The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Rapid isolation of plasma membranes in high yield from cultured fibroblasts
Open accessAbstract
1. A method was developed which allows the rapid preparation of pure plasma membranes in high yield from cultured fibroblasts. 2. Cells are lysed in hypo-osmotic borate/EDTA and, after differential centrifugation, the membranes collected by centrifugation on a sucrose barrier. 3. Electron microscopy of the isolated material shows large membrane vesicles essentially free from contaminating organelles. 4. There is no detectable activity of the endoplasmic-reticulum enzyme marker, NADH2—lipoamide oxidoreductase (EC 1.6.4.3), and that of succinate dehydrogenase (EC 1.3.99.1), a marker for mitochondria, is substantially decreased. Chemical compositions are in good agreement with previous observations. 5. This study confirms the usefulness of applied isotopic markers for isolating plasma membranes.
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Cites
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Cited by
Stimulation of tyrosine-specific phosphorylation by platelet-derived growth factor
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Epidermal growth factor stimulates phosphorylation in membrane preparations in vitro
CARPENTER, G., KING, L., & COHEN, S. (1978) · Nature · doi:10.1038/276409a0 · Cited by 454
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Metadata
- Authors
- Thom, D., Powell, A. J., Lloyd, C. W., & Rees, D. A.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1977-11-15
- Volume
- 168
- Issue
- 2
- Pages
- 187-194
- ISSN
- 0306-3283 (ISSN-L)
- OpenAlex
- W126219881
- Cited by
- 302
- References
- 12
- FWCI
- 2.47
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Thom, D., Powell, A. J., Lloyd, C. W., & Rees, D. A. (1977). Rapid isolation of plasma membranes in high yield from cultured fibroblasts. Biochemical Journal. https://doi.org/10.1042/bj1680187
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
