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The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. The effects of changes in conformation
Open accessAbstract
1. The effects of changes in experimental conditions on the mobility of RNA in polyacrylamide-gel electrophoresis were investigated. 2. The linear relation between log(molecular weight) and electrophoretic mobility was shown to be independent within limits of salt or gel concentration. 3. The relative mobility of RNA with low content of guanylic acid and cytidylic acid residues was decreased in low-ionic-strength buffer. This was related to a small relative decrease in sedimentation coefficient. 4. However, Mg2+ ion caused almost no increase in mobility although it was associated with large increases in sedimentation coefficient. This suggested opposing actions of Mg2+ ion on the size and effective charge of the RNA. 5. It is concluded that the method provides a satisfactory measurement of molecular weight, which is almost independent of the nucleotide composition of RNA at moderate salt concentrations.
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Citations
Cited by
Detection of specific sequences among DNA fragments separated by gel electrophoresis
Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,249
Edmonds, M., Vaughan, M. H., & Nakazato, H. (1971) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.68.6.1336 · Cited by 510
Southern, E. (2006) · Nature Protocols · doi:10.1038/nprot.2006.73 · Cited by 230
Chowdhury, S. I., Kubin, G., & Ludwig, H. (1986) · Archives of Virology · doi:10.1007/bf01317376 · Cited by 68
Metadata
- Authors
- Loening, U. E.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1969-06-01
- Volume
- 113
- Issue
- 1
- Pages
- 131-138
- ISSN
- 0306-3283 (ISSN-L)
- OpenAlex
- W1833411966
- Cited by
- 768
- References
- 0
- FWCI
- 30.29
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Loening, U. E. (1969). The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. The effects of changes in conformation. Biochemical Journal. https://doi.org/10.1042/bj1130131
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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