Polypeptide-chain-elongation rate in Escherichia coli B/r as a function of growth rate
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By evaluating the kinetics of radioactive labelling of nascent and finished polypeptides, the peptide-chain elongation rate for Escherichia coli B/r at three different growth rates (mu) was determined to be 17 amino acids/s for the fast-growing cells (mu equals 1.3 and 2.0 doublings/h) and 12 amino acids/s for slow-growing cells (mu equals 0.67 doublings/h). The results agree with the growth-rate-dependence of the rate of peptide-chain elongation found for the translation of newly induced beta-galactosidase messenger in this strain and under these conditions of growth [Dalbow & Young (1975) Biochem. J. 150, 13-20]. Together with the previously observed ribosome efficiency at these growth rates [Dennis & Bremer (1974) J. Mol. Biol. 84, 407-422] the results indicate that the fraction of ribosomes engaged in protein synthesis is about 0.8 at all three growth rates.
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The translation speed was set to 12 aa/s.
“The translation speed was set to 12 aa/s.”
From Bringing the genetically minimal cell to life on a computer in 4D (2026) · cites this paper as [92]
The Results report a translation rate of about 12 amino acids/s for slow-growing E. coli B/r grown on succinate. The claim omits that condition: the paper reports about 17 amino acids/s for faster-growing cells. Our copy does not give a page number for the Results passage. (LLM, conf=0.88)
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- Authors
- Young, R., & Bremer, H.
- Affiliations
- The University of Texas at Dallas (US)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1976-11-15
- Volume
- 160
- Issue
- 2
- Pages
- 185-194
- ISSN
- 0306-3283 (ISSN-L)
- PMC
- PMC1164221
- OpenAlex
- W88241651
- Cited by
- 182
- References
- 25
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 7 · 2023: 9 · 2022: 13 · 2021: 18 · 2020: 11 · 2019: 8 · 2018: 11 · 2017: 13 · 2016: 13 · 2015: 5 · 2014: 13 · 2013: 9 · 2012: 11
- FWCI
- 1.12
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- Escherichia coli B/r, growth rate, translation kinetics, protein synthesis, beta-galactosidase induction, ribosome occupancy
- MeSH terms
- Bacterial Proteins, Enzyme Induction, Escherichia coli, Galactosidases, Kinetics, Models, Biological, Molecular Weight, Peptide Chain Elongation, Translational, Ribosomes
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