucleus.

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Crossref · 10.1042/ba20050063

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Authors
Haberland, A., & Böttger, M.
Affiliations
  • Freie Universität Berlin (DE)
  • Max Delbrück Center (DE)
Venue
Biotechnology and Applied Biochemistry
Publisher
Wiley
Published
2005-10
Volume
42
Issue
2
Pages
97-106
ISSN
  • 0885-4513 (ISSN-L)
  • 1470-8744
OpenAlex
W2062014263
Cited by
24
References
92
Citations by year
2023: 1 · 2021: 2 · 2018: 2 · 2017: 1 · 2015: 2 · 2013: 1
FWCI
0.27
Type
journal article
Language
en
Open access
closed
Locations
  • Biotechnology and Applied Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Portsmouth
  • Imperial College London
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
nuclear proteins, gene delivery vectors, nuclear localization signal, histones, high mobility group proteins, DNA condensation, transfection efficiency, protein-DNA complex, nuclear translocation, lysosomotropic agents
MeSH terms
Animals, DNA, Genetic Vectors, Humans, Nuclear Proteins, Gene Transfer Techniques

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