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Cited by 488

The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.

Studies on the Chemical and Enzymatic Modification of Glycoproteins

Open access
Van Lenten, Lee, Ashwell, Gilbert·1971·Journal of Biological Chemistry

Abstract

Abstract A general method is described for the radioactive labeling of proteins containing terminal sialic acid in the carbohydrate moieties. Quantitative conversion of these residues to a radioactive 7-carbon analogue of sialic acid was achieved by sequential periodate oxidation and tritiated borohydride reduction. Evidence is presented to establish the identity of the radioactive derivative as 5-acetamido-3, 5-dideoxy-l-arabino-2-heptulosonic acid and to indicate it as the sole site of tritium incorporation in the carbohydrate chain. Upon injection into rats, labeled preparations of ceruloplasmin and orosomucoid exhibited a normal half-life in the plasma and proved suitable for metabolic studies. The data indicate that the 7-carbon sialic acid analogue mimics sialic acid with respect to hydrolysis by neuraminidase and in regulating survival of glycoproteins in the circulation.

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Crossref · 10.1016/s0021-9258(18)62392-0

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Metadata

Authors
Van Lenten, L., & Ashwell, G.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1971-03
Volume
246
Issue
6
Pages
1889-1894
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1566087596
Cited by
488
References
16
FWCI
7.46
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Studies on the Chemical and Enzymatic Modification of Glycoproteins. https://app.cochat.ai/papers/10.1016/s0021-9258(18)62392-0
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Van Lenten, L., & Ashwell, G. (1971). Studies on the Chemical and Enzymatic Modification of Glycoproteins. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)62392-0

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.