The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Far-red fluorescent tags for protein imaging in living tissues
Open accessAbstract
A vast colour palette of monomeric fluorescent proteins has been developed to investigate protein localization, motility and interactions. However, low brightness has remained a problem in far-red variants, which hampers multicolour labelling and whole-body imaging techniques. In the present paper, we report mKate2, a monomeric far-red fluorescent protein that is almost 3-fold brighter than the previously reported mKate and is 10-fold brighter than mPlum. The high-brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior fluorescent tag for imaging in living tissues. We also report tdKatushka2, a tandem far-red tag that performs well in fusions, provides 4-fold brighter near-IR fluorescence compared with mRaspberry or mCherry, and is 20-fold brighter than mPlum. Together, monomeric mKate2 and pseudo-monomeric tdKatushka2 represent the next generation of extra-bright far-red fluorescent probes offering novel possibilities for fluorescent imaging of proteins in living cells and animals.
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Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004) · Nature Biotechnology · doi:10.1038/nbt1037 · Cited by 4,619
Cited by
Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues
Chudakov, D. M., Matz, M. V., Lukyanov, S., & Lukyanov, K. A. (2010) · Physiological Reviews · doi:10.1152/physrev.00038.2009 · Cited by 1,375
Self-citationmScarlet: a bright monomeric red fluorescent protein for cellular imaging
Bindels, D. S., Haarbosch, L., van Weeren, L., Postma, M., Wiese, K. E., Mastop, M., Aumonier, S., Gotthard, G., Royant, A., Hink, M. A., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4074 · Cited by 1,156
Astrocytes Control Breathing Through pH-Dependent Release of ATP
Gourine, A. V., Kasymov, V., Marina, N., Tang, F., Figueiredo, M. F., Lane, S., Teschemacher, A. G., Spyer, K. M., Deisseroth, K., & Kasparov, S. (2010) · Science · doi:10.1126/science.1190721 · Cited by 872
Bright and stable near-infrared fluorescent protein for in vivo imaging
Filonov, G. S., Piatkevich, K. D., Ting, L.-M., Zhang, J., Kim, K., & Verkhusha, V. V. (2011) · Nature Biotechnology · doi:10.1038/nbt.1918 · Cited by 688
Self-citationBright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging
Shcherbakova, D. M., Baloban, M., Emelyanov, A. V., Brenowitz, M., Guo, P., & Verkhusha, V. V. (2016) · Nature Communications · doi:10.1038/ncomms12405 · Cited by 312
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Metadata
- Authors
- Shcherbo, D., Murphy, C. S., Ermakova, G. V., Solovieva, E. A., Chepurnykh, T. V., Shcheglov, A. S., Verkhusha, V. V., Pletnev, V. Z., Hazelwood, K. L., Roche, P. M., Lukyanov, S., Zaraisky, A. G., Davidson, M. W., & Chudakov, D. M.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 2009-02-25
- Volume
- 418
- Issue
- 3
- Pages
- 567-574
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W2122683100
- Cited by
- 563
- References
- 1
- FWCI
- 18.19
- Type
- journal article
- Language
- en
- Open access
- green
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- Shcherbo, D., Murphy, C. S., Ermakova, G. V., Solovieva, E. A., Chepurnykh, T. V., Shcheglov, A. S., Verkhusha, V. V., Pletnev, V. Z., Hazelwood, K. L., Roche, P. M., Lukyanov, S., Zaraisky, A. G., Davidson, M. W., & Chudakov, D. M. (2009). Far-red fluorescent tags for protein imaging in living tissues. Biochemical Journal. https://doi.org/10.1042/bj20081949
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