The DOI matches its Crossref record; 50 of the 53 references it cites were found in a registry or an index.
Identification and characterization of FUS/TLS as a new target of ATM
Open accessAbstract
ATM (ataxia-telangiectasia mutated), ATR (ATM- and Rad3-related) and DNA-PK (DNA-dependent protein kinase), important regulators of genome stability, belong to the PIKK (phosphoinositide 3-kinase-like kinase) family of protein kinases. In the present study, DNA-affinity chromatography was used to identify DNA-binding proteins phosphorylated by these kinases. This resulted in the identification of FUS (fused in sarcoma)/TLS (translocated in liposarcoma) as an in vitro target of the PIKKs. FUS is a member of the Ewing's sarcoma family of proteins that appears to play a role in regulating genome stability, since mice lacking FUS show chromosomal instability and defects in meiosis. The residues in FUS that are phosphorylated in vitro and in vivo were identified, and phospho-specific antibodies were generated to demonstrate that FUS becomes phosphorylated at Ser42in vivo, primarily in response to agents that cause DSBs (double-strand breaks). DSB-induced FUS phosphorylation in vivo at Ser42 requires ATM and not DNA-PK. Although Ser42 is retained in the oncogenic FUS–CHOP [C/EBP (CCAAT/enhancer-binding protein)-homologous protein 10] fusion generated by a t(12;16)(q13;p11) chromosomal translocation, Ser42 in FUS–CHOP is not phosphorylated after DNA damage. These results identify FUS as a new target of the ATM-signalling pathway and strengthen the notion that FUS regulates genome stability.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Furthermore, phosphorylation of FUS occurs in response to DSB detection by proteins ATM and DNA‐PK (Gardiner et al, 2008; Deng et al, 2014)”
From Mechanisms of DNA damage-mediated neurotoxicity in neurodegenerative disease (2022) · cites this paper as Gardiner et al, 2008
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage
Matsuoka, S., Ballif, B. A., Smogorzewska, A., McDonald, E. R., Hurov, K. E., Luo, J., Bakalarski, C. E., Zhao, Z., Solimini, N., Lerenthal, Y., et al. (2007) · Science · doi:10.1126/science.1140321 · Cited by 3,075
ATM and related protein kinases: safeguarding genome integrity
Shiloh, Y. (2003) · Nature Reviews Cancer · doi:10.1038/nrc1011 · Cited by 2,577
Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage
Falck, J., Coates, J., & Jackson, S. P. (2005) · Nature · doi:10.1038/nature03442 · Cited by 1,301
Hickson, I., Zhao, Y., Richardson, C. J., Green, S. J., Martin, N. M. B., Orr, A. I., Reaper, P. M., Jackson, S. P., Curtin, N. J., & Smith, G. C. M. (2004) · Cancer Research · doi:10.1158/0008-5472.can-04-2727 · Cited by 1,220
Maris, C., Dominguez, C., & Allain, F. H. ‐. (2005) · FEBS Journal · doi:10.1111/j.1742-4658.2005.04653.x · Cited by 1,135
Sancar, A. (1996) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.65.070196.000355 · Cited by 1,131
The Bloom's syndrome helicase suppresses crossing over during homologous recombination
Wu, L., & Hickson, I. D. (2003) · Nature · doi:10.1038/nature02253 · Cited by 1,087
The DNA-dependent protein kinasePreprint
Smith, G. C. M., & Jackson, S. P. (1999) · Genes & Development · doi:10.1101/gad.13.8.916 · Cited by 903
Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma
Crozat, A., Åman, P., Mandahl, N., & Ron, D. (1993) · Nature · doi:10.1038/363640a0 · Cited by 897
Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 Protein
Sung, P. (1994) · Science · doi:10.1126/science.8066464 · Cited by 869
Flagged first, then by citations
3 of the 53 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Items 101–113 of 113
EWSR1 and FUS fusion genes in tumorigenesis
Möller, E. (2010) · Lund University Publications (Lund University)
Molecular analysis of the FET family fusion oncoprotein FUS-DDIT3
Thomsen, C. (2012) · Gothenburg University Publications Electronic Archive (Gothenburg University)
FET proteins in cancer and development
Andersson, M. K. (2009) · Gothenburg University Publications Electronic Archive (Gothenburg University)
Chen, M., Foster, J. P., Lock, I. C., Leisenring, N. H., Daniel, A. R., Floyd, W., Xu, H. E., Davis, I. J., & Kirsch, D. G. (2023) · doi:10.1158/0008-5472.c.6513181.v1
Physiological function of FUS: An RNA binding protein in motor neuron disease
Yang, L. (2015) · UKnowledge (University of Kentucky)
Zhong, C., Wang, H., Yuan, X., He, Y., Cong, J., Yang, R., Ma, W., Gao, L., Gao, C., Cui, Y., et al. (2024) · Figshare · doi:10.6084/m9.figshare.25951905
Zhong, C., Wang, H., Yuan, X., He, Y., Cong, J., Yang, R., Ma, W., Gao, L., Gao, C., Cui, Y., et al. (2024) · Figshare · doi:10.6084/m9.figshare.25951807
Conditional truncation of the FUS protein in mice : a new animal model of the ALS/FTD continuum
Scekic‐Zahirovic, J. (2016) · theses.fr (ABES) · doi:10.70675/6bed4f95z0a05z438fz937ez33f29f11923d
Picchiarelli, G. (2018) · theses.fr (ABES) · doi:10.70675/0252f66fz38f9z46b6z8f3ez97b2f3467520 · Cited by 1
Novel FUS and CHCHD10 models to investigate pathogenic mechanisms in Amyotrophic Lateral Sclerosis
Bourefis, A.-R. (2019) · theses.fr (ABES) · doi:10.70675/45ab8515z9859z430fzb472z306dd61206c8
The link between PARP1-mediated signaling and RNA-binding protein FUS in response to DNA damage
Singatulina, A. (2019) · theses.fr (ABES) · doi:10.70675/bdd8c1b6z3cfcz4fd8z9277z39ed8983ee72
Metadata
- Authors
- Gardiner, M., Toth, R., Vandermoere, F., Morrice, N. A., & Rouse, J.
- Affiliations
- University of Dundee (GB)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 2008-09-25
- Volume
- 415
- Issue
- 2
- Pages
- 297-307
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W2023532020
- Cited by
- 111
- References
- 53
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 3 · 2023: 8 · 2022: 6 · 2021: 12 · 2020: 7 · 2019: 8 · 2018: 9 · 2017: 6 · 2016: 11 · 2015: 4 · 2014: 6 · 2013: 4 · 2012: 5
- FWCI
- 2.47
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed (publishedVersion)
- Discovery Research Portal (University of Dundee) (publishedVersion)
- HAL (submittedVersion, other-oa)
- Trinity's Access to Research Output (TARA) (Trinity College Dublin) (submittedVersion, other-oa)
- Funders
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- FUS, TLS, ATM, PIKK kinases, protein phosphorylation, DNA double-strand breaks, DNA damage response, genome stability, chromosomal instability, DNA-binding proteins
- MeSH terms
- Amino Acid Sequence, Cell Line, Chromatography, Affinity, DNA Damage, DNA-Binding Proteins, Humans, Molecular Sequence Data, Phosphorylation, Recombinant Fusion Proteins, Serine, Mass Spectrometry, Ultraviolet Rays, Protein Serine-Threonine Kinases, Sequence Homology, Amino Acid, Cell Cycle Proteins, Tumor Suppressor Proteins, RNA-Binding Protein FUS, DNA-Activated Protein Kinase, Ataxia Telangiectasia Mutated Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
