Enzyme synthesis in myxamoebae of the cellular slime mould Dictyostelium discoideum during growth in axenic culture
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1. The specific activities of β-N-acetylglucosaminidase, acid phosphatase, α-mannosidase, β-glucosidase, UDP-glucose pyrophosphorylase and alkaline phosphatase have been determined in myxamoebae of the cellular slime mould Dictyostelium discoideum Ax-2 grown on different media and in different phases of the growth cycle. 2. Variations in enzymic composition occur with changes in growth medium and phase of the growth cycle. 3. The intracellular location of the enzymes studied has been determined. 4. Two enzymes, β-N-acetylglucosaminidase and α-mannosidase, are not only synthesized preferentially as the myxamoebae enter the stationary phase of growth but they are also excreted. The excretion process appears to be specific, because other enzymes that occur in the same intracellular fraction are not excreted.
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Cells growing on bacteria do not express glycosidases, which are instead expressed in cells growing axenically.
“in agreement with previous results showing that cells growing on bacteria do not express glycosidases, which are instead expressed in cells growing axenically [48,49].”
From Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium (2008) · cites this paper as [48]
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- Authors
- Ashworth, J. M., & Quance, J.
- Affiliations
- University of Leicester (GB)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1972-02-01
- Volume
- 126
- Issue
- 3
- Pages
- 601-608
- ISSN
- 0306-3283 (ISSN-L)
- PMC
- PMC1178417
- OpenAlex
- W2238958467
- Cited by
- 80
- References
- 13
- Citations by year
- 2025: 1 · 2022: 1 · 2020: 1 · 2017: 1
- FWCI
- 11.79
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Slime Mold and Myxomycetes Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- Dictyostelium discoideum, myxamoebae, enzyme synthesis, N-acetyl-beta-D-glucosaminidase, acid phosphatase, alpha-mannosidase, β-glucosidase, UDP-glucose pyrophosphorylase, alkaline phosphatase, enzyme excretion, stationary phase, growth medium effects, enzyme subcellular localization, growth cycle
- MeSH terms
- Acid Phosphatase, Alkaline Phosphatase, Cell Fractionation, Glucosidases, Hexosaminidases, Lysosomes, Microsomes, Myxomycetes, Nucleotidyltransferases, Sodium Dodecyl Sulfate, Surface-Active Agents
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