The DOI matches its Crossref record; 102 of the 103 references it cites were found in a registry or an index.
Focusing on clathrin-mediated endocytosis.
Abstract
Investigations into the mechanisms which regulate entry of integral membrane proteins, and associated ligands, into the cell through vesicular carriers (endocytosis) have greatly benefited from the application of live-cell imaging. Several excellent recent reviews have detailed specific aspects of endocytosis, such as entry of particular cargo, or the different routes of internalization. The aim of the present review is to highlight how advances in live-cell fluorescence microscopy have affected the study of clathrin-mediated endocytosis. The last decade has seen a tremendous increase in the development and dissemination of methods for imaging endocytosis in live cells, and this has been followed by a dramatic shift in the way this critical cellular pathway is studied and understood. The present review begins with a description of the technical advances which have permitted new types of experiment to be performed, as well as potential pitfalls of these new technologies. Subsequently, advances in the understanding of three key endocytic proteins will be addressed: clathrin, dynamin and AP-2 (adaptor protein 2). Although great strides have clearly been made in these areas in recent years, as is often the case, each answer has bred numerous questions. Furthermore, several examples are highlighted where, because of seemingly minor differences in experimental systems, what appear at first to be very similar studies have, at times, yielded vastly differing results and conclusions. Thus this is an exceedingly exciting time to study endocytosis, and this area serves as a clear demonstration of the power of applying live-cell imaging to answer fundamental biological questions.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Receptor-mediated internalization of cargo followed by vesicular transport is a suitable option as nano-delivery systems of size 100–200 nm range can be easily accommodated in these transport vesicles”
From Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs (2020) · cites this paper as Rappoport, 2008
In the citing paper
“Clathrin-mediated endocytosis happens in all mammalian cells.”
From Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs (2020) · cites this paper as Rappoport, 2008
Citations
Cites
Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy
Huang, B., Wang, W.-Q., Bates, M., & Zhuang, X. (2008) · Science · doi:10.1126/science.1153529 · Cited by 2,830
Multicolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes
Bates, M., Huang, B., Dempsey, G. T., & Zhuang, X. (2007) · Science · doi:10.1126/science.1146598 · Cited by 1,507
YOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L
Roth, T. F., & Porter, K. R. (1964) · The Journal of Cell Biology · doi:10.1083/jcb.20.2.313 · Cited by 1,320
Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER
Pelkmans, L., Kartenbeck, J., & Helenius, A. (2001) · Nature Cell Biology · doi:10.1038/35074539 · Cited by 1,236
Induction of mutant dynamin specifically blocks endocytic coated vesicle formation.
Damke, H., Baba, T., Warnock, D. E., & Schmid, S. L. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.127.4.915 · Cited by 1,203
Studying protein dynamics in living cells
Lippincott‐Schwartz, J., Snapp, E. L., & Kenworthy, A. K. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35073068 · Cited by 1,192
Curvature of clathrin-coated pits driven by epsin
Ford, M. G. J., Mills, I. G., Peter, B. J., Vallis, Y., Praefcke, G. J. K., Evans, P. R., & McMahon, H. T. (2002) · Nature · doi:10.1038/nature01020 · Cited by 1,019
Development and Use of Fluorescent Protein Markers in Living Cells
Lippincott‐Schwartz, J., & Patterson, G. H. (2003) · Science · doi:10.1126/science.1082520 · Cited by 993
Total Internal Reflection Fluorescence Microscopy in Cell Biology
Axelrod, D. (2001) · Traffic · doi:10.1034/j.1600-0854.2001.21104.x · Cited by 968
Hypertonic media inhibit receptor-mediated endocytosis by blocking clathrin-coated pit formation.
Heuser, J. E., & Anderson, R. G. W. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.108.2.389 · Cited by 924
Cited by
Sorted by citations
Sahay, G., Alakhova, D. Y., & Kabanov, A. V. (2010) · Journal of Controlled Release · doi:10.1016/j.jconrel.2010.01.036 · Cited by 2,181
Insight into Cellular Uptake and Intracellular Trafficking of Nanoparticles
Foroozandeh, P., & Aziz, A. A. (2018) · Nanoscale Research Letters · doi:10.1186/s11671-018-2728-6 · Cited by 1,438
Cellular Uptake, Intracellular Trafficking, and Cytotoxicity of Nanomaterials
Zhao, F., Zhao, Y., Liu, Y., Chang, X., Chen, C., & Zhao, Y. (2011) · Small · doi:10.1002/smll.201100001 · Cited by 1,206
A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis
Taylor, M. J., Perrais, D., & Merrifield, C. J. (2011) · PLoS Biology · doi:10.1371/journal.pbio.1000604 · Cited by 754
Lipids and Lipid Derivatives for RNA Delivery
Zhang, Y., Sun, C., Wang, C., Jankovic, K. E., & Dong, Y. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00244 · Cited by 649
Metadata
- Authors
- Rappoport, J. Z.
- Affiliations
- University of Birmingham (GB)
- Corresponding author
- Rappoport, Joshua Z.
- ORCID
- Rappoport, Joshua Z.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 2008-05-28
- Volume
- 412
- Issue
- 3
- Pages
- 415-423
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W2012515487
- Cited by
- 172
- References
- 103
- Citations by year
- 2025: 4 · 2024: 8 · 2023: 5 · 2022: 6 · 2021: 6 · 2020: 14 · 2019: 6 · 2018: 5 · 2017: 16 · 2016: 11 · 2015: 10 · 2014: 12 · 2013: 9 · 2012: 14
- FWCI
- 1.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical Journal (publishedVersion)
- PubMed (publishedVersion)
- University of Birmingham Research Portal (University of Birmingham) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
- Keywords
- clathrin-mediated endocytosis, live-cell fluorescence microscopy, clathrin, dynamin, vesicle trafficking, endocytic proteins
- MeSH terms
- Animals, Clathrin, Endocytosis, Humans, Microscopy, Fluorescence, Protein Transport, Clathrin-Coated Vesicles, Adaptor Protein Complex 2, Dynamins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
