Cited by 29Amino Acid Enzymes and MetabolismBiochemical and Molecular ResearchCancer, Hypoxia, and Metabolism

Effect of glutamine on the degradation of glutamine synthetase in hepatoma tissue-culture cells

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Crook, R B, Tomkins, G M·1978·Biochemical Journal

Abstract

In certain lines of hepatoma tissue-culture cells, the extracellular glutamine concentration regulates the specific activity of glutamine synthetase. By quantifying the radioactivity in immunoprecipitated glutamine synthetase on polyacrylamide gels, we found that the rate of degradation, but not of synthesis, of glutamine synthetase is a sensitive function of extracellular glutamine. The activiy that degrades this enzyme appears to be labile.

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Crossref · 10.1042/bj1760047

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  • GS is subject to feedback control by glutamine, which promotes its post-translational modification and degradation.

    “It has been shown that GS is subject to feedback control by glutamine, which promotes its post-translational modification and degradation (Arad et al., 1976; Crook and Tomkins, 1978).”

    From Glutamine Triggers Acetylation-Dependent Degradation of Glutamine Synthetase via the Thalidomide Receptor Cereblon (2016) · cites this paper as Crook and Tomkins, 1978

    The Abstract and Discussion report that extracellular glutamine regulates glutamine synthetase activity by changing its rate of degradation in hepatoma tissue-culture cells. The Discussion says the degradation mechanism is unknown: covalent modification is only a possibility, and the authors consider proteolytic cleavage more compatible with their data. The claim's assertion that glutamine promotes post-translational modification is therefore not established by this paper. (LLM, conf=0.90)

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1 of the 14 references couldn’t be matched to a known paper.

Research works printed but not linked: 2

  1. Crook, R. B., Louie, M., Deuel, T. & Tomkins, G. M. (1978) J. Biol. Chem. in the press
  2. Peterkofsky, B. & Tomkins, G. (1968) Proc. Nati. Acad. Sci. U.S.A. 60, 222-228

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Authors
Crook, R. B., & Tomkins, G. M.
Affiliations
University of California, San Francisco (US)
Venue
Biochemical Journal
Published
1978-10-15
Volume
176
Issue
1
Pages
47-52
ISSN
0264-6021 (ISSN-L)
OpenAlex
W117334402
Cited by
29
References
14
Citations by year
2026: 1 · 2023: 1 · 2021: 2 · 2019: 1 · 2017: 1 · 2016: 3
FWCI
0.38
Type
journal article
Language
en
Open access
Locations
  • Biochemical Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
Keywords
glutamine synthetase, enzyme degradation, glutamine, hepatoma cells, enzyme regulation, protein turnover, enzyme synthesis
MeSH terms
Animals, Cell Line, Cycloheximide, Dactinomycin, Glutamine, Glutamate-Ammonia Ligase, Kinetics, Liver Neoplasms, Experimental

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