Cited by 182RNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyBiochemical and Molecular Research

Polypeptide-chain-elongation rate in Escherichia coli B/r as a function of growth rate

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Young, R, Bremer, H·1976·Biochemical Journal

Abstract

By evaluating the kinetics of radioactive labelling of nascent and finished polypeptides, the peptide-chain elongation rate for Escherichia coli B/r at three different growth rates (mu) was determined to be 17 amino acids/s for the fast-growing cells (mu equals 1.3 and 2.0 doublings/h) and 12 amino acids/s for slow-growing cells (mu equals 0.67 doublings/h). The results agree with the growth-rate-dependence of the rate of peptide-chain elongation found for the translation of newly induced beta-galactosidase messenger in this strain and under these conditions of growth [Dalbow & Young (1975) Biochem. J. 150, 13-20]. Together with the previously observed ribosome efficiency at these growth rates [Dennis & Bremer (1974) J. Mol. Biol. 84, 407-422] the results indicate that the fraction of ribosomes engaged in protein synthesis is about 0.8 at all three growth rates.

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  • The translation speed was set to 12 aa/s.

    “The translation speed was set to 12 aa/s.”

    From Bringing the genetically minimal cell to life on a computer in 4D (2026) · cites this paper as [92]

    The Results report a translation rate of about 12 amino acids/s for slow-growing E. coli B/r grown on succinate. The claim omits that condition: the paper reports about 17 amino acids/s for faster-growing cells. Our copy does not give a page number for the Results passage. (LLM, conf=0.88)

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Authors
Young, R., & Bremer, H.
Affiliations
The University of Texas at Dallas (US)
Venue
Biochemical Journal
Published
1976-11-15
Volume
160
Issue
2
Pages
185-194
ISSN
0306-3283 (ISSN-L)
OpenAlex
W88241651
Cited by
182
References
25
Citations by year
2026: 6 · 2025: 6 · 2024: 7 · 2023: 9 · 2022: 13 · 2021: 18 · 2020: 11 · 2019: 8 · 2018: 11 · 2017: 13 · 2016: 13 · 2015: 5 · 2014: 13 · 2013: 9 · 2012: 11
FWCI
1.12
Type
journal article
Language
en
Open access
Locations
  • Biochemical Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
Keywords
Escherichia coli B/r, growth rate, translation kinetics, protein synthesis, beta-galactosidase induction, ribosome occupancy
MeSH terms
Bacterial Proteins, Enzyme Induction, Escherichia coli, Galactosidases, Kinetics, Models, Biological, Molecular Weight, Peptide Chain Elongation, Translational, Ribosomes

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