Effect of glutamine on the degradation of glutamine synthetase in hepatoma tissue-culture cells
Open accessAbstract
In certain lines of hepatoma tissue-culture cells, the extracellular glutamine concentration regulates the specific activity of glutamine synthetase. By quantifying the radioactivity in immunoprecipitated glutamine synthetase on polyacrylamide gels, we found that the rate of degradation, but not of synthesis, of glutamine synthetase is a sensitive function of extracellular glutamine. The activiy that degrades this enzyme appears to be labile.
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GS is subject to feedback control by glutamine, which promotes its post-translational modification and degradation.
“It has been shown that GS is subject to feedback control by glutamine, which promotes its post-translational modification and degradation (Arad et al., 1976; Crook and Tomkins, 1978).”
From Glutamine Triggers Acetylation-Dependent Degradation of Glutamine Synthetase via the Thalidomide Receptor Cereblon (2016) · cites this paper as Crook and Tomkins, 1978
The Abstract and Discussion report that extracellular glutamine regulates glutamine synthetase activity by changing its rate of degradation in hepatoma tissue-culture cells. The Discussion says the degradation mechanism is unknown: covalent modification is only a possibility, and the authors consider proteolytic cleavage more compatible with their data. The claim's assertion that glutamine promotes post-translational modification is therefore not established by this paper. (LLM, conf=0.90)
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- Authors
- Crook, R. B., & Tomkins, G. M.
- Affiliations
- University of California, San Francisco (US)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1978-10-15
- Volume
- 176
- Issue
- 1
- Pages
- 47-52
- ISSN
- 0264-6021 (ISSN-L)
- PMC
- PMC1186203
- OpenAlex
- W117334402
- Cited by
- 29
- References
- 14
- Citations by year
- 2026: 1 · 2023: 1 · 2021: 2 · 2019: 1 · 2017: 1 · 2016: 3
- FWCI
- 0.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Keywords
- glutamine synthetase, enzyme degradation, glutamine, hepatoma cells, enzyme regulation, protein turnover, enzyme synthesis
- MeSH terms
- Animals, Cell Line, Cycloheximide, Dactinomycin, Glutamine, Glutamate-Ammonia Ligase, Kinetics, Liver Neoplasms, Experimental
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