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Isolation of choline esters from aqueous solutions by extraction with sodium tetraphenylboron in organic solvents
Open accessAbstract
1. The method is based on the observation that choline esters and sodium tetraphenylboron (Kalignost) form complexes that are insoluble in water but soluble in organic solvents such as nitriles, higher ketones and benzyl alcohol. 2. The extraction procedure is an example of liquid cation exchange where tetraphenylboron is the cation-exchange group. 3. The proportion of choline esters extracted depends on the type and total amount of cation in the aqueous phase and the amount of sodium tetraphenylboron in the organic solvent. 4. The proportion of choline esters extracted is independent of the choline ester concentration, the pH (between 8 and 3) and the relative volumes of the two phases. 5. The affinity of sodium tetraphenylboron for choline esters increases with an increase in the size of the acyl group. 6. The choline ester extracted can be released into an aqueous solution by treatment with strong acids, silver salts and anion-exchange resins.
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Cited by
A rapid radiochemical method for the determination of choline acetyltransferase
Fonnum, F. (1975) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1975.tb11895.x · Cited by 2,390
Self-citationThe functions of acetylcholine in the rabbit retina
Masland, R. H., Mills, J. W., & Cassidy, C. (1984) · Proceedings of the Royal Society of London. Series B. Biological Sciences · doi:10.1098/rspb.1984.0086 · Cited by 200
Grady, S. R., Meinerz, N. M., Cao, J., Reynolds, A. M., Picciotto, M. R., Changeux, J., McIntosh, J. M., Marks, M. J., & Collins, A. C. (2001) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2001.00019.x · Cited by 156
Kobayashi, H., Suzuki, T., Kasashima, Y., Motegi, A., Sato, I., Matsusaka, N., Ono, N., Miura, A., Saito, F., & Saito, S.-Y. (1996) · Japanese Journal of Pharmacology · doi:10.1254/jjp.72.317 · Cited by 24
Metadata
- Authors
- Fonnum, F.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1969-06-01
- Volume
- 113
- Issue
- 2
- Pages
- 291-298
- ISSN
- 0306-3283 (ISSN-L)
- OpenAlex
- W2107277845
- Cited by
- 217
- References
- 0
- FWCI
- 9.24
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Fonnum, F. (1969). Isolation of choline esters from aqueous solutions by extraction with sodium tetraphenylboron in organic solvents. Biochemical Journal. https://doi.org/10.1042/bj1130291
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